LOCUS       sequence-383847-        3984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_17_pKD46.10
FEATURES             Location/Qualifiers
     source          1..3984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        235..326
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(327..395,396..1187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1191..1230
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(1358..2308)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2356..2578
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2861..2947)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        3111..3145
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        3148..3197
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3263..3271
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
      241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      301 ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc
      361 cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga
      421 aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg
      481 gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag
      541 tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc
      601 gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa aaacatctga
      661 ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc gataacaccg
      721 cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg tttctgcata
      781 acatgggcga tcatgtgacc cgtctggatc gttgggaacc ggaactgaac gaagcgattc
      841 cgaacgatga acgtgatacc accatgccgg cagcaatggc gaccaccctg cgtaaactgc
      901 tgacgggtga gctgctgacc ctggcaagcc gccagcaact gattgattgg atggaagcgg
      961 ataaagtggc gggtccgctg ctgcgtagcg cgctgccggc tggctggttt attgcggata
     1021 aaagcggtgc gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc ccggatggta
     1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta
     1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta
     1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg
     1261 tctcgtacaa ttctgaggtc cacacagata gtgtcctgtt ctacttttgt ttgttagtct
     1321 tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg tatttagcca
     1381 gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat
     1441 catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg
     1501 cagttaaagc atcgtgtagt gtttttctta gtccgttacg taggtaggaa tctgatgtaa
     1561 tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga
     1621 gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct
     1681 tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa
     1741 cccattggtt aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa
     1801 attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta
     1861 ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat
     1921 tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta
     1981 attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc tcaaagcctt
     2041 taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta
     2101 atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg ttataagtga
     2161 acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac
     2221 agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat
     2281 ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac
     2341 tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt tgagttgtgg
     2401 gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg
     2461 taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata
     2521 gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac
     2581 tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac
     2641 aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg gttgcggccg
     2701 caatcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     2761 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     2821 gcactacagg cgcggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag
     2881 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
     2941 ttatttgatg cctttaatta aggctcggga gacctatcgg taataacagt ccaatctggt
     3001 gtaacttcgg aatcgtcccc aattattgaa cacccttcgg ggtgtttttt tgtttctggt
     3061 ctaccatctc gttgtgataa tagacctgaa gtgcctactc tggaaaatct ttgacagcta
     3121 gctcagtcct aggtataatg ctagcagctg tcaccggatg tgctttccgg tctgatgagt
     3181 ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa ggctcgctac gagaagattg
     3241 ttactttcgc agcgttatta tctaaggagg tagtccatgg ttagcaaagg cgaggcggtt
     3301 atcaaggagt ttatgcgttt taaggttcac atggagggta gcatgaatgg tcacgagttc
     3361 gagatcgagg gtgaaggcga gggtcgtccg tacgaaggca cccagaccgc gaagctgaaa
     3421 gtgaccaagg gtggcccgct gccgttcagc tgggacatcc tgagcccgca gttcatgtat
     3481 ggcagccgtg cgtttaccaa acacccggcg gacattccgg attactataa gcaaagcttc
     3541 ccggaaggtt ttaaatggga gcgtgttatg aacttcgaag atggtggcgc ggtgaccgtt
     3601 acccaggaca ccagcctgga ggatggcacc ctgatttaca aggtgaaact gcgtggcacc
     3661 aactttccgc cggatggtcc ggttatgcag aagaaaacga tgggttggga agcgagcacc
     3721 gagcgtctgt atccggaaga tggcgtgctg aagggtgata tcaaaatggc gctgcgtctg
     3781 aaggacggtg gccgttacct ggcggatttt aagaccacct ataaagcgaa gaaaccggtg
     3841 caaatgccgg gtgcgtacaa cgttgaccgt aaactggata ttaccagcca caacgaggat
     3901 tataccgtgg ttgagcaata tgagcgtagc gagggtcgcc acagcaccgg cggcatggac
     3961 gaactgtata agggatccta ataa
//
