LOCUS       sequence-383679-         402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HMfA K31A E35A in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Erkelens AM, Henneman B, van der Valk RA, Kirolos NCS, Dame RT
  TITLE     Specific DNA binding of archaeal histones HMfA and HMfB.
  JOURNAL   Front Microbiol. 2023 Apr 18;14:1166608. doi: 
            10.3389/fmicb.2023.1166608. eCollection 2023.
  PUBMED    37143534
REFERENCE   2  (bases 1 to 402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2023-04-18"; volume: "14:1166608. doi"; pages: "
            10.3389/fmicb.2023.1166608. eCollection 2023"
COMMENT     Sequence Label: pRD323
FEATURES             Location/Qualifiers
     source          1..402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(341..359)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      355..402
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gggtgagtta ccaattgccc caatcggaag
      121 aatcataaaa aacgctggtg ctgaaagagt tagtgacgat gcaagaattg cattagcagc
      181 ggttttagaa gcgatgggcg aagaaatagc ttcagaagct gtgaaattag caaaacatgc
      241 gggaagaaaa acaataaagg cagaagacat tgaattagct cgaaaaatgt tcaaataact
      301 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      361 taaccccttg gggcctctaa acgggtcttg aggggttttt tg
//
