LOCUS       sequence-383695-        9163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-KIR2DS1-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        3105..3484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3485..3688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3639..3659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3685..3709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3919..3937
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3919..3936
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3950..3969
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4768..4803
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             4813..4836
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6496..6540
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6541..6567
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(6707..6724)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6713..6824
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6942..6962)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6947..7276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7127..7262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7189..7208
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7324..7371
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7390..7989
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7390..7409)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7886..7906
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8119..8240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8156..8175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8210..8229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8293..9084,9085..9153))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8916..8935
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9154..9163,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta ctgatgaatc ccctaatgat tttggtaaaa
     1741 atcattaagt taaggtggat acacatcttg tcatatgatc aaatggtttc gcgaaaaatc
     1801 aataatcaga caacaagatg tgcgaactcg atattttaca cgactctctt taccaattct
     1861 gccccgaatt acacttaaaa cgactcaaca gcttaacgtt ggcttgccac gcattacttg
     1921 actgtaaaac tctcactctt accgaacttg gccgtaacct gccaaccaaa gcgagaacaa
     1981 aacataacat caaacgaatc gaccgattgt taggtaatcg tcacctccac aaagagcgac
     2041 tcgctgtata ccgttggcat gctagcttta tctgttcggg caatacgatg cccattgtac
     2101 ttgttgactg gtctgatatt cgtgagcaaa aacgacttat ggtattgcga gcttcagtcg
     2161 cactacacgg tcgttctgtt actctttatg agaaagcgtt cccgctttca gagcaatgtt
     2221 caaagaaagc tcatgaccaa tttctagccg accttgcgag cattctaccg agtaacacca
     2281 caccgctcat tgtcagtgat gctggcttta aagtgccatg gtataaatcc gttgagaagc
     2341 tgggttggta ctggttaagt cgagtaagag gaaaagtaca atatgcagac ctaggagcgg
     2401 aaaactggaa acctatcagc aacttacatg atatgtcatc tagtcactca aagactttag
     2461 gctataagag gctgactaaa agcaatccaa tctcatgcca aattctattg tataaatctc
     2521 gctctaaagg ccgaaaaaat cagcgctcga cacggactca ttgtcaccac ccgtcaccta
     2581 aaatctactc agcgtcggca aaggagccat gggttctagc aactaactta cctgttgaaa
     2641 ttcgaacacc caaacaactt gttaatatct attcgaagcg aatgcagatt gaagaaacct
     2701 tccgagactt gaaaagtcct gcctacggac taggcctacg ccatagccga acgagcagct
     2761 cagagcgttt tgatatcatg ctgctaatcg ccctgatgct tcaactaaca tgttggcttg
     2821 cgggcgttca tgctcagaaa caaggttggg acaagcactt ccaggctaac acagtcagaa
     2881 atcgaaacgt actctcaaca gttcgcttag gcatggaagt tttgcggcat tctggctaca
     2941 caataacaag ggaagactta ctcgtggctg caaccctact agctcaaaat ttattcacac
     3001 atggttacgc tttggggaaa ttatgagggg atctctcagt acgttgtatc tatatcataa
     3061 tatgtacatt tatattggct catgtccaat atgaccgcca tgttggcatt gattattgac
     3121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     3721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
     3781 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
     3841 agtctatagg cccaccccct tggcttcgtt agaacgcggc tacaattaat acataacctt
     3901 atgtatcata cacatacgat ttaggtgaca ctatagaata acatccactt tgcctttctc
     3961 tccacaggtg tccactccca ggtccaactg cacctcggtt ctatcgaaaa cgcgcctgct
     4021 agcgccacca tgaagaccat cattgccctg agctacatct tctgcctggt gttcgctgcg
     4081 gccgcccatg aaggcgtgca cagaaaacct tctctgctgg cccatcctgg ccggctggtt
     4141 aagtctgaag agacagtgat cctgcagtgt tggagcgacg tgatgttcga gcacttcctg
     4201 ctgcaccgcg agggcatgtt caacgacaca ctgagactga tcggcgagca ccacgatggc
     4261 gtgtccaagg ccaatttcag catcagccgg atgagacagg atctggccgg cacctacaga
     4321 tgctacggct ctgtgacaca cagcccctat cagctgtctg cccctagcga tcccctggac
     4381 atcgtgatca tcggcctgta cgagaagcct tctctgagtg cccagcctgg acctacagtg
     4441 ctggctggcg aaaatgtgac cctgagctgt agcagccggt ccagctacga tatgtaccac
     4501 ctgagcagag agggcgaagc ccacgaacgt agactgcctg ccggcaccaa agtgaatggc
     4561 accttccagg ccaactttcc actgggacct gccacacacg gcggcacata tagatgcttt
     4621 ggcagcttcc gggacagccc ttacgagtgg tctaagagca gcgatcctct gctggtgtcc
     4681 gtgaccggca atcctagcaa tagctggcct tctccaaccg agcctagcag cgagacaggc
     4741 aaccccagac atctgcatgg cgcgccggaa gatcaagttg atccaagatt gattgatggc
     4801 aagggcggat ctctggaggt gctgttccag ggaccaggcg gaagcggcaa tcccggcggc
     4861 gatctggccc cccagatgct gcgcgagctg caggagacca atgccgccct gcaggacgtg
     4921 cgcgagctgc tgcgccaccg cgtgaaggag atcaccttcc tgaagaacac cgtgatggag
     4981 tgcgacgcct gcggtatgca gccctctaga ggctccggct ccatcatccc agttgaggag
     5041 gagaacccgg acttctggaa ccgcgaggca gccgaggccc tgggtgccgc caagaagctg
     5101 cagcctgcac agacagccgc caagaacctc atcatcttcc tgggcgatgg gatgggggtg
     5161 tctacggtga cagctgccag gatcctaaaa gggcagaaga aggacaaact ggggcctgag
     5221 atacccctgg ccatggaccg cttcccatat gtggctctgt ccaagacata caatgtagac
     5281 aaacatgtgc cagacagtgg agccacagcc acggcctacc tgtgcggggt caagggcaac
     5341 ttccagacca ttggcttgag tgcagccgcc cgctttaacc agtgcaacac gacacgcggc
     5401 aacgaggtca tctccgtgat gaatcgggcc aagaaagcag ggaagtcagt gggagtggta
     5461 accaccacac gagtgcagca cgcctcgcca gccggcacct acgcccacac ggtgaaccgc
     5521 aactggtact cggacgccga cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc
     5581 gctacgcagc tcatctccaa catggacatt gacgtgatcc taggtggagg ccgaaagtac
     5641 atgtttcgca tgggaacccc agaccctgag tacccagatg actacagcca aggtgggacc
     5701 aggctggacg ggaagaatct ggtgcaggaa tggctggcga agcgccaggg tgcccggtat
     5761 gtgtggaacc gcactgagct catgcaggct tccctggacc cgtctgtgac ccatctcatg
     5821 ggtctctttg agcctggaga catgaaatac gagatccacc gagactccac actggacccc
     5881 tccctgatgg agatgacaga ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc
     5941 ttcctcttcg tggagggtgg tcgcatcgac catggtcatc atgaaagcag ggcttaccgg
     6001 gcactgactg agacgatcat gttcgacgac gccattgaga gggcgggcca gctcaccagc
     6061 gaggaggaca cgctgagcct cgtcactgcc gaccactccc acgtcttctc cttcggaggc
     6121 taccccctgc gagggagctc catcttcggg ctggcccctg gcaaggcccg ggacaggaag
     6181 gcctacacgg tcctcctata cggaaacggt ccaggctatg tgctcaagga cggcgcccgg
     6241 ccggatgtta ccgagagcga gagcgggagc cccgagtatc ggcagcagtc agcagtgccc
     6301 ctggacgaag agacccacgc aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg
     6361 cacctggttc acggcgtgca ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc
     6421 tgcctggagc cctacaccgc ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg
     6481 cacccgggtg gcggaggact gaacgacatc ttcgaggctc agaaaatcga atggcacgaa
     6541 catcaccatc accatcacca tcaccattaa ggccggcctg tagtcactgg cacaacgcgt
     6601 tacccggtaa gccaatcggg tatacacggt cgtcatactg cagacagggt tcttctactt
     6661 tgcaagatag tcttgagtag taaaataaat agatagagaa aatcagcctc gactgtgcct
     6721 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6781 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcacaaca ctcaacccta
     6841 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6901 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     6961 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7021 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7081 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7201 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7261 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     7321 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     7381 aactaaacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     7441 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7501 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7561 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7621 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7681 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7741 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7801 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7861 gccttcctgg agacatccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7921 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7981 ggtgcctgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8041 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8101 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8221 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     8281 taatcatggt cattaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8341 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8401 accatctggc cccagcgctg cgatgatacc gcgagaacca cgctcaccgg ctccggattt
     8461 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8521 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8581 tagtttgcgc aacgttgttg ccatcgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8641 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8701 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8761 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8821 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8881 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8941 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9001 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9061 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9121 aataagggcg acacggaaat gttgaatact catattcttc ctt
//
