LOCUS       sequence-383857-        1464 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of HA-tagged AP-1 u1A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Guo X, Mattera R, Ren X, Chen Y, Retamal C, Gonzalez A, Bonifacino 
            JS
  TITLE     The adaptor protein-1 mu1B subunit expands the repertoire of 
            basolateral sorting signal recognition in epithelial cells.
  JOURNAL   Dev Cell. 2013 Nov 11;27(3):353-66. doi: 
            10.1016/j.devcel.2013.10.006.
  PUBMED    24229647
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.10"; journalName: "Dev Cell"; date: 
            "2013-11-11- 11"; volume: "27"; issue: "3"; pages: "353-66"
COMMENT     Sequence Label: pCI-neo-u1A-(HA)3
FEATURES             Location/Qualifiers
     source          1..1464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1342..1368
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1375..1401
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1405..1431
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttaatacgac tcactatagg ctagcctcga gaattcgcca ccatgtccgc cagcgccgtc
       61 tacgtactgg atctgaaggg caaggtgctc atctgcagga actaccgtgg ggatgtggac
      121 atgtcagagg tggagcactt catgcccatt ctgatggaga aggaggagga gggcatgctg
      181 tcacctatct tggcccatgg tggcgttcgt ttcatgtgga ttaagcacaa caacctgtac
      241 ctggtcgcca cttcaaaaaa gaatgcttgt gtgtcactgg tgttctcctt cctctacaag
      301 gtggtacagg tcttctccga gtactttaag gagttggagg aggagagcat ccgagacaac
      361 tttgtcatca tctacgagct gctagatgag ctcatggact ttggctaccc gcagaccact
      421 gacagcaaga tcttgcagga gtacatcact caggaaggcc acaagctgga aacgggggcc
      481 cctaggcccc cagccacagt caccaatgct gtgtcctggc gttcagaagg catcaagtat
      541 cggaagaatg aagtattcct ggatgtcatt gaggctgtta acctcttggt cagtgccaat
      601 ggcaacgtgc tgcgcagtga gattgtgggt tccatcaaga tgcgggtctt cctctcaggc
      661 atgcctgagt tacgcctggg tctcaatgac aaggtcctct tcgacaacac aggccgaggg
      721 aagagcaagt cagtggagct ggaggatgtg aaattccacc agtgtgtgcg gctgtcacgt
      781 tttgagaacg accgcactat ctccttcatc ccacccgacg gagagtttga actcatgtcc
      841 taccgcctca acacccatgt gaagcctttg atctggattg agtccgtgat tgagaagcat
      901 tcccacagcc gcattgagta catggtcaag gccaagagcc agttcaagag gcggtcaaca
      961 gccaacaatg tagagatcca tataccagtc cccaacgatg ctgattcacc caagttcaag
     1021 actacagtgg ggagtgtcaa gtgggtccct gaaaacagtg agatcgtgtg gtccgtcaag
     1081 tcctttccgg gtggcaagga gtacctgatg cgggcccact ttggccttcc cagtgtggaa
     1141 gctgaagaca aggagggaaa gccccccatc agcgtcaagt ttgagatccc ctacttcact
     1201 acctctggca tccaggtgcg ctacctgaaa atcattgaga agagtgggta ccaggccctg
     1261 ccctgggtac gatatatcac acagaacgga gattatcagc tccggaccca gggatctggt
     1321 tcgggaggct ctggatcagg ttacccatac gatgttccag attacgctgt cgactatccc
     1381 tatgacgtcc cggactatgc cggctaccct tacgacgttc cagattacgc ttgagcggcc
     1441 gcttcccttt agtgagggtt aatg
//
