LOCUS       sequence-383853-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral BFP expression vector bearing a sgRNA targeting the 
            promoter of human T-REX17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu 
            HJ, Mackowiak SD, Braendl B, Giesselmann P, Tornisiello R, Parsi KM,
            Huey J, Mielke T, Meierhofer D, Maehr R, Hnisz D, Michor F, Rinn JL,
            Meissner A
  TITLE     T-REX17 is a transiently expressed non-coding RNA essential for 
            human endoderm formation.
  JOURNAL   Elife. 2023 Jan 31;12:e83077. doi: 10.7554/eLife.83077.
  PUBMED    36719724
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-01-31"; volume: "12:e83077. doi"; pages: " 
            10.7554/eLife.83077"
COMMENT     Sequence Label: pU6-sgT-REX17_EF1a-Puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(132..151)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        323..702
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        704..902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     857..877
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             920..1100
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1147..1272
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1769..2002
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2187..2231
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2529..2646
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2899..2932)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2936..2956
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        3201..3412
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3413..4351
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4308..4328
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4372..4968
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4372..4391)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4868..4888
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4978..5031
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5044..5742
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(5762..5795)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5851..6439
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5904..5924)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6322..6333)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6442..6458)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6443..6459)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6968..7148
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7210..7798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7290..7309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7969..8760,8761..8829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8592..8611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8830..8877,1..57))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
       61 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
      121 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
      181 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
      241 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
      301 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      901 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
      961 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     1021 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     1081 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     1141 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     1201 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     1261 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     1321 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     1381 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     1441 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     1501 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     1561 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     1621 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1681 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1741 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1801 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1861 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1921 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1981 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     2041 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     2101 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     2161 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     2221 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     2281 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2341 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2401 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2461 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2521 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2581 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2641 aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc gggcccgctc
     2701 tagagatccg acgcgccatc tctaggcccg cgccggcccc ctcgcacgga cttgtgggag
     2761 aagctcggct actcccctgc cccggttaat ttgcatataa tatttcctag taactataga
     2821 ggcttaatgt gcgataaaag acagataatc tgttcttttt aatactagct acattttaca
     2881 tgataggctt ggatttctat aacttcgtat agcatacatt atacgaagtt ataaacagca
     2941 caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aagtatccct
     3001 tggagaacca ccttgttgag tggtgtggat ttcggcaggt ttaagagcta tgctggaaac
     3061 agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     3121 gtgctttttt tctcgagtac taggatccat taggcggccg cgtggataac cgtattaccg
     3181 ccatgcatgt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     3241 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     3301 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     3361 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     3421 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     3481 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     3541 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     3601 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     3661 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     3721 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     3781 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     3841 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     3901 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     3961 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     4021 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     4081 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     4141 ttagttctcg cgcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     4201 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     4261 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     4321 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gctagcccac catgaccgag
     4381 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc
     4441 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc
     4501 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     4561 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     4621 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     4681 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     4741 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     4801 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     4861 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     4921 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atcgggagag
     4981 ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc accggtcgcc
     5041 accagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     5101 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     5161 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     5221 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     5281 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     5341 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     5401 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     5461 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     5521 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
     5581 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     5641 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     5701 tactgcgacc tccctagcaa actggggcac aagcttaatt aagaattcgt cgagggacct
     5761 aataacttcg tatagcatac attatacgaa gttatacatg tttaagggtt ccggttccac
     5821 taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     5881 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5941 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6001 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6061 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6121 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6181 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6241 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6301 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6361 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6421 ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg
     6481 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     6541 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     6601 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6661 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     6721 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     6781 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     6841 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     6901 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     6961 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7021 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7081 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7141 ctctagcagc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7201 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7261 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7321 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7381 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7441 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7501 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7561 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7621 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7681 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7741 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7801 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7861 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7921 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7981 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8041 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8101 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8161 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8221 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8281 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8341 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8401 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8461 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8521 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8581 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8641 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8701 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8761 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8821 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtct
//
