LOCUS       sequence-38385-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Tabor JJ, Levskaya A, Voigt CA
  TITLE     Multichromatic control of gene expression in Escherichia coli.
  JOURNAL   J Mol Biol. 2011 Jan 14. 405(2):315-24.
  PUBMED    21035461
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Jan 14. 405(2):315-24."
COMMENT     Sequence Label: pJT106b3
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     158..180
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    328..349
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      352..395
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(399..417)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(805..824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcacgcttct ggtgccggaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt
       61 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      121 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      181 acgggatcta tcatggcgcg gcgttaggat gtcctgggga acagtgtgtt atcaccgcca
      241 gaggtaaaat agtcaacacg cacggtgtta ttcgactata acacaccatt ttcttgttgt
      301 caacctgtac gatcctacag gtgcttactc tagaagcggc cgcgaattcc agaaatcatc
      361 cttagcgaaa gctaaggatt ttttttatct gaaattctgc ctcgtgatac gcctattttt
      421 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcacca tgggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      721 cgaactggat ctcaacagcg gtaagatccn tgagagtttt cgccccgaag aacgttttcc
      781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      841 gcaagagcaa ctcggtcgcg c
//
