LOCUS       sequence-383665-       11694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag 
            followed by an internal ribosomal entry site followed by mRuby3 
            under the human synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
  TITLE     Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in 
            Striatal Neurons by Regulating Substance P Secretion.
  JOURNAL   J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 
            10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2.
  PUBMED    36732068
REFERENCE   2  (bases 1 to 11694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.1857-22.2023"; journalName: "J Neurosci"; date: 
            "2023-03-1- 1"; volume: "43"; issue: "9"; pages: "1475-1491"
COMMENT     Sequence Label: pFSynW pHluorin-SYT9 IRES mRuby3
FEATURES             Location/Qualifiers
     source          1..11694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3060..3069
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3066..3155
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             3174..3884
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3201..3229)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3824..3844
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    5094..5665
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5115..5667
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5259..5276)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5486..5505
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6386..6974
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6439..6459)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6857..6868)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6977..6993)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6978..6994)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7499..7679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7705..7722)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7711..7935
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7981..8409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8068..8087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8278..8299
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8418..8438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8423..8752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8603..8738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8665..8684
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8800..8847
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8866..9240
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9370..9491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9407..9426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9461..9480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9540..9556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9540..9556)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9553..9575)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9564..9580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9588..9594,9595..9612,9613..9618))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9633..9654
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9771..9788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9942..10530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10022..10041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10701..11492,11493..11561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11324..11343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11562..11666)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 ccaccatgga cagcaaaggt tcgtcgcaga aagggtcccg cctgctcctg ctgctggtgg
     3121 tgtcaaatct actcttgtgc cagggtgtgg tctccgatat cggtaccacg cgtagtaaag
     3181 gcgaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     3241 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     3301 ttaaatttat ttgcactact ggaaaactac ctgttccttg gccaacactt gtcactactt
     3361 taacttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacgg catgactttt
     3421 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     3481 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     3541 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     3601 actataacga tcaccaggtg tacatcatgg cagacaaaca aaagaatgga atcaaagcta
     3661 acttcaaaat tagacacaac attgaagatg gaggcgttca actagcagac cattatcaac
     3721 aaaatactcc aattggcgat gggcccgtcc ttttaccaga caaccattac ctgtttacaa
     3781 cttctactct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     3841 taacagctgc tgggattaca catggcatgg atgaactata caaagaattc tcaggcggaa
     3901 gcggaggctc cggcgggagc ggcggaatgt tcccggaacc cccgaccccg gggtctccag
     3961 cgcctgagac acctccagac tccagccgca tcaggcaggg ggcagtgcct gcctgggtcc
     4021 tggctaccat cttgctgggc tcaggcctcc tggtcttcag cagctgtttc tgtctctacc
     4081 ggaagcgctg tcggagacgg atgggcaaaa agagtcaggc ccaagcccaa gtccatcttc
     4141 aggaagtgaa ggagctgggc cggagttaca tagataaggt tcagcctgaa atagaggaac
     4201 tggacccctc accatccatg ccaggacagc aggtattgga caagcaccag ttaggccgac
     4261 tgcagtattc actggattat gacttccaga ctggccagct cctggtaggc atcttgcaag
     4321 ctgagggact ggcagcgttg gacctaggag gttcctcaga cccctatgtt agtgtctatc
     4381 tgctgccaga caagcggagg cgacatgaga ccaaggtgca tcggcagacc ctgaatccac
     4441 actttggaga gacctttgcc ttcaaggtcc catacgtgga actaggaggt agagtgctgg
     4501 tcatggcggt gtatgacttc gatcgcttct cccgcaacga tgccatcgga gaggtgcggg
     4561 tgcctatgag ttcagtgaac ctggggcggc cagtgcaggc ctggcgagag ctgcaggtgg
     4621 ctcccaaaga ggagcaggag aaactggggg atatctgctt ctctctccgg tatgtcccca
     4681 cggccgggaa gctcaccgtc attgtcctgg aagctaaaaa cctgaagaaa atggatgtag
     4741 gaggactctc agacccttat gtcaaggtgc acctgctcca gggaggtaaa aaggttcgga
     4801 agaagaaaac caccattaag aagaacaccc tgaaccccta ttacaacgag gccttcagct
     4861 ttgaggtgcc ctgtgaccag gtgcagaaag tccaggtgga gctgaccgtt ttggactatg
     4921 acaaactggg gaagaatgag gccatcggga gagtggcggt gggcacagca gttggtgggg
     4981 ctggcctacg gcactgggct gacatgttgg ccaaccctag gcggcccatt gcccagtggc
     5041 actctctgcg gccccctgac agagccaggc caatacctgc accctgagaa ttccccctct
     5101 ccctcccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     5161 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     5221 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     5281 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     5341 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     5401 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     5461 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     5521 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt
     5581 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     5641 tttcctttga aaaacacgat gataatatgg tgtctaaggg cgaagagctg atcaaggaaa
     5701 atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg ccaccaattc aaatgcacag
     5761 gtgaaggaga aggcagaccg tacgagggag tgcaaaccat gaggatcaaa gtcatcgagg
     5821 gaggacccct gccatttgcc tttgacattc ttgccacgtc gttcatgtat ggcagccgta
     5881 cctttatcaa gtacccggcc gacatccctg atttctttaa acagtccttt cctgagggtt
     5941 ttacttggga aagagttacg agatacgaag atggtggagt cgtcaccgtc acgcaggaca
     6001 ccagccttga ggatggcgag ctcgtctaca acgtcaaggt cagaggggta aactttccct
     6061 ccaatggtcc cgtgatgcag aagaagacca agggttggga gcctaataca gagatgatgt
     6121 atccagcaga tggtggtctg agaggataca ctgacatcgc actgaaagtt gatggtggtg
     6181 gccatctgca ctgcaacttc gtgacaactt acaggtcaaa aaagaccgtc gggaacatca
     6241 agatgcccgg tgtccatgcc gttgatcacc gcctggaaag gatcgaggag agtgacaatg
     6301 aaacctacgt agtgcaaaga gaagtggcag ttgccaaata cagcaacctt ggtggtggct
     6361 aaaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     6421 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6481 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6541 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6601 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6661 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6721 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6781 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6841 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6901 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6961 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     7021 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     7081 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     7141 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7201 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     7261 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     7321 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     7381 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     7441 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     7501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7681 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7741 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7801 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7861 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7921 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7981 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8041 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8101 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8161 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8221 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8281 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8341 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8401 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8761 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8821 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8881 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8941 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     9001 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     9061 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     9121 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     9181 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     9241 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     9301 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     9361 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9481 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9541 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9601 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9661 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9721 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9781 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9841 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcatggcgct ttctcatagc
    10141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10381 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10441 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10501 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10561 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10621 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10681 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10741 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10801 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10861 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10921 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10981 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11041 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11101 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11161 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11221 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11281 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11341 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11401 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11461 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11521 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11581 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11641 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
