LOCUS       sequence-38375-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Nguyen AW, Daugherty PS
  TITLE     Evolutionary optimization of fluorescent proteins for intracellular 
            FRET.
  JOURNAL   Nat Biotechnol. 2005 Mar;23(3):355-60. doi: 10.1038/nbt1066
  PUBMED    15696158
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2005-03"; volume: "23"; issue: "3"; pages: 
            "355-60"
COMMENT     Sequence Label: pYPet-His
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(85..102)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(103..816)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYK"
ORIGIN
        1 ttctctcatc cgccaaaaca gccaagcttg catgcctgca ggtcgactct agaggatccc
       61 cgggtaccgg ccaccttggc cttagtggtg gtggtggtgg tgtttgtaca attcattcat
      121 accctcggta ataccagcag cagtcaaaaa ttctaacaag accatgtggt ctctcttttc
      181 gtttggatct ttgaataagg cagattgata ggataagtaa tggttgtctg gtaacaagac
      241 tggaccatca ccaattggag tattttgttg ataatggtca gctaattgaa caccaccatc
      301 ttcaatgttg tgtctaattt tgaagttagc tttgatacca ttcttttgtt tgtcagcagt
      361 gatgtaaaca ttgtgagagt tatagttgta ttccaatttg tgacctaaaa tgttaccatc
      421 ttctttaaaa tcaatacctt ttaattcgat tctattaact aaggtatcac cttcaaactt
      481 gacttcagct ctggtcttgt agttaccgtc atctttgaaa aaaatagttc tttcttgaac
      541 ataaccttct ggcatggcag acttgaaaaa gtcatgttgt ttcatatgat ctgggtatct
      601 agcaaaacat tgaacaccat aacctaaagt agtgactaag gttggccatg gaactggcaa
      661 tttaccagta gtacagagta attttaaggt caatttaccg tacgtagcat caccttcacc
      721 ttcaccggag acagaaaatt tgtgaccatt aacatcacca tctaattcaa ccaaaattgg
      781 gacaacacca gtgaataatt cttcaccttt agacatcgtt tcactcctgg cct
//
