LOCUS       sequence-383792-        1007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNeonGreen expression with TRE3g promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Qian Y, Li J, Zhao S, Matthews EA, Adoff M, Zhong W, An X, Yeo M, 
            Park C, Yang X, Wang BS, Southwell DG, Huang ZJ
  TITLE     Programmable RNA sensing for cell monitoring and manipulation.
  JOURNAL   Nature. 2022 Oct;610(7933):713-721. doi: 10.1038/s41586-022-05280-1.
            Epub 2022 Oct 5.
  PUBMED    36198803
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05280-1"; journalName: "Nature"; date: 
            "2022-10"; volume: "610"; issue: "7933"; pages: "713-721"
COMMENT     Sequence Label: pAAV-TRE3g-mNeon-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    230..251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(266..271,272..289,290..296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    304..320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     309..331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     328..344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     328..344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   382..511
                     /label=AAV2 ITR
     promoter        581..954
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    589..607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    625..643
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    661..679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    697..715
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    733..751
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    769..787
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    805..823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     867..891
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      961..970
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
       61 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      121 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccagat
      361 ttaattaagg ccttaattag gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
      421 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
      481 ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac
      541 ttatctacgt agccatgctc taggaagatc ggaattcctc gagtttactc cctatcagtg
      601 atagagaacg tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac
      661 tccctatcag tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta
      721 tgaccagttt actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg
      781 atagagaacg tatatccagt ttactcccta tcagtgatag agaacgtata agctttaggc
      841 gtgtangngg gcgcctataa aagcagagct cgtttagtga accgtcagat cgcctgngca
      901 attccacana cttttgtctt ataccaactt tccgtaccac ttcnaccctc gtaagtcgac
      961 gccaccatgg tgagcanggn cgnggaggat nannatnnnt cnctncc
//
