LOCUS       sequence-38348-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Hung YP, Albeck JG, Tantama M, Yellen G
  TITLE     Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded
            Fluorescent Biosensor.
  JOURNAL   Cell Metab. 2011 Oct 5;14(4):545-54.
  PUBMED    21982714
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Metab."; date: "2011-10-5"; volume: "14(4)"; pages: "545-54"
COMMENT     Sequence Label: GW1-Peredox-mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             244..264
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    398..532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(435..454)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     489..508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(555..571)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(555..571)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(568..590)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    579..595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(603..609,610..627,628..633))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(785..802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
       61 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      121 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      181 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgaactgtac
      241 aagcctaaaa agaaacgtaa agttgaagat gcttgagtcg acgaattcat tgatcataat
      301 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      361 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      421 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      481 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgtaccga
      541 gctcgcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      601 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      661 ctacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      721 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      781 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      841 actcaaaggc ggtaatacgg ttatccacag aatcaggg
//
