LOCUS       sequence-383678-       10833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid encoding full-length mouse SYT9 followed by an 
            internal ribosomal entry site followed by mRuby under the human 
            synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
  TITLE     Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in 
            Striatal Neurons by Regulating Substance P Secretion.
  JOURNAL   J Neurosci. 2023 Mar 1;43(9):1475-1491. doi: 
            10.1523/JNEUROSCI.1857-22.2023. Epub 2023 Feb 2.
  PUBMED    36732068
REFERENCE   2  (bases 1 to 10833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.1857-22.2023"; journalName: "J Neurosci"; date: 
            "2023-03-1- 1"; volume: "43"; issue: "9"; pages: "1475-1491"
COMMENT     Sequence Label: pFSynW Syt9 IRES mRuby3
FEATURES             Location/Qualifiers
     source          1..10833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    4233..4804
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4254..4806
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4398..4415)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4625..4644
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5525..6113
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5578..5598)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5996..6007)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6116..6132)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6117..6133)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6638..6818
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6844..6861)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6850..7074
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7120..7548
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7207..7226)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7417..7438
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7557..7577)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7562..7891
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7742..7877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7804..7823
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7939..7986
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8005..8379
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8509..8630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8546..8565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8600..8619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8679..8695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8679..8695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8692..8714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8703..8719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8727..8733,8734..8751,8752..8757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8772..8793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8910..8927)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9081..9669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9161..9180)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9840..10631,10632..10700))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10463..10482
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10701..10805)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctagcg
     3061 ccaccatgtt cccggaaccc ccgaccccgg ggtctccagc gcctgagaca cctccagact
     3121 ccagccgcat caggcagggg gcagtgcctg cctgggtcct ggctaccatc ttgctgggct
     3181 caggcctcct ggtcttcagc agctgtttct gtctctaccg gaagcgctgt cggagacgga
     3241 tgggcaaaaa gagtcaggcc caagcccaag tccatcttca ggaagtgaag gagctgggcc
     3301 ggagttacat agataaggtt cagcctgaaa tagaggaact ggacccctca ccatccatgc
     3361 caggacagca ggtattggac aagcaccagt taggccgact gcagtattca ctggattatg
     3421 acttccagac tggccagctc ctggtaggca tcttgcaagc tgagggactg gcagcgttgg
     3481 acctaggagg ttcctcagac ccctatgtta gtgtctatct gctgccagac aagcggaggc
     3541 gacatgagac caaggtgcat cggcagaccc tgaatccaca ctttggagag acctttgcct
     3601 tcaaggtccc atacgtggaa ctaggaggta gagtgctggt catggcggtg tatgacttcg
     3661 atcgcttctc ccgcaacgat gccatcggag aggtgcgggt gcctatgagt tcagtgaacc
     3721 tggggcggcc agtgcaggcc tggcgagagc tgcaggtggc tcccaaagag gagcaggaga
     3781 aactggggga tatctgcttc tctctccggt atgtccccac ggccgggaag ctcaccgtca
     3841 ttgtcctgga agctaaaaac ctgaagaaaa tggatgtagg aggactctca gacccttatg
     3901 tcaaggtgca cctgctccag ggaggtaaaa aggttcggaa gaagaaaacc accattaaga
     3961 agaacaccct gaacccctat tacaacgagg ccttcagctt tgaggtgccc tgtgaccagg
     4021 tgcagaaagt ccaggtggag ctgaccgttt tggactatga caaactgggg aagaatgagg
     4081 ccatcgggag agtggcggtg ggcacagcag ttggtggggc tggcctacgg cactgggctg
     4141 acatgttggc caaccctagg cggcccattg cccagtggca ctctctgcgg ccccctgaca
     4201 gagccaggcc aatacctgca ccctgagaat tccccctctc cctccccccc ctaacgttac
     4261 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     4321 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     4381 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     4441 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     4501 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     4561 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     4621 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     4681 ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt
     4741 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     4801 ataatatggt gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca
     4861 tggaaggttc ggtcaacggc caccaattca aatgcacagg tgaaggagaa ggcagaccgt
     4921 acgagggagt gcaaaccatg aggatcaaag tcatcgaggg aggacccctg ccatttgcct
     4981 ttgacattct tgccacgtcg ttcatgtatg gcagccgtac ctttatcaag tacccggccg
     5041 acatccctga tttctttaaa cagtcctttc ctgagggttt tacttgggaa agagttacga
     5101 gatacgaaga tggtggagtc gtcaccgtca cgcaggacac cagccttgag gatggcgagc
     5161 tcgtctacaa cgtcaaggtc agaggggtaa actttccctc caatggtccc gtgatgcaga
     5221 agaagaccaa gggttgggag cctaatacag agatgatgta tccagcagat ggtggtctga
     5281 gaggatacac tgacatcgca ctgaaagttg atggtggtgg ccatctgcac tgcaacttcg
     5341 tgacaactta caggtcaaaa aagaccgtcg ggaacatcaa gatgcccggt gtccatgccg
     5401 ttgatcaccg cctggaaagg atcgaggaga gtgacaatga aacctacgta gtgcaaagag
     5461 aagtggcagt tgccaaatac agcaaccttg gtggtggcta aaattcgata tcaagcttat
     5521 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5581 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5641 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5701 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5761 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5821 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5881 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5941 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6001 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6061 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     6121 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     6181 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     6241 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6301 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     6361 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     6421 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     6481 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     6541 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     6601 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     6661 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6721 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6781 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     6841 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6901 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6961 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7021 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7081 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     7141 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7201 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7261 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7321 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7381 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7441 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7501 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     7561 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7621 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7681 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7741 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7801 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7861 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7921 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     7981 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     8041 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     8101 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     8161 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     8221 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     8281 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     8341 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     8401 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     8461 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     8521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8641 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8701 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8761 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8821 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8881 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8941 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9001 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9061 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9121 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9181 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9241 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9301 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9361 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9421 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9481 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9541 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9601 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9661 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9721 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9781 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9841 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9901 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9961 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10021 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10081 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10141 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10201 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10261 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10321 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10381 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10441 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10501 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10561 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10621 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10681 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10741 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10801 ccgcgcacat ttccccgaaa agtgccacct gac
//
