LOCUS       sequence-383701-       12013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 12013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SDK1(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..12013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             9355..9399
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9400..9417
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9557..9574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9563..9674
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9792..9812)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9797..10126
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9977..10112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10039..10058
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10174..10221
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10240..10839
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10240..10259)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10736..10756
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10969..11090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11006..11025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11060..11079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(11143..11934,11935..12003))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11766..11785
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12004..12013,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgg acctggtaga
     2761 cctagaggat ctcctccagg cagagccaga ccttctttgg ctcctagacc tggacctgag
     2821 cctagcagac caagagccgc tcctgaaaca tctggcggag atacagctgg cgctggaaga
     2881 tgtggtggaa gaagggccgc taaactcggc cctggtagaa gaggatggtg ggctctgctt
     2941 gccctgcagc ttcatctgct gagagccctg gctcaggatg acgtggcccc ttactttaag
     3001 acagagcccg gactgcccca gatccacctg gaaggcaata gactggtgct gacctgtctg
     3061 gccgaaggct cttggcctct ggaattcaag tggatgaggg acgacagcga gctgaccacc
     3121 tacagcagcg agtacaagta catcatcccc agcctgcaga agctggacgc cggcttttac
     3181 agatgcgtcg tgcggaatag aatgggcgcc ctgctgcaga gaaagtctga ggtgcaggtc
     3241 gcctacatgg gcagctttat ggacaccgac cagagaaaga ccgtgtctca gggcagagcc
     3301 gccatcctga atctgctgcc tatcacaagc taccccagac ctcaagtgac ctggttcaga
     3361 gagggccaca agatcatccc tagcaaccgg atcgccatca cactggaaaa ccagctcgtg
     3421 atcctggcca ccacaacatc tgatgctggc gcctattacg tgcaggccgt gaacgagaag
     3481 aacggcgaga acaagacaag ccccttcatc cacctgtcta tcgccagaga tgtgggcacc
     3541 cctgaaacaa tggcccctac aattgtggtg cctcctggca atagaagcgt ggtggccgga
     3601 agctctgaga caaccctgga atgtatcgcc agcgctagac ccgtggaaga tctgagcgtg
     3661 acctggaaga gaaacggcgt gcggatcaca tctggcctgc acagttttgg cagacggctg
     3721 accatcagca accccacaag tgccgataca ggcccttacg tgtgtgaagc tgctctgcca
     3781 ggatctgcct ttgagcctgc cagagccacc gcctttctgt tcatcatcga gcctccatac
     3841 ttcaccgccg agcctgagag cagaatcagc gccgaagtgg aagagacagt ggatatcggc
     3901 tgtcaggcca tgggagtgcc tctgcctact ctgcagtggt acaaggacgc catcagcatc
     3961 agcagactgc agaaccccag atacaaggtg ctggcttctg gcggcctgag aatccagaag
     4021 ctgaggcctg aggacagcgg catctttcag tgcttcgcct ctaacgaagg cggcgagatc
     4081 cagacacaca cctacctgga cgtgaccaat atcgcccctg tgttcaccca gaggcctgtg
     4141 gatacaaccg tgacagatgg catgaccgcc attctgagat gcgaagtgtc tggcgcccct
     4201 aagcctgcca tcacttggaa gagagagaac cacattctgg ccagcggctc cgtgcgcatc
     4261 cctagattca tgctgctgga aagcggcgga ctgcagattg ccccagtgtt tatccaggat
     4321 gccggcaact acacctgtta cgccgccaat accgagggca gcctgaatgc ctctgcaacc
     4381 ctgacagtgt ggaaccggac atccattgtg caccctccag aggaccacgt cgtgatcaag
     4441 ggaacaaccg ctacactgca ctgcggcgcc acacacgatc ctagagtgtc tctgagatac
     4501 gtgtggaaga aagacaacgt ggccctgaca cctagcagca ccagcagaat cgtggtggaa
     4561 aaagacggca gcctgctgat ctctcagact tggagcggcg acatcggcga ctactcttgc
     4621 gagattgtgt ctgaaggcgg caacgacagc cggatggcca gactggaagt gatcgaactg
     4681 cctcacagcc ctcagaacct gctggtgtct cctaatagca gccactctca cgccgtggtg
     4741 ctgagctggg ttcgaccttt tgacggcaac agccccatcc tgtactacat cgtggaactg
     4801 tccgagaaca acagcccttg gaaagtgcac ctgagcaacg tgggacctga gatgacaggc
     4861 gtgacagtgt ctggactgac ccctgccaga acctaccagt tcagagtgtg cgccgtgaat
     4921 gaagtcggca gaggccagta ctctgccgag acaagtagac tgatgctgcc cgaggaacct
     4981 cctagcgctc ctcctaagaa tatcgtggcc tccggcagga ccaaccagag cattatggtt
     5041 cagtggcagc ctccacctga gacagagcac aatggcgtgc tgagaggcta catcctgagg
     5101 tacagactgg ctggactgcc tggcgagtac cagcagagaa acatcacaag ccccgaagtg
     5161 aactactgcc tggtcaccga cctgatcatc tggacccagt atgagatcca ggtggccgcc
     5221 tataatggcg ccggactggg agtgtttagc agagccgtga ccgagtatac actgcagggc
     5281 gttccaaccg ctcctccaca gaatgtgcag accgaagccg tgaacagcac caccatccag
     5341 tttctgtgga accctccacc tcagcagttc atcaacggca tcaaccaggg ctataagctg
     5401 ctggcttggc cagccgatgc tcctgaagct gtgacagtgg tcacaatcgc cccagacttt
     5461 catggcgtgc accacggaca catcaccaac ctgaagaagt tcaccgccta cttcaccagc
     5521 gtgctgtgct ttacaacacc tggcgacggc cctccatcta cacctcagct tgtgtggacc
     5581 caagaggata agcctggcgc cgtgggacac ctgagcttta ccgagattct ggacaccagc
     5641 ctgaaggtgt cctggcaaga gcccctggaa aagaatggca tcatcaccgg ctaccagatc
     5701 agctgggaag tgtacggcag aaacgactcc agactgaccc acacactgaa tagcacaacc
     5761 cacgagtaca aaatccaggg cctgagcagc ctgaccacat acacaattga tgtggccgcc
     5821 gtgacagccg ttggcacagg actggtcaca agcagcacaa tctctagcgg cgtgccacca
     5881 gatttgcctg gcgctcctag caatctcgtg atcagcaaca tcagccccag aagcgccaca
     5941 ctgcagttca gaccaggcta cgatggcaag accagcatct ccagatggat cgtggaaggc
     6001 caagtgggcg ccattggaga tgaggaagag tgggtcaccc tgtacgagga agagaacgag
     6061 cctgacgcac agatgctgga aatccccaat ctgacccctt acacacacta ccggttccgg
     6121 atgaagcaag tgaacatcgt gggcccatct ccatacagcc ccagcagcag agtgattcag
     6181 actctgcagg cacctccaga tgtggcccca acatctgtga ccgtgcggac agcttccgag
     6241 acatccctga gactgagatg ggtgccactg ccagacagcc agtacaacgg caatcctgag
     6301 agcgtgggct acagaatcaa gtactggcgg agtgacctgc agtctagtgc cgtggctcag
     6361 gtggtgtctg accggctgga aagagagttc accatcgagg aactggaaga atggatggaa
     6421 tacgagctgc agatgcaggc cttcaatgcc gttggagctg gaccttggag cgaggttgtg
     6481 cggggcagaa caagagaaag cgtgccatct gccgctccag agaatgtttc tgctgaggcc
     6541 gtgtctagca cccagatcct gctgacatgg acctccgtgc cagagcagga ccagaatgga
     6601 ctgatcctgg gctacaagat cctgttcaga gccaaggatc tcgaccccga gcctagatct
     6661 cacatcgtgc ggggaaatca cacccagtct gctctgctgg ccggactgag aaagttcgtg
     6721 ctgtatgagc tgcaggtcct ggcctttacc agaatcggca atggggtgcc aagcacaccc
     6781 ctgattctgg aaagaacaaa ggacgacgcc cctggacctc ctgtcagact ggtttttcct
     6841 gaagtgcggc tgacctctgt gcgcattgtg tggcaaccac cagaggaacc caacggaatc
     6901 atcctgggat atcagatcgc ctaccggctg gcctctagca gccctcacac attcacaaca
     6961 gtggaagtgg gagccaccgt gcggcagttt acagctacag atctggcccc tgagtccgcc
     7021 tacatcttca gactgagcgc caagactaga caaggctggg gcgaacctct ggaagccaca
     7081 gtgatcacca ccgagaagag agaaaggccc gctccaccta gagagctgct ggttcctcag
     7141 gctgaagtga ccgccagatc tctgagactc cagtgggttc caggctctga tggcgcctct
     7201 cctatccggt actttaccat gcaagtgcgc gagctgccaa gaggcgagtg gcagacatac
     7261 agcagcagca tctctcacga ggccacagcc tgtgtggtgg acagactgag gcctttcacc
     7321 agctacaagc tgcggctgaa ggccaccaac gatatcggcg atagcgactt cagcagcgaa
     7381 accgaggctg tgactaccct gcaggatgtt cctggcgaac cacctggctc tgtgtccgcc
     7441 acacctcaca caacaagctc cgttctgatc caatggcagc cacctaggga cgagtctctg
     7501 aacggcctgc tccagggata cagaatctac taccgcgagc tggaatatga ggccggcagc
     7561 ggaacagagg ctaagaccct gaagaaccct atcgctctgc acgccgaact gacagcccag
     7621 tccagcttta agaccgtgaa tagcagctcc accagcacca tgtgcgaact gacccatctg
     7681 aagaaatatc ggcgctacga agtgatcatg acggcctaca acatcatcgg cgagagccca
     7741 gcctctgctc ctgtggaagt gtttgtggga gaagctgccc ctgccatggc tcctcagaat
     7801 gtccaagtga cacctctgac tgccagccag ctcgaagtta catgggaccc acctccacca
     7861 gaaagccaga acggaaacat tcaggggtac aagatctact attgggaagc cgactctcag
     7921 aacgagacag agaagatgaa ggtgctgttt ctgcccgagc ctgtcgtgcg cctgaagaat
     7981 ctgaccagcc acacaaagta cctggtgtct atcagcgcct tcaacgctgc aggcgacgga
     8041 cctaagtctg atcctcagca gggaagaaca caccaagcag ccccaggcgc tccaagcttt
     8101 ctggcctttt ctgagatcac cagcactacc ctcaacgtgt cctggggaga accagccgcc
     8161 gctaatggca ttctgcaggg atatagagtg gtgtacgagc ctctggctcc agtgcaaggc
     8221 gtgtccaaag tggtcaccgt ggaagtccgc ggcaattggc agagatggct gaaagtgcgg
     8281 gatctgacca agggcgtgac ctacttcttt cgggtgcaag ccaggaccat cacatacgga
     8341 ccagaactgc aggccaacat cacagccgga ccagctgagg gttctcctgg aagccctagg
     8401 gatgtgctgg ttacaaagag cgcctctgag ctgaccctgc aatggacaga aggacactcc
     8461 ggcgataccc ctaccacagg ctatgtgatt gaggccagac ctagcgacga aggactgtgg
     8521 gacatgttcg tgaaggacat cccacgcagc gccacctctt acaccctgag cctggataag
     8581 ctgagacagg gcgtcacata cgaattcaga gtggtggctg tgaacgaggc cggctacggc
     8641 gaaccttcta atcctggcgc gccgggcggc gacaagaccc acacctgccc cccctgccca
     8701 gctccagaac tgctgggagg gccttccgtg ttcctgttcc cccccaagcc caaggacacc
     8761 ctgatgatca gcaggacccc cgaggtgacc tgcgtggtgg tggccgtgtc ccacgaggac
     8821 ccagaggtga agttcaactg gtacgtggac ggcgtggagg tgcacaacgc caagaccaag
     8881 cccagagagg agcagtacaa cagcacctac agggtggtgt ccgtgctgac cgtgctgcac
     8941 caggactggc tgaacggcaa agaatacaag tgcaaagtct ccaacaaggc cctggctgcc
     9001 ccaatcgaaa agacaatcag caaggccaag ggccagccac gggagcccca ggtgtacacc
     9061 ctgcccccca gccgggagga gatgaccaag aaccaggtgt ccctgacctg tctggtgaag
     9121 ggcttctacc ccagcgatat cgccgtggag tgggagagca acggccagcc cgagaacaac
     9181 tacaagacca cccccccagt gctggacagc gacggcagct tcttcctgta cagcaagctg
     9241 accgtggaca agtccaggtg gcagcagggc aacgtgttca gctgcagcgt gatgcacgag
     9301 gccctgcaca accactacac ccagaagtcc ctgagcctga gccccggcgg cagcggtctt
     9361 aatgatattt tcgaggctca gaagattgag tggcatgaac accaccacca tcaccactaa
     9421 ggccggcctg tagtcactgg cacaacgcgt tacccggtaa gccaatcggg tatacacggt
     9481 cgtcatactg cagacagggt tcttctactt tgcaagatag tcttgagtag taaaataaat
     9541 agatagagaa aatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9601 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9661 gaggaaattg catcacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     9721 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9781 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     9841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     9901 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     9961 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10021 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
    10081 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
    10141 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
    10201 atcggcatag tataatacga caaggtgagg aactaaacca tgaccgagta caagcccacg
    10261 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
    10321 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
    10381 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
    10441 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
    10501 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
    10561 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
    10621 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
    10681 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacatccgc gccccgcaac
    10741 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
    10801 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac acgtgctacg agatttcgat
    10861 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    10921 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    10981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    11101 ataccgtcga cctctagcta gagcttggcg taatcatggt cattaccaat gcttaatcag
    11161 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    11221 cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg cgatgatacc
    11281 gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag ccggaagggc
    11341 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    11401 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccatcgctac
    11461 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11521 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    11581 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    11641 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11701 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11761 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11821 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11881 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11941 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    12001 catattcttc ctt
//
