LOCUS       sequence-383305-        2645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the protein of BDFP1.6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, 
            Koster RW, Wu L, Namikawa K, Piatkevich KD
  TITLE     Quantitative assessment of near-infrared fluorescent proteins.
  JOURNAL   Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z.
  PUBMED    37666982
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z."
COMMENT     Sequence Label: pAAV-CAG-BDFP1.6
FEATURES             Location/Qualifiers
     source          1..2645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        495..772
                     /label=chicken beta-actin promoter
     primer_bind     1051..1073
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1135..1154
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1193..1202
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1682..2270
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1735..1755)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2153..2164)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2313..2434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2324..2343)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2378..2397
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cagtaccatg acgtcaggcg gccatcgtgg tacgccgatc cgtgccggtc gctgattatt
       61 atgcagctgc ccgctcgctc gctcactgag ccgccgggca aagcccgggc gtcgggcgac
      121 tttgttcgcc cgccctcagt gaccgaccga accgccccag agaggagtgc actcatcact
      181 agggtctgta gtaatgatta acccgccatg ctactatcta cgtagccatg ctctaggaag
      241 agtaccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      301 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      361 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      421 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      481 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
      541 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
      601 gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
      661 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
      721 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
      781 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
      841 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      901 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
      961 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg cggggggacg
     1021 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1081 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1141 tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatccggta ccgccaccat
     1201 ggccaatcgc gaggttgttg agaccctgaa ggagctcctg gctgacgggg agaagagagt
     1261 gcaggtggct ggagtgattg ggactaacgc tgccgaagtt gtcaagaccg ccgtgtcact
     1321 tctgtttcag gagtacccag agttggtgtc ccctggcggg tgtgcctata caacaaggcg
     1381 ctacaacatg tgtgtgaggg acatgaacta cttcctcagg atgtgtagct acgccatcgt
     1441 ggccgggggc gccagcgtct tggatggaag gatgctggcc gggttccggg atacattcaa
     1501 ctccctcggc atccctctgt gccccgctgc ccggggcatc caactgatga agaagatcgt
     1561 caaagagaaa ctggcgacag ccggcatgac taacattgca tttgtcgatg agccctttga
     1621 ctacatcgcc cgtgtgatta gcgaaacgga aatttaagaa ttcgatatca agcttatcga
     1681 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1741 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1801 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     1861 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     1921 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     1981 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2041 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2101 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2161 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2221 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     2281 tcgacccggg cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     2341 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2401 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2461 gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2521 tggtaaaatc gataaggatc tcccctagag catggctacg tagataagta gcatggcggg
     2581 ttaatcatta actacaagga acccctagtg atggagttgg catcctctct tttgggcggg
     2641 tctcc
//
