LOCUS       sequence-383812-        9399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpress BCAT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9399)
  TITLE     Chen lab plasmids Dec 2022
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCDH-BCAT2
FEATURES             Location/Qualifiers
     source          1..9399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2293..2302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3475..3498
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        3541..3752
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3765..4033
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4062..4071
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4092..4754
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             4824..4877
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4878..5477
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4878..4897)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5374..5394
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5478..6066
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5531..5551)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5949..5960)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6140..6373
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6445..6566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6482..6501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6536..6555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6606..6741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6774..6790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6774..6790)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6787..6809)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6798..6814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6822..6828,6829..6846,6847..6852))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6867..6888
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7005..7022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7176..7764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7935..8726,8727..8795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8558..8577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8796..8900)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8968..8986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9024..9046)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9146..9165
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9359..9381
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9373..9390
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9374..9390
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagagcta gcgccaccat ggccgcagcc gctctggggc agatctgggc acgaaagctt
     2341 ctctctgtcc cttggcttct gtgtggtccc agaagatatg cctcctccag tttcaaggct
     2401 gcagacctgc agctggaaat gacacagaag cctcataaga agcctggccc cggcgagccc
     2461 ctggtgtttg ggaagacatt taccgaccac atgctgatgg tggaatggaa tgacaagggc
     2521 tggggccagc cccgaatcca gcccttccag aacctcacgc tgcacccagc ctcctccagc
     2581 ctccactact ccctgcagct gtttgagggc atgaaggcgt tcaaaggcaa agaccagcag
     2641 gtgcgcctct tccgcccctg gctcaacatg gaccggatgc tgcgctcagc catgcgcctg
     2701 tgcctgccga gtttcgacaa gctggagttg ctggagtgca tccgccggct catcgaagtg
     2761 gacaaggact gggtccccga tgccgccggc accagcctct atgtgcggcc tgtgctcatt
     2821 gggaacgagc cctcgctggg tgtcagccag cccacgcgcg cgctcctgtt cgtcattctc
     2881 tgcccagtgg gtgcctactt ccctggaggc tccgtgaccc cggtctccct cctggccgac
     2941 ccagccttca tccgggcctg ggtgggcggg gtcggcaact acaagttagg tgggaattat
     3001 gggcccaccg tgttagtgca acaggaggca ctcaagcggg gctgtgaaca ggtcctctgg
     3061 ctgtatgggc ccgaccacca gctcaccgag gtgggaacca tgaacatctt tgtctactgg
     3121 acccacgaag atggggtgct ggagctggtg acgcccccgc tgaatggtgt tatcctgcct
     3181 ggagtggtca gacagagtct actggacatg gctcagacct ggggtgagtt ccgggtggtg
     3241 gagcgcacga tcaccatgaa gcagttgctg cgggccctgg aggagggccg cgtgcgggaa
     3301 gtctttggct cgggcaccgc ttgccaggtc tgcccagtgc accgaatcct gtacaaagac
     3361 aggaacctcc acattcccac catggaaaat gggcctgagc tgatcctccg cttccagaag
     3421 gagctgaagg agatccagta cggaatcaga gcccacgagt ggatgttccc ggtggactac
     3481 aaagacgacg atgacaagtg aggccgcgaa ggatctgcga tcgctccggt gcccgtcagt
     3541 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     3601 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     3661 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     3721 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     3781 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     3841 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     3901 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     3961 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     4021 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg
     4081 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg
     4141 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga
     4201 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct
     4261 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca
     4321 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc
     4381 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg
     4441 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca
     4501 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct
     4561 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga
     4621 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg
     4681 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc
     4741 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg
     4801 gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg
     4861 aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     4921 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     4981 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     5041 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     5101 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     5161 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     5221 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     5281 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     5341 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     5401 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     5461 gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5521 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5581 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5641 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5701 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5761 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5821 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5881 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5941 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6001 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6061 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6121 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     6181 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6241 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6301 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6361 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6421 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6541 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6601 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6661 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6721 gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat catgtcatag
     6781 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6841 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6901 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6961 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7501 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7561 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7621 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7681 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7741 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7801 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7861 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7921 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7981 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8041 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8101 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8161 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8221 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8281 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8341 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8401 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8461 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8521 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8581 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8641 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8701 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8761 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8821 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8881 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8941 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     9001 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     9061 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     9121 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     9181 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     9241 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     9301 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     9361 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
