LOCUS       sequence-383839-       11829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HA-tagged Creb5 in pInducer20 lentivirus destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11829)
  AUTHORS   Zhang CH, Gao Y, Jadhav U, Hung HH, Holton KM, Grodzinsky AJ, 
            Shivdasani RA, Lassar AB
  TITLE     Creb5 establishes the competence for Prg4 expression in articular 
            cartilage.
  JOURNAL   Commun Biol. 2021 Mar 12;4(1):332. doi: 10.1038/s42003-021-01857-0.
  PUBMED    33712729
REFERENCE   2  (bases 1 to 11829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-03-12"; volume: "4(1):332. doi"; pages: " 
            10.1038/s42003-021-01857-0"
COMMENT     Sequence Label: pInducer20-iCreb5(HA)
FEATURES             Location/Qualifiers
     source          1..11829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    92..554
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(148..165)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     375..394
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             570..1364
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             570..1364
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(624..643)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1234..1253
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1541..2129
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1594..1614)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2012..2023)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2204..2437
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2556..2574)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2642..2746
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2747..2815,2816..3607)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2965..2984)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3778..4366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4267..4286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4520..4537
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4777..4798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4813..4818,4819..4836,4837..4843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4851..4867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4856..4878
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4875..4891
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4875..4891
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4941..4960
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5152..5785
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5832..5957
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6454..6687
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6872..6916
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7209..7326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(7381..7399,7400..7422,7423..7441,7442..7464,7465..7483,
                     7484..7506,7507..7525,7526..7548,7549..7567,7568..7590,
                     7591..7609,7610..7632,7633..7651)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        7684..7722
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7719..7743
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7852..7876
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             7888..7908
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9448..9474
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9481..9507
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9508..9534
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(9554..9578)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9631..9657
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        9743..10954
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     10791..10810
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(11013..11630,11631..11636,11637..11672,11673..11675,
                     11676..11711,11712..11714,11715..11750,11751..11759)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
       61 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
      121 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
      181 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
      241 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
      301 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
      361 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
      421 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
      481 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
      541 aaacacgatg ataagcttac cggtccacca tgattgaaca agatggattg cacgcaggtt
      601 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
      661 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
      721 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
      781 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
      841 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
      901 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
      961 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     1021 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     1081 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     1141 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     1201 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     1261 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     1321 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgatgtaca agtaaagcgg
     1381 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1441 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag
     1501 tccctcccaa ttcgatatca agcttatcgc gatagatcct aatcaacctc tggattacaa
     1561 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     1621 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     1681 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     1741 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     1801 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     1861 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     1921 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     1981 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2041 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     2101 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     2161 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     2221 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     2281 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     2341 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     2401 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     2461 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     2521 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     2581 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     2641 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2701 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2761 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2821 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2881 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2941 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     3001 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3061 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3121 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3181 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3241 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3301 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3361 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3421 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3481 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3541 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3601 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3661 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3721 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3781 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3841 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3901 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3961 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4021 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4081 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4141 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4201 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4261 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4321 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4381 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4441 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4501 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4561 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
     4621 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4681 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
     4741 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4801 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4861 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
     4921 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4981 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     5041 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     5101 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     5161 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     5221 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     5281 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     5341 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     5401 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     5461 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     5521 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     5581 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     5641 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5701 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5761 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     5821 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     5881 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     5941 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     6001 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     6061 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     6121 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     6181 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     6241 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     6301 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     6361 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     6421 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     6481 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     6541 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     6601 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     6661 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     6721 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     6781 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     6841 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     6901 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     6961 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     7021 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     7081 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     7141 atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat ggcagtattc
     7201 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     7261 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     7321 aattttcggg tttattacag ggacagcaga gatccagttt ggactaggat cctttaccac
     7381 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     7441 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     7501 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     7561 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     7621 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt
     7681 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     7741 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     7801 agcctccgcg gccccgaact agtccagtgt ggtggaattc tgcagatatc aacaagtttg
     7861 tacaaaaaag caggctccgc ggccgccttg tttaacttta agaaggagcc cttcaccatg
     7921 atttatgagg aatccaagat gaatttggag caggagaggc cgtttgtctg cagtgcccca
     7981 ggctgctccc agcgcttccc aacagaggat catctgatga ttcataggca caagcatgaa
     8041 atgactttga agtttccctc aataaaaaca gacaatatgt tatcagatca aactccaacc
     8101 ccaacgagat tcctgaagaa ctgcgaggag gtggggctct tcagtgagtt ggactgctcc
     8161 ctagagcatg agttcaggaa ggctcaggag gaagagagca gcaagcggaa tatctcgatg
     8221 cataaccccg ttggtggggc catggcgggg cctggagctc accagctcgg cagcgcccgg
     8281 atgcccaacc atgacaccag cgttgtgatt cagcaagcca tgccgtcacc ccagtccagc
     8341 tctgtcatca cgcaggcccc ttccaccaac cgccagatcg ggcctgtccc aggctctcta
     8401 tcttctctgc tccatcttca caacagacag agacagccca tgccagcctc catgcctggg
     8461 accctgccca accctacaat gccgggctct tctgccgtct tgatgccaat ggagagacaa
     8521 atgtcagtga actccaacct cctgggaatg caaggtccaa atctcagcaa cccctgtgct
     8581 tctccccaag tccagccaat gcattcagaa gctaagatga ggctgaaggc tgcattgact
     8641 caccaccctg ctgccatgtc gaatgggaat atgaacacca tgggccacat gatggaaatg
     8701 atgggctccc ggcaggacca gacgccacac catcacatgc actcacaccc gcatcagcac
     8761 cagacactgc cggcccacca cccctacccg caccagcacc agcacccagc acaccaccct
     8821 catcctcaac cccatcacca gcagaaccac ccacatcatc actcccactc ccaccttcat
     8881 gcacacccag cacatcacca gacctcgcca cacccacccc tgcactccgg cacccaagca
     8941 caggtttcac cagcaacaca acagatgcag cccacccaga caatacagcc gccccagccc
     9001 acaggggggc gccggcgaag ggtggtggat gaggatcctg acgagaggcg gcggaaattt
     9061 ctggaacgga accgggcggc cgccacccgc tgcaggcaga agaggaaggt ctgggtgatg
     9121 tcactggaaa agaaagcaga agagctcacc cagacaaaca tgcagcttca gaacgaagtg
     9181 tccatgttga agaacgaggt ggcacagctg aagcagctgt tgttaacaca taaagactgc
     9241 cccataacag ccatgcagaa agaatcacaa ggatatctca gtccagagag tagccctcct
     9301 gcaagtccca cccccgcatg ctcacagcag caggtcatcc agcataacac catcaccact
     9361 tcctcggcag tcagcgaggt ggtaggaagc tctaccctca gccagctcac cactcacaga
     9421 acagacctga accccatcct cggaggttac ccttacgacg tcccagacta cgctggctcc
     9481 tacccttacg acgtcccaga ctacgcttac ccttacgacg tcccagacta cgcttaaaag
     9541 ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc
     9601 cgctcgagtc tagagggccc gcggttcgaa tacccatacg acgtcccaga ctacgcttag
     9661 taatgattaa ttaaactaga aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     9721 tggagtaacc cacccaagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
     9781 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct
     9841 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     9901 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     9961 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
    10021 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
    10081 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
    10141 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
    10201 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
    10261 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
    10321 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
    10381 gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
    10441 tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt
    10501 cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg
    10561 gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg
    10621 gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg
    10681 cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt
    10741 gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg
    10801 gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt
    10861 tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc
    10921 taaattctgg ccgtttttgg cttttttgtt agacgaagct tggtaccgag ctcggatctc
    10981 caccccgtac cggtcctgca gtcgaattca ccatgtctag actggacaag agcaaagtca
    11041 taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac
    11101 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
    11161 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
    11221 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
    11281 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
    11341 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
    11401 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
    11461 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
    11521 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
    11581 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
    11641 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
    11701 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac
    11761 taagtaagga tccgcggccg cactagagga atwcckcccs wctcscscss smycwmtccc
    11821 kcccccccc
//
