LOCUS       sequence-38327-e         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Sanjana NE, Cong L, Zhou Y, Cunniff MM, Feng G, Zhang F
  TITLE     A transcription activator-like effector toolbox for genome 
            engineering.
  JOURNAL   Nat Protoc. 2012 Jan 5;7(1):171-92. doi: 10.1038/nprot.2011.431.
  PUBMED    22222791
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2011"; journalName: "Nat Protoc"; date: "2012-01-5- 
            5"; volume: "7"; issue: "1"; pages: "171-92"
COMMENT     Sequence Label: pNG_v2
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(120..139)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        206..297
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctggcagtt ccctactctc gcatggggag tccccacact accatcggcg ctacggcgtt
       61 tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaac
      121 aaggggtgtt atgagccata ttcaggtata aatgggctcg cgataatgtt cagaattggt
      181 taattggttg taacactgac ccctatttgt ttatttttct aaatacattc aaatatgtat
      241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagaatatg
      301 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
      361 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
      421 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
      481 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc acttccgacc
      541 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
      601 aaaacagcgt tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
      661 ctggcagtgt tcctgcgccg gttgcactcg attcctgttt gtaattgtcc ttttaacagc
      721 gatcgcgtat ttcgcctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
      781 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
      841 aaa
//
