LOCUS       sequence-383828-        1385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GAL4 transcriptional activation domain (AD)-AP-1 u1A 
            fusion protein in yeast (yeast two-hybrid assays).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino 
            JS
  TITLE     The adaptor protein-1 mu1B subunit expands the repertoire of 
            basolateral sorting signal recognition in epithelial cells.
  JOURNAL   Dev Cell. 2013 Nov 11;27(3):353-66. doi: 
            10.1016/j.devcel.2013.10.006.
  PUBMED    24229647
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.10"; journalName: "Dev Cell"; date: 
            "2013-11-11- 11"; volume: "27"; issue: "3"; pages: "353-66"
COMMENT     Sequence Label: pACT2-u1A
FEATURES             Location/Qualifiers
     source          1..1385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..27
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7..33
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      231..240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tacccatacg atgttccaga ttacgctagc ttgggtggtc atatggccat ggaggccccg
       61 gggatccgaa ttcccgccat gtccgccagc gccgtctacg tactggatct gaagggcaag
      121 gtgctcatct gcaggaacta ccgtggggat gtggacatgt cagaggtgga gcacttcatg
      181 cccattctga tggagaagga ggaggagggc atgctgtcac ctatcttggc ccatggtggc
      241 gttcgtttca tgtggattaa gcacaacaac ctgtacctgg tcgccacttc aaaaaagaat
      301 gcttgtgtgt cactggtgtt ctccttcctc tacaaggtgg tacaggtctt ctccgagtac
      361 tttaaggagt tggaggagga gagcatccga gacaactttg tcatcatcta cgagctgcta
      421 gatgagctca tggactttgg ctacccgcag accactgaca gcaagatctt gcaggagtac
      481 atcactcagg aaggccacaa gctggaaacg ggggccccta ggcccccagc cacagtcacc
      541 aatgctgtgt cctggcgttc agaaggcatc aagtatcgga agaatgaagt attcctggat
      601 gtcattgagg ctgttaacct cttggtcagt gccaatggca acgtgctgcg cagtgagatt
      661 gtgggttcca tcaagatgcg ggtcttcctc tcaggcatgc ctgagttacg cctgggtctc
      721 aatgacaagg tcctcttcga caacacaggc cgagggaaga gcaagtcagt ggagctggag
      781 gatgtgaaat tccaccagtg tgtgcggctg tcacgttttg agaacgaccg cactatctcc
      841 ttcatcccac ccgacggaga gtttgaactc atgtcctacc gcctcaacac ccatgtgaag
      901 cctttgatct ggattgagtc cgtgattgag aagcattccc acagccgcat tgagtacatg
      961 gtcaaggcca agagccagtt caagaggcgg tcaacagcca acaatgtaga gatccatata
     1021 ccagtcccca acgatgctga ttcacccaag ttcaagacta cagtggggag tgtcaagtgg
     1081 gtccctgaaa acagtgagat cgtgtggtcc gtcaagtcct ttccgggtgg caaggagtac
     1141 ctgatgcggg cccactttgg ccttcccagt gtggaagctg aagacaagga gggaaagccc
     1201 cccatcagcg tcaagtttga gatcccctac ttcactacct ctggcatcca ggtgcgctac
     1261 ctgaaaatca ttgagaagag tgggtaccag gccctgccct gggtacgata tatcacacag
     1321 aacggagatt atcagctccg gacccagtga ctcgagagat ctatgaatcg tagatactga
     1381 aaaac
//
