LOCUS       sequence-383796-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Qian Y, Li J, Zhao S, Matthews EA, Adoff M, Zhong W, An X, Yeo M, 
            Park C, Yang X, Wang BS, Southwell DG, Huang ZJ
  TITLE     Programmable RNA sensing for cell monitoring and manipulation.
  JOURNAL   Nature. 2022 Oct;610(7933):713-721. doi: 10.1038/s41586-022-05280-1.
            Epub 2022 Oct 5.
  PUBMED    36198803
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-05280-1"; journalName: "Nature"; date: 
            "2022-10"; volume: "610"; issue: "7933"; pages: "713-721"
COMMENT     Sequence Label: CAG-BFP-sesRNA-GFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    383..504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(420..439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     474..493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             653..664
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
       61 tctccccgcg cgttggccga ttcattaatg cagctgcgcg ctcgctcgct cactnnnnaa
      121 gnaannaang cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
      181 gcgcanagag ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc
      241 gccatgctac ttatctacgt agccatgctc taggaagatc cttatcgatt ttaccacatt
      301 tgtagaggtt nnacttgctt taaaaaacct cccacatctc cccctgaacc tgaaacataa
      361 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatgntt acaaataaag
      421 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      481 gtccaaactc atcaatgtat cttatcatgt ctgctcgaag cggccgcccg ggtcgacggt
      541 atcgatgcgg ggaggcggcc caaagggaga tccgactcgt ctgaggncga aggcgaagac
      601 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
      661 ccgaagggac gtagcanaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
      721 gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
      781 gagcccctgt ccagcagcgg gcaaggnngg nggngatgag ttccgccgtg nataggaggg
      841 gnaagcgaaa ntcccgnaaa gagctgacnn tggngcatgc cccacagtgg ggtnngtcan
      901 caanncnnng cnnnccacgc cnngtgctgn naacgggcnn aanncncntn annnnnnncn
      961 cnng
//
