LOCUS       sequence-383874-        5362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Touchette MH, Van Vlack ER, Bai L, Kim J, Cognetta AB 3rd, Previti 
            ML, Backus KM, Martin DW, Cravatt BF, Seeliger JC
  TITLE     A Screen for Protein-Protein Interactions in Live Mycobacteria 
            Reveals a Functional Link between the Virulence-Associated Lipid 
            Transporter LprG and the Mycolyltransferase Antigen 85A.
  JOURNAL   ACS Infect Dis. 2017 May 12;3(5):336-348. doi: 
            10.1021/acsinfecdis.6b00179. Epub 2017 Mar 21.
  PUBMED    28276676
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsinfecdis.6b00179"; journalName: "ACS Infect Dis"; date: 
            "2017-05-12- 12"; volume: "3"; issue: "5"; pages: "336-348"
COMMENT     Sequence Label: pMV361_MjTyrRS
FEATURES             Location/Qualifiers
     source          1..5362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1384..1430
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1580..1599)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1591..2406
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1664..1683)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2737..3325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3226..3245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagacggtg accacaacgc gcccgctttg atcggggacg tctgcggccg accatttacg
       61 ggtcttgttg tcgttggcgg tcatgggccg aacatactca cccggatcgg agggccgagg
      121 acaaggtcga acgaggggca tgacccggtg cggggcttct tgcactcggc ataggcgagt
      181 gctaagaata acgttggcac tcgcgaccgg tgagtgctag gtcgggacgg tgaggccagg
      241 cccgtcgtcg cagcgagtgg cagcgaggac aacttgagcc gtccgtcgcg ggcactgcgc
      301 ccggccagcg taagtagcgg ggttgccgtc acccggtgac ccccgtttca tccccgatcc
      361 ggaggaatca cttcgcaatg gccaagacaa ttgcggatcc agctgcagaa ttcgacgaat
      421 ttgaaatgat aaagagaaac acatctgaaa ttatcagcga ggaagagtta agagaggttt
      481 taaaaaaaga tgaaaaatct gctggtatag gttttgaacc aagtggtaaa atacatttag
      541 ggcattatct ccaaataaaa aagatgattg atttacaaaa tgctggattt gatataatta
      601 tattgttggc tgatttacac gcctatttaa accagaaagg agagttggat gagattagaa
      661 aaataggaga ttataacaaa aaagtttttg aagcaatggg gttaaaggca aaatatcttt
      721 atggaagtcc tttccagctt gataaggatt atacactgaa tgtctataga ttggctttaa
      781 aaactacctt aaaaagagca agaaggagta tggaacttat agcaagagag gatgaaaatc
      841 caaaggttgc tgaagttatc tatccaataa tgcaggttaa tacgagtcat tatctgggcg
      901 ttgatgttgc agttggaggg atggagcaga gaaaaataca catgttagca agggagcttt
      961 taccaaaaaa ggttgtttgt attcacaacc ctgtcttaac gggtttggat ggagaaggaa
     1021 agatgagttc ttcaaaaggg aattttatag ctgttgatga ctctccagaa gagattaggg
     1081 ctaagataaa gaaagcatac tgcccagctg gagttgttga aggaaatcca ataatggaga
     1141 tagctaaata cttccttgaa tatcctttaa ccataaaaag gccagaaaaa tttggtggag
     1201 atttgacagt taatagctat gaggagttag agagtttatt taaaaataag gaattgcatc
     1261 caatggattt aaaaaatgct gtagctgaag aacttataaa gattttagag ccaattagaa
     1321 agagattata aaagcttatc gatgtcgacg tagttaacta gcgtacgatc gactgccagg
     1381 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat gccatcatgg
     1441 ccgcggtgat cagctagcca cctgacgtcg gggggggggg aaagccacgt tgtgtctcaa
     1501 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1561 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
     1621 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1681 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc ccatgcgcca
     1741 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1801 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1861 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
     1921 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1981 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2041 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2101 aatggctggc ctgttgaaca agtctggaaa gaaatgcata atcttttgcc attctcaccg
     2161 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2221 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2281 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2341 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2401 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2461 gacggcggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc
     2521 ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca
     2581 cctacaacaa agctctcacc aaccgtggct ccctcacttt ctggctggat gatggggcga
     2641 ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcac tagttccatg
     2701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2881 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3121 gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt
     3181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa
     3481 cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc accatctctg
     3541 cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac agggcgaacg
     3601 ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt
     3661 ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag
     3721 gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat gcgttgcaac
     3781 cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa agaaaaatgg
     3841 ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga acccgcgtag
     3901 ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat aggtttttac
     3961 cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag
     4021 cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga ggagacctag
     4081 ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat ccaagcctca
     4141 tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga caacaagatg
     4201 gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac ctggacccct
     4261 ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga gtacacccgg
     4321 aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag cgggcacgcg
     4381 gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac gccagctctg
     4441 gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg gcatgcctac
     4501 aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc agagaacccg
     4561 tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac gcctgaggag
     4621 ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata catcctggcg
     4681 tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat cgtggacgac
     4741 ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc
     4801 gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg
     4861 gagatgatcc gagcgcacat gaaggaccgt acgaagatga acaagggccc cgaggcattc
     4921 ctggtgacca cgacgcaggg caaccggctg tcgaagtccg cgttcaccaa gtcgctgaag
     4981 cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc acgacctccg cgctgtcggc
     5041 gctacgttcg ccgctcaggc aggtgcgacg accaaggagc tgatggcccg tctcggtcac
     5101 acgactccta ggatggcgat gaagtaccag atggcgtctg aggcccgcga cgaggctatc
     5161 gctgaggcga tgtccaagct ggccaagacc tcctgaaacg caaaaagccc ccctcccaag
     5221 gacactgagt cctaaagagg ggggtttctt gtcagtacgc gaagaaccac gcctggccgc
     5281 gagcgccagc accgccgctc tgtgcggaga cctgggcacc agccccgccg ccgccaggag
     5341 cattgccgtt cccgccagaa at
//
