LOCUS       sequence-383663-       10861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations
            followed by an internal ribosomal entry site followed by EGFP under 
            the human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
  TITLE     Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in 
            Striatal Neurons by Regulating Substance P Secretion.
  JOURNAL   J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 
            10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2.
  PUBMED    36732068
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.1857-22.2023"; journalName: "J Neurosci"; date: 
            "2023-03-1- 1"; volume: "43"; issue: "9"; pages: "1475-1491"
COMMENT     Sequence Label: pFSynW SYT9 D197, 199, 330, 332N IRES GFP
FEATURES             Location/Qualifiers
     source          1..10861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    4237..4808
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4258..4810
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4402..4419)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4629..4648
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4810..4812,4813..4815,4816..5529)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4810..4812,4813..4815,4816..5526)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4855..4876)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5116..5135)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5463..5484
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5553..6141
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5606..5626)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6024..6035)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6144..6160)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6145..6161)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6666..6846
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6872..6889)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6878..7102
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7148..7576
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7235..7254)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7445..7466
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7585..7605)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7590..7919
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7770..7905
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7832..7851
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7967..8014
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8033..8407
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8537..8658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8574..8593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8628..8647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8707..8723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8707..8723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8720..8742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8731..8747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8755..8761,8762..8779,8780..8785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8800..8821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8938..8955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9109..9697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9189..9208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9868..10659,10660..10728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10491..10510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10729..10833)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac tgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 gatccatgtt cccggaaccc ccgaccctgg ggtctccagc gcctaagaca cctccagact
     3121 ccagccgcat caggcagggc gcagtgcctg cctgggtcct ggctaccatc gtgctgggct
     3181 cgggcctcct ggtcttcagc agttgtttct gtctctaccg gaagcgctgt cggagacgga
     3241 tgggcaaaaa gagtcaggcc caagcccaag tccatcttca ggaagtgaag gagctgggcc
     3301 ggagttacat agataaggtt cagcctgaaa tagaggagct ggaccggtca ccatccatgc
     3361 ctggacagca ggtatcagac aaacaccagc taggccgact gcagtattca ctggactatg
     3421 acttccagac tggccagctc ctggtgggca tcttgcaagc tcagggactg gctgcgttgg
     3481 acctaggagg ttcctcagat ccctatgtta gtgtctatct gctgccagac aagcggaggc
     3541 gacatgagac caaggtgcat cggcagaccc tgaatccaca ctttggagag acatttgcct
     3601 tcaaggtccc atacgtggaa ctagggggca gagtgctggt catggcggtg tataacttta
     3661 atcggttctc ccgcaacgat gccatcgggg aggtgcgggt gcctatgagt tcagtgaacc
     3721 tggggcggcc ggtgcaggcc tggagagagc tgcaggtggc tcccaaagag gagcaagaga
     3781 aactggggga catctgcttc tctctccgtt atgtccccac ggccgggaag ctcaccgtca
     3841 ttgtcttgga agctaaaaac ctgaagaaaa tggatgtagg aggactctca gatccctatg
     3901 tgaaggtgca cctgctccag ggaggtaaaa aggttcgcaa gaagaaaacc accattaaga
     3961 agaacaccct gaacccctat tacaacgagg ccttcagctt tgaggtgccc tgtgaccagg
     4021 tgcagaaagt ccaggtggag ctgactgttc tgaattataa caaactgggg aagaatgagg
     4081 ccatcgggag agtggcggtg ggcgcagcag tcggtggggc tggcctgcgg cactgggctg
     4141 acatgttggc caaccccagg cgccccattg cccagtggca ctctctgcgg ccccctgaca
     4201 gagccaggcc aataccagca ccctaatcta gaattccccc tctccctccc ccccctaacg
     4261 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4321 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4381 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4441 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4501 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4561 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4621 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4681 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
     4741 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     4801 gatgataata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4861 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4921 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     4981 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     5041 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     5101 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     5161 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     5221 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     5281 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     5341 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     5401 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     5461 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5521 tacaagtaaa attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     5581 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5641 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5701 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5761 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5821 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5881 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5941 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6001 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6061 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6121 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag
     6181 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     6241 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     6301 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6361 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     6421 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
     6481 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
     6541 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
     6601 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
     6661 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6721 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6781 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6841 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     6901 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6961 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     7021 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     7081 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     7141 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7201 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7261 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7321 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7381 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7441 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7501 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7561 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     7621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     7681 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7741 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7801 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     7861 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7921 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     7981 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     8041 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     8101 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     8161 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     8221 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     8281 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     8341 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     8401 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     8461 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     8521 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8581 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8641 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     8701 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8761 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8821 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8881 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8941 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9001 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9061 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9121 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9181 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9241 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9301 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9361 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9421 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9481 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9541 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9601 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9661 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9721 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9781 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9841 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9901 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9961 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10021 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10081 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10141 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10201 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10261 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10321 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10381 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10441 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10501 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10561 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10621 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10681 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10741 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10801 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10861 c
//
