LOCUS       sequence-383261-        9465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Co-expresses MBP-GSK3-beta_S9A-HA-His (human GSK3-beta with S9A 
            mutation as a fusion protein with MBP, HA, and His-tags) and 
            GST_lambda-PPase in E.coli to produce unphosphorylated 
            MBP-GSK3-beta_S9A-HA-His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Gavagan M, Jameson N, Zalatan JG
  TITLE     The Axin scaffold protects the kinase GSK3beta from cross-pathway 
            inhibition.
  JOURNAL   Elife. 2023 Aug 7;12:e85444. doi: 10.7554/eLife.85444.
  PUBMED    37548359
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-08-7"; volume: "12:e85444. doi"; pages: " 
            10.7554/eLife.85444"
COMMENT     Sequence Label: pMG071 MBP-GSK3-beta_S9A-HA-His, GST_lambda-PPase
FEATURES             Location/Qualifiers
     source          1..9465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      2485..2494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2677..2688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2731..2751
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3569..3580
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4015..4041
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4021..4047
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4069..4086
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             4116..4138
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4161..4811
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPP"
     primer_bind     4772..4794
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             4848..4868
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(5558..5575)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5558..5574)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5567..5589)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5753..5770)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5923..6009
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6101..6128
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        6147..6238
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6239..6307,6308..7099)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6457..6476)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(7141..7654)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7336..7357)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7548..7567
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7765..8353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8254..8273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8507..8524
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8539..8679
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8605..8624)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8765..8787
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8781..8972)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagattatg aaaactgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat ttcaactagt
     2701 ggatctggtg gtggtggcgg ttccatgagc gagaatcttt attttcaggg atcctcaggg
     2761 cggcccagaa ccaccgcctt tgcggagagc tgcaagccgg tgcagcagcc ttcagctttt
     2821 ggcagcatga aagttagcag agacaaggac ggcagcaagg tgacaacagt ggtggcaact
     2881 cctgggcagg gtccagacag gccacaagaa gtcagctata cagacactaa agtgattgga
     2941 aatggatcat ttggtgtggt atatcaagcc aaactttgtg attcaggaga actggtcgcc
     3001 atcaagaaag tattgcagga caagagattt aagaatcgag agctccagat catgagaaag
     3061 ctagatcact gtaacatagt ccgattgcgt tatttcttct actccagtgg tgagaagaaa
     3121 gatgaggtct atcttaatct ggtgctggac tatgttccgg aaacagtata cagagttgcc
     3181 agacactata gtcgagccaa acagacgctc cctgtgattt atgtcaagtt gtatatgtat
     3241 cagctgttcc gaagtttagc ctatatccat tcctttggaa tctgccatcg ggatattaaa
     3301 ccgcagaacc tcttgttgga tcctgatact gctgtattaa aactctgtga ctttggaagt
     3361 gcaaagcagc tggtccgagg agaacccaat gtttcgtata tctgttctcg gtactatagg
     3421 gcaccagagt tgatctttgg agccactgat tatacctcta gtatagatgt atggtctgct
     3481 ggctgtgtgt tggctgagct gttactagga caaccaatat ttccagggga tagtggtgtg
     3541 gatcagttgg tagaaataat caaggtcctg ggaactccaa caagggagca aatcagagaa
     3601 atgaacccaa actacacaga atttaaattc cctcaaatta aggcacatcc ttggactaag
     3661 gtcttccgac cccgaactcc accggaggca attgcactgt gtagccgtct gctggagtat
     3721 acaccaactg cccgactaac accactggaa gcttgtgcac attcattttt tgatgaatta
     3781 cgggacccaa atgtcaaact accaaatggg cgagacacac ctgcactctt caacttcacc
     3841 actcaagaac tgtcaagtaa tccacctctg gctaccatcc ttattcctcc tcatgctcgg
     3901 attcaagcag ctgcttcaac ccccacaaat gccacagcag cgtcagatgc taatactgga
     3961 gaccgtggac agaccaataa tgctgcttct gcatcagctt ccaactccac tagttaccca
     4021 tacgatgttc cagattacgc aagcttgggt ggtcccaaca gcggccgcca ccatcaccac
     4081 caccactagg ggccgcactc gaggctagaa ataattttgt ttaactttaa gaaggagata
     4141 tacatatgaa acaagatctg atgtccccta tactaggtta ttggaaaatt aagggccttg
     4201 tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg
     4261 agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca
     4321 atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt
     4381 atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa
     4441 tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag
     4501 actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg
     4561 aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca
     4621 tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc
     4681 caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga
     4741 aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg
     4801 accatcctcc aactagtgga tctggtggtg gtggcggttc catgagcgag aatctttatt
     4861 ttcagggatc catgcgctat tacgaaaaaa ttgatggcag caaataccga aatatttggg
     4921 tagttggcga tctgcacgga tgctacacga acctgatgaa caaactggat acgattggat
     4981 tcgacaacaa aaaagacctg cttatctcgg tgggcgattt ggttgatcgt ggtgcagaga
     5041 acgttgaatg cctggaatta atcacattcc cctggttcag agctgtacgt ggaaaccatg
     5101 agcaaatgat gattgatggc ttatcagagc gtggaaacgt taatcactgg ctgcttaatg
     5161 gcggtggctg gttctttaat ctcgattacg acaaagaaat tctggctaaa gctcttgccc
     5221 ataaagcaga tgaacttccg ttaatcatcg aactggtgag caaagataaa aaatatgtta
     5281 tctgccacgc cgattatccc tttgacgaat acgagtttgg aaagccagtt gatcatcagc
     5341 aggtaatctg gaaccgcgaa cgaatcagca actcacaaaa cgggatcgtg aaagaaatca
     5401 aaggcgcgga cacgttcatc tttggtcata cgccagcagt gaaaccactc aagtttgcca
     5461 accaaatgta tatcgatacc ggcgcagtgt tctgcggaaa cctaacattg attcaggtac
     5521 agggagaagg cgcatagctg cagtcgagaa gcttggcact ggccgtcgtt ttacaacgtc
     5581 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5641 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5701 tgaatggcga atggcagctt ggctgttttg gcggatgaga taagattttc agcctgatac
     5761 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     5821 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     5881 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     5941 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     6001 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     6061 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     6121 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
     6181 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6241 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6301 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6361 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6421 agaacgtttc ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6481 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6541 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6601 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6661 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6721 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6781 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6841 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6901 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6961 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7021 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7081 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7141 accccggttg ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa
     7201 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     7261 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gcccgagata
     7321 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     7381 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa
     7441 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     7501 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     7561 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     7621 cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga agatcctttt
     7681 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7741 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7801 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7861 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7921 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7981 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8041 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8101 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8161 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8221 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8281 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8341 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8401 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8461 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8521 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     8581 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8641 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
     8701 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     8761 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
     8821 ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac agatgtctgc ctgttcatcc
     8881 gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc
     8941 atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaatg gggatttctg
     9001 ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat
     9061 gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg
     9121 cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt
     9181 gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc
     9241 gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt
     9301 gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat
     9361 tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc
     9421 acgatcatgc gcacccgtgg ccaggaccca acgctgcccg aaatt
//
