LOCUS       sequence-383311-        3119 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the protein of emiRFP670 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, 
            Koster RW, Wu L, Namikawa K, Piatkevich KD
  TITLE     Quantitative assessment of near-infrared fluorescent proteins.
  JOURNAL   Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z.
  PUBMED    37666982
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z."
COMMENT     Sequence Label: pAAV-CAG-emiRFP670
FEATURES             Location/Qualifiers
     source          1..3119
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        495..772
                     /label=chicken beta-actin promoter
     primer_bind     1051..1073
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1135..1154
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1193..1202
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2156..2744
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2209..2229)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2627..2638)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2787..2908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2798..2817)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2852..2871
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cagtaccatg acgtcaggcg gccatcgtgg tacgccgatc cgtgccggtc gctgattatt
       61 atgcagctgc ccgctcgctc gctcactgag ccgccgggca aagcccgggc gtcgggcgac
      121 tttgttcgcc cgccctcagt gaccgaccga accgccccag agaggagtgc actcatcact
      181 agggtctgta gtaatgatta acccgccatg ctactatcta cgtagccatg ctctaggaag
      241 agtaccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      301 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      361 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      421 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      481 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
      541 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
      601 gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
      661 gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
      721 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc
      781 tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
      841 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      901 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
      961 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg cggggggacg
     1021 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     1081 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     1141 tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatccggta ccgccaccat
     1201 ggccgagggc agcgtggcca gacagcccga cctgctgacc tgcgaacatg aagagatcca
     1261 cctcgccggc tcgatccagc cgcatggcgc gcttctggtc gtcagcgaac atgatcatcg
     1321 cgtcatccag gccagcgcca acgccgcgga atttctgaat ctcggaagcg tactcggcgt
     1381 tccgctcgcc gagatcgacg gcgatctgtt gatcaagatc ctgccgcatc tcgatcccac
     1441 cgccgaaggc atgccggtcg cggtgcgctg ccggatcggc aatccctcta cggagtactg
     1501 cggtctgatg catcggcctc cggaaggcgg gctgatcatc gaactcgaac gtgccggccc
     1561 gtcgatcgat ctgtcaggca cgctggcgcc ggcgctggag cggatccgca cggcgggttc
     1621 actgcgcgcg ctgtgcgatg acaccgtgct gctgtttcag cagtgcaccg gctacgaccg
     1681 ggtgatggtg tatcgtttcg atgagcaagg ccacggcctg gtattctccg agtgccatgt
     1741 gcctgggctc gaatcctatt tcggcaaccg ctatccgtcg tcgactgtcc cgcagatggc
     1801 gcggcagctg tacgtgcggc agcgcgtccg cgtgctggtc gacgtcacct atcagccggt
     1861 gccgctggag ccgcggctgt cgccgctgac cgggcgcgat ctcgacatgt cgggctgctt
     1921 cctgcgctcg atgtcgccgt gccatctgca gttcctgaag gacatgggcg tgcgcgccac
     1981 cctggcggtg tcgctggtgg tcggcggcaa gctgtggggc ctggttgtct gtcaccatta
     2041 tctgccgcgc ttcatccgtt tcgagctgcg ggcgatctgc aaacggctcg ccgaaaggat
     2101 cgcgacgcgg atcaccgcgc ttgagagcta agaattcgat atcaagctta tcgataatca
     2161 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2221 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2281 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2341 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2401 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2461 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2521 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     2581 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2641 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2701 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     2761 cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
     2821 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2881 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     2941 ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     3001 aatcgataag gatctcccct agagcatggc tacgtagata agtagcatgg cgggttaatc
     3061 attaactaca aggaacccct agtgatggag ttggcatcct ctcttttggg cgggtctcc
//
