LOCUS       sequence-38333-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Pereira MJ, Behera V, Walter NG
  TITLE     Nondenaturing purification of co-transcriptionally folded RNA avoids
            common folding heterogeneity.
  JOURNAL   PLoS One. 2010  . 5(9):e12953.
  PUBMED    20886091
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(9):e12953."
COMMENT     Sequence Label: pMCGL
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(244..262)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(245..262)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(270..287)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(270..286)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(279..301)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(495..514)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     614..636
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(674..692)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        760..864
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcctctaga gtcgacctgc aggcatgcgc gcccgaactt aagcagcgag gagaatcatg
       61 caagtgcgta agatagtcgc gccccgcggt ttaagttgac gaggatggag gttatcgaat
      121 tttcggcgga tgcctcccgg ctgagtgtgc agatcacagc cgtaaggatt tcttcaaacc
      181 aagggggtga ctccttgaac aaagagaaat cacatgatcc gagcaggcct gcggaattcg
      241 tattctatag tgtcacctaa atccaattca ctggccgtcg ttttacaacg tcgtgactgg
      301 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      361 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      421 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
      481 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
      541 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
      601 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
      661 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
      721 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      781 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      841 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      901 ttt
//
