LOCUS       sequence-38334-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Kwon ES, Narasimhan SD, Yen K, Tissenbaum HA
  TITLE     A new DAF-16 isoform regulates longevity.
  JOURNAL   Nature. 2010 Jul 22. 466(7305):498-502.
  PUBMED    20613724
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 22. 466(7305):498-502."
COMMENT     Sequence Label: L4440-daf-16b
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(511..527)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(512..528)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(552..571)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(553..571)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(581..598)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(581..597)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(590..612)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(826..845)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtgttacacg tggccaatgc aacaatacat ctatcaggaa tcgtcagcaa ccattcccca
       61 tcaccattta aatcaacaca acaatccgta tcatccaatg catcctcatc atcaattacc
      121 tcatatgcaa caacttcctc aacctctatt gaatcttaac atgacgacgt taacatcttc
      181 tggcagttcc gtggccagtt ccattggagg cggagctcaa tgctctccgt gcgcgtcggg
      241 ctcctcgacc gctgcaacaa attcctctca acagcagcag accgttggtc aaatgcttgc
      301 tgcatcggtg ccttgttctt catctggcat gacacttgga atgtcactta atctgtcaca
      361 aggcggtggt ccaatgccgg caaaaaagaa gcgttgtcgt aagaagccaa ccgatcaatt
      421 ggcacagagg aaaccgaatc catggggtga ggaatcctat tcggatatca ttgccaaagc
      481 attggaatcg gcgccagacg gaaagcttat cgataccgtc gacctcgagg gggggcccgg
      541 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      601 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      661 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      721 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      781 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      841 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      901 ctttaggg
//
