LOCUS       sequence-38363-e        8158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Zhu J, Smith K, Hsieh PN, Mburu YK, Chattopadhyay S, Sen GC, Sarkar 
            SN
  TITLE     High-throughput screening for TLR3-IFN regulatory factor 3 signaling
            pathway modulators identifies several antipsychotic drugs as TLR 
            inhibitors.
  JOURNAL   J Immunol. 2010 May 15;184(10):5768-76. Epub 2010 Apr 9.
  PUBMED    20382888
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2010-05-15"; volume: "184(10)"; pages: "5768-76. 
            Epub 2010 Apr 9"
COMMENT     Sequence Label: hTLR3-pcDNA3
FEATURES             Location/Qualifiers
     source          1..8158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             52..96
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(2815..2833)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2816..2833)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2853..2870)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2859..3083
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3129..3557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3216..3235)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3426..3447
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3566..3586)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3571..3901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3752..3887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3814..3833
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3968..4762
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4022..4041)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4632..4651
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4936..5057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4973..4992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5027..5046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5106..5122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5106..5122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5119..5141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5130..5146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5154..5160,5161..5178,5179..5184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5199..5220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5337..5354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5508..6096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5588..5607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6267..7058,7059..7127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6890..6909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7128..7232)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7307..7326)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7498..7877
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7878..8081
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8032..8052
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8126..8145
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8126..8144
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcac catgtctgca
       61 cttctgatcc tagctcttgt tggagctgca gttgctgact acaaagacga tgacgacaag
      121 cttgggatgc tgtgtgcatc ctccaccacc aagtgcactg ttagccatga agttgctgac
      181 tgcagccacc tgaagttgac tcaggtaccc gatgatctac ccacaaacat aacagtgttg
      241 aaccttaccc ataatcaact cagaagatta ccagccgcca acttcacaag gtatagccag
      301 ctaactagct tggatgtagg atttaacacc atctcaaaac tggagccaga attgtgccag
      361 aaacttccca tgttaaaagt tttgaacctc cagcacaatg agctatctca actttctgat
      421 aaaacctttg ccttctgcac gaatttgact gaactccatc tcatgtccaa ctcaatccag
      481 aaaattaaaa ataatccctt tgtcaagcag aagaatttaa tcacattaga tctgtctcat
      541 aatggcttgt catctacaaa attaggaact caggttcagc tggaaaatct ccaagagctt
      601 ctattatcaa acaataaaat tcaagcgcta aaaagtgaag aactggatat ctttgccaat
      661 tcatctttaa aaaaattaga gttgtcatcg aatcaaatta aagagttttc tccagggtgt
      721 tttcacgcaa ttggaagatt atttggcctc tttctgaaca atgtccagct gggtcccagc
      781 cttacagaga agctatgttt ggaattagca aacacaagca ttcggaatct gtctctgagt
      841 aacagccagc tgtccaccac cagcaataca actttcttgg gactaaagtg gacaaatctc
      901 actatgctcg atctttccta caacaactta aatgtggttg gtaacgattc ctttgcttgg
      961 cttccacaac tagaatattt cttcctagag tataataata tacagcattt gttttctcac
     1021 tctttgcacg ggcttttcaa tgtgaggtac ctgaatttga aacggtcttt tactaaacaa
     1081 agtatttccc ttgcctcact ccccaagatt gatgattttt cttttcagtg gctaaaatgt
     1141 ttggagcacc ttaacatgga agataatgat attccaggca taaaaagcaa tatgttcaca
     1201 ggattgataa acctgaaata cttaagtcta tccaactcct ttacaagttt gcgaactttg
     1261 acaaatgaaa catttgtatc acttgctcat tctcccttac acatactcaa cctaaccaag
     1321 aataaaatct caaaaataga gagtgatgct ttctcttggt tgggccacct agaagtactt
     1381 gacctgggcc ttaatgaaat tgggcaagaa ctcacaggcc aggaatggag aggtctagaa
     1441 aatattttcg aaatctatct ttcctacaac aagtacctgc agctgactag gaactccttt
     1501 gccttggtcc caagccttca acgactgatg ctccgaaggg tggcccttaa aaatgtggat
     1561 agctctcctt caccattcca gcctcttcgt aacttgacca ttctggatct aagcaacaac
     1621 aacatagcca acataaatga tgacatgttg gagggtcttg agaaactaga aattctcgat
     1681 ttgcagcata acaacttagc acggctctgg aaacacgcaa accctggtgg tcccatttat
     1741 ttcctaaagg gtctgtctca cctccacatc cttaacttgg agtccaacgg ctttgacgag
     1801 atcccagttg aggtcttcaa ggatttattt gaactaaaga tcatcgattt aggattgaat
     1861 aatttaaaca cacttccagc atctgtcttt aataatcagg tgtctctaaa gtcattgaac
     1921 cttcagaaga atctcataac atccgttgag aagaaggttt tcgggccagc tttcaggaac
     1981 ctgactgagt tagatatgcg ctttaatccc tttgattgca cgtgtgaaag tattgcctgg
     2041 tttgttaatt ggattaacga gacccatacc aacatccctg agctgtcaag ccactacctt
     2101 tgcaacactc cacctcacta tcatgggttc ccagtgagac tttttgatac atcatcttgc
     2161 aaagacagtg ccccctttga actctttttc atgatcaata ccagtatcct gttgattttt
     2221 atctttattg tacttctcat ccactttgag ggctggagga tatcttttta ttggaatgtt
     2281 tcagtacatc gagttcttgg tttcaaagaa atagacagac agacagaaca gtttgaatat
     2341 gcagcatata taattcatgc ctataaagat aaggattggg tctgggaaca tttctcttca
     2401 atggaaaagg aagaccaatc tctcaaattt tgtctggaag aaagggactt tgaggcgggt
     2461 gtttttgaac tagaagcaat tgttaacagc atcaaaagaa gcagaaaaat tatttttgtt
     2521 ataacacacc atctattaaa agacccatta tgcaaaagat tcaaggtaca tcatgcagtt
     2581 caacaagcta ttgaacaaaa tctggattcc attatattgg ttttccttga ggagattcca
     2641 gattataaac tgaaccatgc actctgtttg cgaagaggaa tgtttaaatc tcactgcatc
     2701 ttgaactggc cagttcagaa agaacggata ggtgcctttc gtcataaatt gcaagtagca
     2761 cttggatcca aaaactctgt acattaactc gagcatgcat ctagagggcc ctattctata
     2821 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     2881 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2941 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3001 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3061 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     3121 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3181 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3241 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
     3301 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3361 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3421 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3481 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
     3541 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     3601 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3721 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3841 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3901 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     3961 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4021 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4081 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4141 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4201 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4261 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4321 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4381 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4441 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     4501 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4561 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4621 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4681 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4741 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4801 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4861 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4921 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4981 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5041 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     5101 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5161 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5221 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5281 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5401 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5461 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5701 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     5941 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7261 gtcgacggat cgggagatct cccgatcccc tatggtcgac tctcagtaca atctgctctg
     7321 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     7381 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     7441 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     7501 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     7561 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     7621 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     7681 tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca gtacatcaag
     7741 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     7801 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     7861 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     7921 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     7981 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     8041 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     8101 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagcttg
//
