LOCUS       sequence-383799-        7671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express enAsCas12a-mSA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Liang SQ, Liu P, Smith JL, Mintzer E, Maitland S, Dong X, Yang Q, 
            Lee J, Haynes CM, Zhu LJ, Watts JK, Sontheimer EJ, Wolfe SA, Xue W
  TITLE     Genome-wide detection of CRISPR editing in vivo using GUIDE-tag.
  JOURNAL   Nat Commun. 2022 Jan 21;13(1):437. doi: 10.1038/s41467-022-28135-9.
  PUBMED    35064134
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-01-21"; volume: "13(1):437. doi"; pages: " 
            10.1038/s41467-022-28135-9"
COMMENT     Sequence Label: enAsCas12a-mSA
FEATURES             Location/Qualifiers
     source          1..7671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          813..945
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     975..994
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        975..993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1014..1023
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1029..1055
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1092..1118
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1164..5081
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4801..4810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5094..5114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5115..5204
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             5217..5243
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             5286..5627
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     primer_bind     complement(5656..5672)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5656..5672)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5685..5711
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5754..5780
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     primer_bind     complement(5844..5863)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    5873..5954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5898..5917
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6152..6740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6232..6251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6821..7615)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6821..7615)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6932..6951)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7542..7561
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacaggggta ccgaagccgc
      961 tagcgctacc ggtctaatac gactcactat agggcgaatt gggtaccggg cccgccacca
     1021 tggctagccc cgccgccaag cgcgtgaagc tggacggcgg cagcggcggc ggcagcggcg
     1081 gcggcagcgg cccggcagct aagagggtga aactggatgg cggtagcggc ggaggcagcg
     1141 gtggcggcag cggccccctc gagacacagt tcgagggctt taccaacctg tatcaggtga
     1201 gcaagacact gcggtttgag ctgatcccac agggcaagac cctgaagcac atccaggagc
     1261 agggcttcat cgaggaggac aaggcccgca atgatcacta caaggagctg aagcccatca
     1321 tcgatcggat ctacaagacc tatgccgacc agtgcctgca gctggtgcag ctggattggg
     1381 agaacctgag cgccgccatc gactcctata gaaaggagaa aaccgaggag acaaggaacg
     1441 ccctgatcga ggagcaggcc acatatcgca atgccatcca cgactacttc atcggccgga
     1501 cagacaacct gaccgatgcc atcaataaga gacacgccga gatctacaag ggcctgttca
     1561 aggccgagct gtttaatggc aaggtgctga agcagctggg caccgtgacc acaaccgagc
     1621 acgagaacgc cctgctgcgg agcttcgaca agtttacaac ctacttctcc ggcttttata
     1681 gaaacaggaa gaacgtgttc agcgccgagg atatcagcac agccatccca caccgcatcg
     1741 tgcaggacaa cttccccaag tttaaggaga attgtcacat cttcacacgc ctgatcaccg
     1801 ccgtgcccag cctgcgggag cactttgaga acgtgaagaa ggccatcggc atcttcgtga
     1861 gcacctccat cgaggaggtg ttttccttcc ctttttataa ccagctgctg acacagaccc
     1921 agatcgacct gtataaccag ctgctgggag gaatctctcg ggaggcaggc accgagaaga
     1981 tcaagggcct gaacgaggtg ctgaatctgg ccatccagaa gaatgatgag acagcccaca
     2041 tcatcgcctc cctgccacac agattcatcc ccctgtttaa gcagatcctg tccgatagga
     2101 acaccctgtc tttcatcctg gaggagttta agagcgacga ggaagtgatc cagtccttct
     2161 gcaagtacaa gacactgctg agaaacgaga acgtgctgga gacagccgag gccctgttta
     2221 acgagctgaa cagcatcgac ctgacacaca tcttcatcag ccacaagaag ctggagacaa
     2281 tcagcagcgc cctgtgcgac cactgggata cactgaggaa tgccctgtat gagcggagaa
     2341 tctccgagct gacaggcaag atcaccaagt ctgccaagga gaaggtgcag cgcagcctga
     2401 agcacgagga tatcaacctg caggagatca tctctgccgc aggcaaggag ctgagcgagg
     2461 ccttcaagca gaaaaccagc gagatcctgt cccacgcaca cgccgccctg gatcagccac
     2521 tgcctacaac cctgaagaag caggaggaga aggagatcct gaagtctcag ctggacagcc
     2581 tgctgggcct gtaccacctg ctggactggt ttgccgtgga tgagtccaac gaggtggacc
     2641 ccgagttctc tgcccggctg accggcatca agctggagat ggagccttct ctgagcttct
     2701 acaacaaggc cagaaattat gccaccaaga agccctactc cgtggagaag ttcaagctga
     2761 actttcagat gcctacactg gccagaggct gggacgtgaa tagagagaag aacaatggcg
     2821 ccatcctgtt tgtgaagaac ggcctgtact atctgggcat catgccaaag cagaagggca
     2881 ggtataaggc cctgagcttc gagcccacag agaaaaccag cgagggcttt gataagatgt
     2941 actatgacta cttccctgat gccgccaaga tgatcccaaa gtgcagcacc cagctgaagg
     3001 ccgtgacagc ccactttcag acccacacaa cccccatcct gctgtccaac aatttcatcg
     3061 agcctctgga gatcacaaag gagatctacg acctgaacaa tcctgagaag gagccaaaga
     3121 agtttcagac agcctacgcc aagaaaaccg gcgaccagaa gggctacaga gaggccctgt
     3181 gcaagtggat cgacttcaca agggattttc tgtccaagta taccaagaca acctctatcg
     3241 atctgtctag cctgcggcca tcctctcagt ataaggacct gggcgagtac tatgccgagc
     3301 tgaatcccct gctgtaccac atcagcttcc agagaatcgc cgagaaggag atcatggatg
     3361 ccgtggagac aggcaagctg tacctgttcc agatctataa caaggacttt gccaagggcc
     3421 accacggcaa gcctaatctg cacacactgt attggaccgg cctgttttct ccagagaacc
     3481 tggccaagac aagcatcaag ctgaatggcc aggccgagct gttctaccgc cctaagtcca
     3541 ggatgaagag gatggcacac cggctgggag agaagatgct gaacaagaag ctgaaggatc
     3601 agaaaacccc aatccccgac accctgtacc aggagctgta cgactatgtg aatcacagac
     3661 tgtcccacga cctgtctgat gaggccaggg ccctgctgcc caacgtgatc accaaggagg
     3721 tgtctcacga gatcatcaag gataggcgct ttaccagcga caagttcttt ttccacgtgc
     3781 ctatcacact gaactatcag gccgccaatt ccccatctaa gttcaaccag agggtgaatg
     3841 cctacctgaa ggagcacccc gagacaccta tcatcggcat cgatcggggc gagagaaacc
     3901 tgatctatat cacagtgatc gactccaccg gcaagatcct ggagcagcgg agcctgaaca
     3961 ccatccagca gtttgattac cagaagaagc tggacaacag ggagaaggag agggtggcag
     4021 caaggcaggc ctggtctgtg gtgggcacaa tcaaggatct gaagcagggc tatctgagcc
     4081 aggtcatcca cgagatcgtg gacctgatga tccactacca ggccgtggtg gtgctggaga
     4141 acctgaattt cggctttaag agcaagagga ccggcatcgc cgagaaggcc gtgtaccagc
     4201 agttcgagaa gatgctgatc gataagctga attgcctggt gctgaaggac tatccagcag
     4261 agaaagtggg aggcgtgctg aacccatacc agctgacaga ccagttcacc tcctttgcca
     4321 agatgggcac ccagtctggc ttcctgtttt acgtgcctgc cccatataca tctaagatcg
     4381 atcccctgac cggcttcgtg gaccccttcg tgtggaaaac catcaagaat cacgagagcc
     4441 gcaagcactt cctggagggc ttcgactttc tgcactacga cgtgaaaacc ggcgacttca
     4501 tcctgcactt taagatgaac agaaatctgt ccttccagag gggcctgccc ggctttatgc
     4561 ctgcatggga tatcgtgttc gagaagaacg agacacagtt tgacgccaag ggcacccctt
     4621 tcatcgccgg caagagaatc gtgccagtga tcgagaatca cagattcacc ggcagatacc
     4681 gggacctgta tcctgccaac gagctgatcg ccctgctgga ggagaagggc atcgtgttca
     4741 gggatggctc caacatcctg ccaaagctgc tggagaatga cgattctcac gccatcgaca
     4801 ccatggtggc cctgatccgc agcgtgctgc agatgcggaa ctccaatgcc gccacaggcg
     4861 aggactatat caacagcccc gtgcgcgatc tgaatggcgt gtgcttcgac tcccggtttc
     4921 agaacccaga gtggcccatg gacgccgatg ccaatggcgc ctaccacatc gccctgaagg
     4981 gccagctgct gctgaatcac ctgaaggaga gcaaggatct gaagctgcag aacggcatct
     5041 ccaatcagga ctggctggcc tacatccagg agctgcgcaa cggcaccggc gggcccaaga
     5101 agaagaggaa ggtataccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc
     5161 cggactatgc aggatcgtat ccttatgacg ttccagatta cgctggatcc gccgctccgg
     5221 cagctaagaa aaagaaactg gatttcgaat ccggagaatt cggggcccag ccggccagat
     5281 ctatggcgga agcgggtatc accggcacgt ggtacaacca gtctggttct accttcaccg
     5341 ttaccgcggg tgcggacggt aacctgaccg gtcagtacga aaaccgtgcg cagggcactg
     5401 gttgccagaa ctctccgtac accctgaccg gtcgttacaa cggtaccaaa ctggaatggc
     5461 gtgttgaatg gaacaactct accgaaaact gccactctcg taccgaatgg cgtggtcagt
     5521 accagggtgg tgcggaagcg cgtatcaaca cccagtggaa cctgacctac gaaggtggtt
     5581 ctggtccggc gaccgaacag ggtcaggaca ccttcaccaa agttaaaccg tctgcggcgt
     5641 ctggatcccc cgggggatcc actagttcta gaggatccgc cgctcccgcc gccaagcgcg
     5701 tgaagctgga cggcggcagc ggcggcggca gcggcggcgg cagcggctcc ggaccggcag
     5761 ctaagagggt gaaactggat tgataagcgg ccgccaccgc ggtggagctc cagcttttgt
     5821 tccctttagt gagggttaat ctcgatgagt ttggacaaac cacaactaga atgcagtgaa
     5881 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5941 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     6001 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtacttaa gagggggaga
     6061 ccaaagggcg agacgttaag gcctcacgtg acatgtgagc aaaaggccag caaaaggcca
     6121 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6181 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6241 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttacgg
     6301 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6361 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6421 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6481 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6541 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6601 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6661 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6721 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6781 ggaacgaaaa ctcacgttaa gggattttgg tcatgccgtc tcagaagaac tcgtcaagaa
     6841 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     6901 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     6961 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     7021 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     7081 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     7141 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctctc tcgatgcgat
     7201 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     7261 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     7321 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagtacag
     7381 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     7441 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     7501 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     7561 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcataatat
     7621 tattgaagca tttatcaggg ttcgtctcgt cccggtctcc tcccatgcat g
//
