LOCUS       sequence-383854-        8879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral BFP expression vector bearing a sgRNA targeting a 
            randomized human TSS sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu 
            HJ, Mackowiak SD, Braendl B, Giesselmann P, Tornisiello R, Parsi KM,
            Huey J, Mielke T, Meierhofer D, Maehr R, Hnisz D, Michor F, Rinn JL,
            Meissner A
  TITLE     T-REX17 is a transiently expressed non-coding RNA essential for 
            human endoderm formation.
  JOURNAL   Elife. 2023 Jan 31;12:e83077. doi: 10.7554/eLife.83077.
  PUBMED    36719724
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-01-31"; volume: "12:e83077. doi"; pages: " 
            10.7554/eLife.83077"
COMMENT     Sequence Label: pU6-sgCtrl_EF1a-Puro-T2A-BFP
FEATURES             Location/Qualifiers
     source          1..8879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             16..196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(258..846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(338..357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1017..1808,1809..1877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1640..1659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1878..1982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2057..2076)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2248..2627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2629..2827
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2782..2802
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2845..3025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3072..3197
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3694..3927
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4112..4156
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4454..4571
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(4824..4857)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4861..4881
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        5128..5339
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5340..6278
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6235..6255
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6299..6895
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6299..6318)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6795..6815
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6905..6958
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6971..7669
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(7689..7722)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7778..8366
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7831..7851)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8249..8260)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8369..8385)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8370..8386)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
       61 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      121 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      181 tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1981 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     2041 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     2101 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     2161 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     2221 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     2281 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2341 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2401 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2461 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2521 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2581 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     2641 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     2701 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2761 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2821 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     2881 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     2941 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3001 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     3061 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     3121 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     3181 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     3241 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     3301 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     3361 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     3421 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     3481 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     3541 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     3601 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     3661 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     3721 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     3781 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     3841 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     3901 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     3961 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     4021 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     4081 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     4141 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     4201 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     4261 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     4321 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     4381 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     4441 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     4501 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     4561 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta gtaccgggcc
     4621 cgctctagag atccgacgcg ccatctctag gcccgcgccg gccccctcgc acggacttgt
     4681 gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt cctagtaact
     4741 atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac tagctacatt
     4801 ttacatgata ggcttggatt tctataactt cgtatagcat acattatacg aagttataaa
     4861 cagcacaaaa ggaaactcac cctaactgta aagtaattgt gtgttttgag actataagta
     4921 tcccttggag aaccaccttg ttggtgcgat gggggggtgg gtagcgttta agagctatgc
     4981 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     5041 cgagtcggtg ctttttttct cgagtactag gatccattag gcggccgcgt ggataaccgt
     5101 attaccgcca tgcatgtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     5161 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     5221 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     5281 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     5341 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     5401 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     5461 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     5521 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     5581 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     5641 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     5701 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     5761 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     5821 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     5881 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     5941 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     6001 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     6061 acctcgatta gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     6121 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     6181 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     6241 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agcccaccat
     6301 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     6361 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     6421 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6481 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6541 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6601 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6661 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6721 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6781 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6841 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc
     6901 gggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccacc
     6961 ggtcgccacc agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     7021 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     7081 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     7141 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     7201 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     7261 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     7321 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     7381 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     7441 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata
     7501 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     7561 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     7621 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattaag aattcgtcga
     7681 gggacctaat aacttcgtat agcatacatt atacgaagtt atacatgttt aagggttccg
     7741 gttccactag gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     7801 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7861 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7921 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7981 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8041 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8101 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8161 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8221 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8281 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8341 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa
     8401 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     8461 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     8521 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8581 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     8641 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     8701 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     8761 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     8821 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctg
//
