LOCUS       sequence-38335-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Zuniga JE, Schmidt JJ, Fenn T, Burnett JC, Arac D, Gussio R, 
            Stafford RG, Badie SS, Bavari S, Brunger AT
  TITLE     A potent peptidomimetic inhibitor of botulinum neurotoxin serotype A
            has a very different conformation than SNAP-25 substrate.
  JOURNAL   Structure. 2008 Oct 8. 16(10):1588-97.
  PUBMED    18940613
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Structure. 
            2008 Oct 8. 16(10):1588-97."
COMMENT     Sequence Label: BoNT/A-LC
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      193..287
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(397..416)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtaattcaca atatatgatg gatttaattt aagaaataca aatttagcag caaactttaa
       61 tggtcaaaat acagaaatta ataatatgaa ttttactaaa ctaaaaaatt ttactcccct
      121 ggttccgcgt ggatctgggc atcaccatca ccatcactaa ggcctgcagc caagcttaat
      181 tagctgagct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
      241 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagcttg
      301 gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
      361 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
      421 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
      481 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
      541 ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
      601 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
      661 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
      721 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
      781 gtgagtttca
//
