LOCUS       sequence-383741-        9094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses inhibitory opsin gtACR2 and GFP in turning associated 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Kumar S, Sharma AK, Tran A, Liu M, Leifer AM
  TITLE     Inhibitory feedback from the motor circuit gates mechanosensory 
            processing in Caenorhabditis elegans.
  JOURNAL   PLoS Biol. 2023 Sep 21;21(9):e3002280. doi: 
            10.1371/journal.pbio.3002280. eCollection 2023 Sep.
  PUBMED    37733772
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-09-21"; volume: "21(9):e3002280. doi"; pages: " 
            10.1371/journal.pbio.3002280. eCollection 2023 Sep"
COMMENT     Sequence Label: pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR
FEATURES             Location/Qualifiers
     source          1..9094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     397..416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(635..739)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(765..1220)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(902..923)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1114..1133
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1347..1369
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1361..1378
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1362..1378
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1388..1407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1388..1406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5934..5962)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6710..6730
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7668..7688)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7668..7686)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7707..7723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7707..7723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7720..7742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7731..7747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7755..7761,7762..7779,7780..7785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7800..7821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7938..7955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8109..8697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8189..8208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8868..9094,1..565,566..634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
       61 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
      481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
      541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
      601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
      721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
      781 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
      841 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
      901 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
      961 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     1021 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     1081 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     1141 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     1201 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     1261 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     1321 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     1381 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggac tagtggatcc
     1441 cccgggctgc agaagctttg atttagaaat tgtagtttcc taagcctaaa tgtacatatt
     1501 acttttgggt tatgcctgag aattaccaat gattgttttg ttcaataaga attatcattt
     1561 acagagaaac aagatccatt gcttttatcg attcgagttg tgacttttta ggataaaatt
     1621 tccttgaaaa acgttacagc gacagacatc attttcgacg ttgaatctga aatcgtctgt
     1681 aactcaagaa aaaatcgctt ccacgaaaat aaatttatat aaagttattt tcataaaatt
     1741 aactgggact tttgggtgat tttgggagtt ttcggcaaac catcaaatcg ctaattgaca
     1801 aaataacaaa aaaggtattt aaacgatttt tagataaaat tcttaaattt taaaacagtg
     1861 aaactaaaac gggctggcat ttatccttgt agcgactcaa cttaatatta gaacttgact
     1921 tcacaattta acagacacga cgtctgagga actttttcgg ttgtttgcat ttttgatagc
     1981 tcactgtttt atgcccatat acgttttatt tcttatttat ctcaaaccgt cttccctcaa
     2041 acttgaataa atcttcacaa gactttttgg gagtttctgg cggaccatca aatcgctaat
     2101 tgaattcgcg cattttcgtg ttttgagtgc gtccgcatcc ttgcgagggg aaatacgatt
     2161 tacatcgatc tcgagataat tcgattcaag ctgttgcaca cgtcctcccg acaaacactg
     2221 gagaagagca cccaccagcc accgacggtc gacgactttt gacttcaaat tggatgtcaa
     2281 caactttcta ttaagcatgt tttgaaatgg gatacaaata aaagtaaaag aaagagattc
     2341 aaacatggat attgaaaaaa agtaattcag atatacacta taaagagggc agactattag
     2401 tatggttttt ttcaaatgaa ggagtgacac ggagtatcta aaaggttaac aaagttaaag
     2461 gttaaaaata acatttgaaa aaagatttaa tctagaatta tacgaatttt caggtaatgc
     2521 ttttttgatg tgaaaatgca atttgaactc tgaaaagttg gtaaatcaag gttattttat
     2581 tttagatcaa aactgagcgg cttgttcgtc ggctggtaga tcaaatgtga actttttaat
     2641 gtttcagcac gtactgattt ggaaattgtt acttcagttg aataaaacta gtaaatcaaa
     2701 aaaaaatcta aaatttacca tgtgttggcg gaattgattt attgtgatgt gtattggatt
     2761 ttttgtgtat tattgtttct tatgacacga ttcgtttgga atcgtattaa ataaataaaa
     2821 aataaaaaaa aaattgtcca cacgttcaag aaatacgtaa ttttaaccta ccagccgaca
     2881 gccgacaacc gacgtctgaa cactataaaa atatctaaat cgtggctggt tttttttgaa
     2941 gttttttttc taagaagtat ttcaagatca accaatcaaa attttataat tattctcaaa
     3001 ttgtcatttt ctgatactgt gaaaagtaaa caatttttta ccagtggttt agatttgctg
     3061 catgtttttt ttttcatttc cagtcagttt ctggagcaaa ataacgtcct atatctcttc
     3121 aggaagaggt ttttcagatt tcaattccaa tcttcaaccc tcccacaatc ctaacaacta
     3181 atagtattaa ttttggtaga ataataagat gcttccaatt tgatgcacac gtgtcgagtg
     3241 gaatgcgcca aatggtggtg tgtaggtgtg cttcttcccc gtctgttgtt ctttttgaga
     3301 gcccccagag ccaattcaca ttaatccgat aactcttgtt atatatgtat gtgtatcgaa
     3361 tccgaaagga tcactatttt aacaatagtc cttcggtatc actacctcat tcatgttttc
     3421 ctcagcggca tcctatgaaa acggcaggcc tggcagattg tgcgacatga attgatgctc
     3481 attagcatga gacatccaag tcctcaagac tggtggcagt aacaagcaaa ctagtcagga
     3541 gcctccaatt ttggtggctc tattatctgg ccgaacaatt tacccttatt gaaacagata
     3601 ttagtcacct tttcctattt tttccgaaaa acttggaaac gtaatatggg atacgcagta
     3661 gccatggttt cattatttga gagaggggtt gcaaaatatc tctacttact tattatcttc
     3721 tttatgataa tattttgcct ccaaagccat tatcacacat attttattcg caacggacac
     3781 ctaggatcat ttttgaaact tgtgtagtat aaccccacat tttgaataca tagttcgaat
     3841 ttattgtata gaaaaataca aatttctaag gctgaaacgc aatatgtgta tcattttaat
     3901 taaattggat gaaattctag agttctaaca taatttttaa taaacaggat tataatattc
     3961 aagtcccata ttgccttgca cccaattgtt taattttgtc agcttttatc tcatttttag
     4021 tttttttttg cattttcact gtattctaga ggtggacagc aattctacaa aattacctat
     4081 gttccatttt gttccaactt tttctattga aaattgatat taaattaagg gaaattactt
     4141 aattagtttg aaaatgacat tcattgaaac gttcaagaaa atgctgaaaa ttggttgatt
     4201 ttcaagtatc gaatagtcga aatatttaat ttttcaggac agtttagagc acttttgaaa
     4261 acgtttcagc taaaataaat agattttttc ggaaaaaaaa accatctcaa aatatgcatt
     4321 tttgttcaat ggcctggatg ccagaccatt tcttaaaatg tttaatgctc taataaaaac
     4381 tatcaaacta attttttttt tgaaatttag ctgtttattt attcatactt catatgatat
     4441 taattattct cgatattaac actttatttg cacttgataa gcattaaata atttcctatt
     4501 gttataactt taatttggga actttcagat tccggagatc tgggctcctt cttttgccgg
     4561 acactcctac gcaagcagcc aaccacatcc gccttgccgc ccaatagtga gcatcccagt
     4621 gggcggggcg agcttctagt gaggcctttt cgacagaaca acagactaga aggaagactt
     4681 ttgcgtcggt gctcgtcgac tcgttagtgg tccttgttgt cttgttatct acagctcggc
     4741 cggcagttcg ttaagaaagc agctgtcaca agttgcacca tggcatcaca ggtcgtctat
     4801 ggggaatggg catcaactca caccgagtgc tacaatatgt ccagaatcga ctccaccttc
     4861 gtctcactgc tgcagctggt gtgggccgtg gtctcaggct gccagacaat cttcatgatt
     4921 agcagggctc ccaaggtccc ttgggagtcc gtgtacctgc cctttgtcga atctatcact
     4981 tatgcactgg ccagtactgg caacggaacc ctgcagatga gggacggcag attctttcct
     5041 tggagccgaa tggcctcctg gctgtgcacc tgtccaatca tgctgggaca gatttctaac
     5101 atggctctgg tgaagtacaa aagtatccca ctgaatccca ttgcacaggc cgcttccatc
     5161 attagagtgg tcatgggaat caccgctaca atttctcccg cagagtatat gaagtggctg
     5221 ttctttttct ttggggccac ctgcctggtg ttcgagtaca gtgtggtctt cacaatcttt
     5281 caagtggggc tgtatggctt tgaatctgtc gggacacctc tggcacagaa ggtggtggtg
     5341 aggatcaaga tgctgcgcct gatcttcttt attgcttgga ctatgttccc aatcgtgtgg
     5401 ctgatttcac ccaccggagt ctgcgtgatc cacgagaacg tgagcgccat tctgtacctg
     5461 ctggctgacg gcctgtgcaa aaatacatat ggagtgatcc tgtggagcac tgcctgggga
     5521 gtgctggaag ggaagtggga ccccgcatgt ctgccagggc aggagaaacc cgaagccgac
     5581 gatccttttg gcctgaatca cgagaagaat gcccctccta acgacgaagt caatatcaga
     5641 atgtttggaa ggggtaccgc tgtctcatcc tactttcacc tagttaactg cttgtcttaa
     5701 aatctatgct tctctttagt atctaaaatt ttcctagaag cttacaagta tataaatggt
     5761 ctcttctcaa taaaggttgt atatttattc atcttattga atctgccatt tcctcgtttt
     5821 tgcgagttta tataccttcc aattttcttt ctattgtatt ttcaacttct aattttaatt
     5881 cagggaaact gcttcaacgc atcatgagta aaggagaaga acttttcact ggagttgtcc
     5941 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     6001 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     6061 tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
     6121 ttcagccaac acttgtcact actttctgtt atggtgttca atgcttctcg agatacccag
     6181 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     6241 gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa cagttcggta
     6301 ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa ggtgataccc
     6361 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     6421 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
     6481 atggaatcaa agttgtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     6541 ttcagaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     6601 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     6661 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     6721 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taaggcgccg
     6781 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     6841 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     6901 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     6961 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     7021 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     7081 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     7141 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     7201 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     7261 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     7321 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     7381 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     7441 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     7501 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     7561 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     7621 actagtcggc cgtacgggcc cttgggcccg gtacccagct tttgttccct ttagtgaggg
     7681 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7741 ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa
     7801 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8881 atcagtgagg cacctatctc agcgatctgy ctatttcgtt catccatagt tgcctgactc
     8941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9061 agggccgagc gcagaagtgg tcctgcaact ttat
//
