LOCUS       sequence-383646-        9631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with IAA17 
            (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology 
            arms for genome integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 9631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT IAA17
FEATURES             Location/Qualifiers
     source          1..9631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2464..2486
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2478..2495
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2479..2495
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2505..2524
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2505..2523
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2548..2564
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2549..2565
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2659..3342
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     promoter        3795..4196
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4218..4238
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4776..4802
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4812..4868
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4905..4925
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4941..6662
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(6857..6877)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6857..6875)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6896..6912)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6896..6912)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6909..6931)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6920..6936
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6944..6950,6951..6968,6969..6974))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6989..7010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7127..7144)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7298..7886)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7378..7397)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8057..8848,8849..8917))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8680..8699
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8918..9022)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9059..9562
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9604..9622
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc
     2341 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     2401 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     2461 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     2521 agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
     2581 aattcctgca gctatattac cctgttatcc ctacgtacgc tgcaggtcga cggtggcggt
     2641 tctggcggtg gcggatccgg ctctgttgag ttaaacctgc gtgagaccga gctatgtctt
     2701 ggtctacccg gtggtgacac tgtagcacca gtgacaggca acaaaagggg cttcagcgag
     2761 actgtagatc tgaagctaaa cttaaacaac gaaccagcta ataaggaagg tagtacaaca
     2821 catgatgtgg ttacgtttga cagcaaggag aagagtgcct gtcccaaaga tccggcaaag
     2881 cccccggcga aggcccaggt tgtaggttgg cctccggtaa ggtcctatcg taaaaacgta
     2941 atggttagtt gtcagaagtc cagcggagga cctgaagcag cggcgttcgt caaagtatct
     3001 atggatggcg ccccatactt gagaaagata gacctgcgta tgtataagtc ttacgacgag
     3061 ttatcaaatg cgctatctaa catgttcagc agtttcacca tgggcaaaca tggtggtgag
     3121 gagggaatga tcgacttcat gaacgagaga aaacttatgg acctggtgaa tagctgggat
     3181 tatgtcccta gctacgagga taaagacggg gattggatgc ttgtggggga tgttccgtgg
     3241 ccgatgttcg tcgatacgtg taagcgtctg cgtctaatga agggctccga tgctattggt
     3301 ttagctccgc gtgctatgga aaagtgtaag tccagagctt agagttcttc tttgagatat
     3361 cgattgaacg agctcgaatt catcgatcta tattaccctg ttatccctag cgtaactatg
     3421 ccaaatgaca ataagacacc caataggtcc agcactccca agtttactaa aaaaccagta
     3481 accccgaatg ataaaattcc tgaaagacgg gtgtacaata tggacttcct cttttctggc
     3541 aaccaaaccc atacatcggg attcctataa taccttcgtt ggtctcccta acatgtaggt
     3601 ggcggagggg agatatacaa tagaacagat accagacaag acataatggg ctaaacaaga
     3661 ctacaccaat tacactgcct cattgatggt ggtacataac gaactaatac tgtagcccta
     3721 gacttgatag ccatcatcat atcgaagttt cactaccctt tttccatttg ccatctattg
     3781 aagtaataat aggcgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg
     3841 ttgtctcacc atatccgcaa tgacaaaaaa atgatggaag acactaaagg aaaaaattaa
     3901 cgacaaagac agcaccaaca gatgtcgttg ttccagagct gatgaggggt atctcgaagc
     3961 acacgaaact ttttccttcc ttcattcacg cacactactc tctaatgagc aacggtatac
     4021 ggccttcctt ccagttactt gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag
     4081 tataaataga cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc
     4141 ttccttgttt ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactatct
     4201 catatacaac tagaatgccc aagaagaaac gtaaggtggg ttccaccctt acgagcggtc
     4261 aacgtgacca cagagacgtt gaaaatgagt tatccagtgc agcttttagc agtcaaagtt
     4321 tcggggttcc agatatgtca ttcaaaccgg ggtgctccag tgacgtcgca gtcactgatg
     4381 cgggtatgcc tagccaaggg ttatggaaca accagacgca aaggatgcgt accttcacta
     4441 aggtccagaa aaggggctct gttgggagga gtatcgacat caccaggtat cgtgattacg
     4501 atgagctacg tcatgatctg gcatgtatgt tcggcataca ggggcagtta gaagaccctt
     4561 atcgtatgga ttggaagtta gtttatgtgg atcacgagaa cgatatactg ttggtgggcg
     4621 acgacccgtg ggaggaattt gtaggttgtg ttaagtctat caagattcta tctgctgcag
     4681 aggtgcagca aatgagcttg gacggggatc ttggtggagt tccgccccaa acccaggcgt
     4741 gcagtgcttc agacgatgcg aacgcttgga gaggctatcc ctacgacgtg cctgattatg
     4801 cgggtagcgg ggccactaac ttcagcttgt tgaagcaggc tggcgatgtg gaggagaatc
     4861 caggtccggc ggctaccgat aaagcggaat taattcccga gcctccaaaa aagaagagaa
     4921 aggtcgaatt gggtaccgcc acgtacttcc cggaggaggt ggtggagcac atcttcagct
     4981 tcctgccggc gcagcgcgac cgcaacacgg tctcgctcgt ctgcaaggtg tggtacgaga
     5041 tcgagaggct gagccgccgc ggcgtcttcg tgggcaactg ctacgccgtg cgcgccggcc
     5101 gcgtcgccgc gcggttcccc aacgtgcggg cgctcacggt gaaggggaag ccccacttcg
     5161 ccgacttcaa cctcgtgccc cccgactggg gcggctacgc ggggccgtgg atcgaggcgg
     5221 ccgcgagggg atgccacggc ctggaggagc tcaggatgaa gcggatggtg gtgtccgacg
     5281 agagcctcga gctgctggct cgctcgttcc cgcggttcag ggctcttgtt cttatcagct
     5341 gcgaggggtt cagcactgac gggctagccg ccgtcgcgag ccattgcaag cttctgaggg
     5401 agttggattt gcaggaaaat gaagtggagg atcgagggcc taggtggctt tcctgcttcc
     5461 ctgattcctg cacatcactt gtctcattga attttgcctg catcaaaggg gaggttaatg
     5521 ctggttcact ggagagactt gttagcaggt ccccaaacct gcggagtttg aggctgaatc
     5581 gatctgtatc ggtagataca cttgcaaaga tactactgcg tacccctaac ttggaggatt
     5641 tggggacagg gaatttgaca gatgacttcc aaactgagtc ctactttaag cttaccagtg
     5701 ctctggagaa atgcaagatg ttgaggagtt tgtctggatt ctgggatgct tctcctgttt
     5761 gcctgtcatt tatctacccc ctgtgtgctc aactgacagg attgaacttg agctatgcac
     5821 ccacacttga tgcttctgac cttacaaaaa tgattagccg ctgtgtgaag ctccaacgcc
     5881 tttgggtact ggattgtatc tcggacaaag gcttgcaagt ggtggcctcc agttgcaaag
     5941 acttgcaaga actcagggta tttccatcag atttctacgt agctggttat tctgcagtga
     6001 cagaggaggg acttgttgca gtatccttgg gctgtccaaa actgaactca ctactgtact
     6061 tctgtcacca aatgactaat gctgcactag ttactgtcgc caagaactgt ccaaatttca
     6121 cacgattcag actttgtatt cttgagccag ggaagcctga tgttgtgaca agccaaccat
     6181 tagatgaagg ctttggagct attgttcgtg agtgcaaggg attacaacgt ttgtcaatat
     6241 ctggtcttct cacagacaaa gttttcatgt atattgggaa atatgcaaaa caacttgaga
     6301 tgctttctat agcatttgct ggtgacagtg ataagggtat gatgcatgtt atgaatggat
     6361 gcaagaattt aaggaaactg gagataagag atagcccgtt tggtgatgct gcactcttgg
     6421 ggaattttgc taggtacgag acaatgcgat ccctttggat gtcatcttgc aatgtcacgt
     6481 taaaggggtg ccaagtcctt gcgtcaaaga tgccgatgct caatgttgag gtcataaatg
     6541 agcgggatgg tagcaatgaa atggaggaaa accatggaga tctgcctaaa gtggagaaat
     6601 tatatgtgta ccgcacaact gctggggcga gggatgatgc accaaatttt gttaaaatcc
     6661 tagtcgactc cggttctgct gctagtggtt gaacgcgtgg cgcctggaaa aagattttca
     6721 agtgaataag gagataaaac cgtattgact ttttatcatt ctacaaaaga ccagtaacga
     6781 agtgtaccct gctatattac cctgttatcc ctagcgtaac tcggccgcca ccgcggtgga
     6841 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     6901 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6961 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7021 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7081 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7141 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7201 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7261 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7321 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7381 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7441 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7501 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7561 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7621 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7681 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7741 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7801 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7861 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7921 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7981 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8041 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8101 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8161 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8221 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8281 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8341 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8401 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8461 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8521 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8581 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8641 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8701 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8761 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8821 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8881 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8941 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9001 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg tccttttcat
     9061 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat
     9121 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac
     9181 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta
     9241 atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta
     9301 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa
     9361 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct
     9421 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa
     9481 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg
     9541 tgtaagttac aggcaagcga tccgtcctaa gaaaccatta ttatcatgac attaacctat
     9601 aaaaataggc gtatcacgag gccctttcgt c
//
