LOCUS       sequence-38338-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Kwon ES, Narasimhan SD, Yen K, Tissenbaum HA
  TITLE     A new DAF-16 isoform regulates longevity.
  JOURNAL   Nature. 2010 Jul 22. 466(7305):498-502.
  PUBMED    20613724
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jul 22. 466(7305):498-502."
COMMENT     Sequence Label: L4440-daf-16df
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(227..243)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(228..244)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(268..287)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(269..287)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(297..314)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(297..313)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(306..328)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(542..561)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     752..773
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtctctaaag atgtgtggaa atacggagag aacgagcatt tttcgcgaag aaattgttga
       61 gaatactggg ttactccgcc aaaagaagcc gaacgctctt gttgatggag gtcgagattg
      121 aagaaatcat cgtcaaaatc agtggaaaaa ctcattccgg aatcaggcct tgccggttcg
      181 agattgtgca gtgtgatgtc agatggtagc ggcgaatcaa gcttatcgat accgtcgacc
      241 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
      301 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      361 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      421 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
      481 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      541 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      601 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      661 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      721 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
      781 c
//
