LOCUS       sequence-38384-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Tabor JJ, Levskaya A, Voigt CA
  TITLE     Multichromatic control of gene expression in Escherichia coli.
  JOURNAL   J Mol Biol. 2011 Jan 14. 405(2):315-24.
  PUBMED    21035461
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Jan 14. 405(2):315-24."
COMMENT     Sequence Label: pJT106b
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     160..182
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     RBS             205..216
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    323..344
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      347..390
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(394..412)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        477..581
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(800..819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcacgcttct ggtgccggaa accaggcaaa gcgccattcg ccattcaggc tgcgcaactg
       61 tctgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
      121 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      181 cgacgggatc tatcatctct agtatttctc ctctttctcc agtatatcac cgccagaggt
      241 aaaatagtca acacgcacgg tgttattcga ctataacaca ccattttctt gttgtcaacc
      301 tgtacgatcc tacaggtgct tactctagaa gcggccgcga attccagaaa tcatccttag
      361 cgaaagctaa ggattttttt tatctgaaat tctgcctcgt gatacgccta tttttatagg
      421 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg caccatggga aatgtgcgcg
      481 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      541 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      601 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      661 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      721 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      781 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
      841 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagta
//
