LOCUS       sequence-383748-        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-KIR3DL2-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        3105..3484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3485..3688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3639..3659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3685..3709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3919..3937
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3919..3936
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3950..3969
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5053..5088
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5098..5121
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6781..6825
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6826..6852
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(6992..7009)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6998..7109
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7227..7247)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7232..7561
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7412..7547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7474..7493
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7609..7656
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7675..8274
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7675..7694)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8171..8191
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8404..8525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8441..8460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8495..8514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8578..9369,9370..9438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9201..9220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9439..9448,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 gctgatgaat cccctaatga ttttggtaaa aatcattaag ttaaggtgga tacacatctt
     1681 gtcatatgat caaatggttt cgcgaaaaat caataatcag acaacaagat gtgcgaactc
     1741 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
     1801 agcttaacgt tggcttgcca cgcattactt gactgtaaaa ctctcactct taccgaactt
     1861 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
     1921 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt
     1981 atctgttcgg gcaatacgat gcccattgta cttgttgact ggtctgatat tcgtgagcaa
     2041 aaacgactta tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat
     2101 gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc
     2161 gaccttgcga gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt
     2221 aaagtgccat ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga
     2281 ggaaaagtac aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat
     2341 gatatgtcat ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca
     2401 atctcatgcc aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg
     2461 acacggactc attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca
     2521 tgggttctag caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc
     2581 tattcgaagc gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga
     2641 ctaggcctac gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc
     2701 gccctgatgc ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg
     2761 gacaagcact tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta
     2821 ggcatggaag ttttgcggca ttctggctac acaataacaa gggaagactt actcgtggct
     2881 gcaaccctac tagctcaaaa tttattcaca catggttacg ctttggggaa attatgaggg
     2941 gatctctcag tgcacagtgg cgttgactca atattggcca ttagccatat tattcattgg
     3001 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
     3061 tatgtacatt tatattggct catgtccaat atgaccgcca tgttggcatt gattattgac
     3121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     3721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
     3781 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
     3841 agtctatagg cccaccccct tggcttcgtt agaacgcggc tacaattaat acataacctt
     3901 atgtatcata cacatacgat ttaggtgaca ctatagaata acatccactt tgcctttctc
     3961 tccacaggtg tccactccca ggtccaactg cacctcggtt ctatcgaaaa cgcgcctgct
     4021 agcgccacca tgaagaccat cattgccctg agctacatct tctgcctggt gttcgctgcg
     4081 gccgccttga tgggaggcca agataagcct tttctgagcg ccagacctag caccgtggtt
     4141 cctagaggtg gacatgtggc cctgcagtgt cactacagac ggggcttcaa caacttcatg
     4201 ctgtacaaag aggaccgctc tcacgtcccc atcttccacg gcagaatctt ccaagagtcc
     4261 ttcatcatgg gccccgtgac accagctcac gccggcacat atagatgcag aggcagcaga
     4321 cctcacagcc tgacaggatg gtccgctcct agcaaccctc tggtcatcat ggtcaccggc
     4381 aaccacagaa agcctagcct gcttgctcat cccggacctc tgctgaagtc tggcgagaca
     4441 gtgattctgc agtgttggag cgacgtgatg ttcgagcact tctttctgca cagagagggc
     4501 atcagcgagg accctagtag actcgtggga cagattcacg atggcgtgtc caaggccaat
     4561 ttcagcatcg gccctctgat gcctgtgctg gccggcacct acagatgtta tggctctgtg
     4621 ccacacagcc cctaccagct gtctgctcct agcgatcccc tggacatcgt gattaccggc
     4681 ctgtacgaga agccaagcct gtctgcacag cctggaccta cagtgcaggc cggcgaaaat
     4741 gtgaccctga gctgtagcag ctggtccagc tacgacatct accacctgag cagagaaggc
     4801 gaggcccacg agagaaggct gagagccgtg cctaaagtga accggacctt ccaggccgat
     4861 tttcctctgg gacctgccac acacggcggc acctatcggt gttttggcag ctttagagcc
     4921 ctgccttgcg tgtggtccaa cagctctgat ccactgctgg tgtccgtgac cggcaatcct
     4981 tctagctcct ggccttctcc aaccgagcct agcagcaaga gcggcatctg cagacatctg
     5041 catggcgcgc cggaagatca agttgatcca agattgattg atggcaaggg cggatctctg
     5101 gaggtgctgt tccagggacc aggcggaagc ggcaatcccg gcggcgatct ggccccccag
     5161 atgctgcgcg agctgcagga gaccaatgcc gccctgcagg acgtgcgcga gctgctgcgc
     5221 caccgcgtga aggagatcac cttcctgaag aacaccgtga tggagtgcga cgcctgcggt
     5281 atgcagccct ctagaggctc cggctccatc atcccagttg aggaggagaa cccggacttc
     5341 tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc tgcacagaca
     5401 gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac ggtgacagct
     5461 gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc cctggccatg
     5521 gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca tgtgccagac
     5581 agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca gaccattggc
     5641 ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga ggtcatctcc
     5701 gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac cacacgagtg
     5761 cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg gtactcggac
     5821 gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac gcagctcatc
     5881 tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt tcgcatggga
     5941 accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct ggacgggaag
     6001 aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact
     6061 gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct ctttgagcct
     6121 ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct gatggagatg
     6181 acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct cttcgtggag
     6241 ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact gactgagacg
     6301 atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga ggacacgctg
     6361 agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg
     6421 agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta cacggtcctc
     6481 ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga tgttaccgag
     6541 agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga cgaagagacc
     6601 cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc
     6661 gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct ggagccctac
     6721 accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc gggtggcgga
     6781 ggactgaacg acatcttcga ggctcagaaa atcgaatggc acgaacatca ccatcaccat
     6841 caccatcacc attaaggccg gcctgtagtc actggcacaa cgcgttaccc ggtaagccaa
     6901 tcgggtatac acggtcgtca tactgcagac agggttcttc tactttgcaa gatagtcttg
     6961 agtagtaaaa taaatagata gagaaaatca gcctcgactg tgccttctag ttgccagcca
     7021 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     7081 ctttcctaat aaaatgagga aattgcatca caacactcaa ccctatctcg gtctattctt
     7141 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7201 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7261 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7321 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7381 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7441 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7501 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7561 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     7621 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatgacc
     7681 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7741 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     7801 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7861 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7921 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7981 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8041 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8101 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagaca
     8161 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8221 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacacgtg
     8281 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8341 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8401 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8461 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8521 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatta
     8581 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8641 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8701 cgctgcgatg ataccgcgag aaccacgctc accggctccg gatttatcag caataaacca
     8761 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8821 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8881 tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8941 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9001 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9061 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9121 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9181 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9241 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9301 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9361 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9421 gaaatgttga atactcatat tcttcctt
//
