LOCUS       sequence-38353-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   He A, Kong SW, Ma Q, Pu WT
  TITLE     Co-occupancy by multiple cardiac transcription factors identifies 
            transcriptional enhancers active in heart.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Apr 5. 108(14):5632-7.
  PUBMED    21415370
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Apr 5. 108(14):5632-7."
COMMENT     Sequence Label: pENTR-flbio
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..71
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             122..145
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             158..202
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     protein_bind    complement(247..346)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(458..477)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(536..555)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtacaa
       61 aaaagcaggc tctttaaagg aaccaattca gtcgactgga tccggtaccg aattcactag
      121 tgactacaaa gacgatgacg ataaaggtga tgggtccggc ctgaacgaca tcttcgaggc
      181 tcagaaaatc gaatggcacg aaggcgcgcc gagctcgagg taggcggccg cactcgagat
      241 atctagaccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
      301 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag
      361 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
      421 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
      481 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      541 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
      601 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      661 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      721 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
      781 ttccaggtat tagaagaata tcctg
//
