LOCUS       sequence-383883-        7581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 N_R203K+G204R in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 N_R203K+G204R
FEATURES             Location/Qualifiers
     source          1..7581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..69)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(53..69)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(66..88)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    77..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(101..107,108..125,126..131))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    146..167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(284..301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(455..1043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(535..554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1214..2005,2006..2074))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1837..1856
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2075..2179)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2254..2273)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2445..2824
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2825..3028
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2979..2999
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3030..3048
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3051..3069
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3079..3103
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3246..3270
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3459..3470)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4528..4552)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4599..4622
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4659..4682
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4713..4739
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4713..4739
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(4740..5087,5088..5090,5091..5699)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(5743..5760)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5749..5973
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6106..6125)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6257..6304
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6312..7334
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6312..7334
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    join(7464..7581,1..4)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7501..7520)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7555..7574
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
       61 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      121 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      181 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      241 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      301 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      361 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      421 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      481 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      541 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      601 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      661 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      721 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      781 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      841 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      901 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      961 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1021 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1501 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1801 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2041 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2101 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     2221 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2281 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     2341 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     2401 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     2461 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     2521 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2581 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2641 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2701 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     2761 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     2821 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     2881 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     2941 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3001 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
     3061 tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
     3121 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt
     3181 taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga attctgcaga
     3241 tatcaacaag tttgtacaaa aaagttggca tgtccgacaa cggtccccag aaccagcgta
     3301 acgctccaag gatcaccttc ggtggaccta gcgactccac cggcagcaac cagaacggag
     3361 aacgctccgg agccagaagc aagcagcgta ggccacaggg cctgcctaac aacaccgcca
     3421 gctggttcac cgctctgacc cagcacggaa aggaagacct gaagttccca aggggacagg
     3481 gcgtgccaat caacaccaac agctctccag acgaccagat cggatactac aggcgtgcca
     3541 ccaggcgcat cagaggaggc gacggaaaga tgaaggacct gtctcctagg tggtacttct
     3601 actacctggg caccggacca gaggctggtc tgccatacgg cgccaacaag gacggcatca
     3661 tctgggtggc taccgaggga gctctgaaca ccccaaagga ccacatcgga acccgtaacc
     3721 ctgccaacaa cgctgccatc gtgctgcagc tgccacaggg aaccaccctg cccaagggat
     3781 tctacgctga gggctccagg ggtggaagcc aggctagctc tcgttcttcc agccgttcta
     3841 ggaacagctc ccgcaactct accccaggaa gctccaaaag gaccagccca gctcgtatgg
     3901 ccggcaacgg aggtgacgcc gctctggctc tgctgctgct ggaccgtctg aaccagctgg
     3961 aaagcaagat gtccggaaag ggtcagcagc agcagggcca gaccgtgacc aagaagtctg
     4021 ccgctgaggc ctctaagaag cccaggcaga agaggaccgc taccaaggcc tacaacgtga
     4081 cccaggcctt cggaagacgt ggacctgaac agacccaggg aaacttcggc gaccaggagc
     4141 tgatccgcca gggaaccgac tacaagcact ggccacagat cgctcagttc gccccttctg
     4201 ctagcgcctt cttcggaatg tccaggatcg gaatggaggt gaccccttcc ggcacctggc
     4261 tgacctacac cggagctatc aagctggacg acaaggaccc caacttcaag gaccaggtga
     4321 tcctgctgaa caagcacatc gacgcctaca agaccttccc acctaccgag ccaaagaagg
     4381 acaagaagaa gaaggccgac gaaacccagg ccctgccaca gaggcagaag aagcagcaga
     4441 ccgtgaccct gctgccagct gctgacctgg acgacttctc caagcagctg cagcagtcta
     4501 tgagctctgc tgactccacc caggcttacc caactttctt gtacaaagtg gttgatatca
     4561 aggccgccga catcaccagc ctgtacaaga aagtggggtg gagccatccc cagttcgaga
     4621 agggcggagg atctggcggc ggaagcggcg gaggaagctg gtcccaccct cagtttgaga
     4681 aaggcaccga gctgggcagc accatggcca gctaccccta cgacgtgccc gactacgcca
     4741 aggacaacac cgtgcccctg aagctgatcg ccctgctggc caacggcgag ttccactctg
     4801 gcgagcagct gggcgagaca ctgggcatga gcagagccgc catcaacaag cacatccaga
     4861 ccctgcggga ctggggcgtg gacgtgttca cagtgcccgg caagggctac agcctgcccg
     4921 agcccatcca gctgctgaac gccaagcaga tcctgggcca gctggatggc ggatctgtgg
     4981 ccgtgctgcc cgtgatcgac agcaccaacc agtatctgct cgaccggatc ggcgagctga
     5041 agtccggcga cgcctgtatc gccgagtacc agcaggccgg cagaggcggc aggggcagaa
     5101 agtggttcag ccccttcggc gccaacctgt acctgagcat gttctggaga ctggaacagg
     5161 gcccagccgc cgctattggc ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc
     5221 ggaagctggg cgccgacaaa gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc
     5281 ggaagctggc cggcatcctg gtggaactga ccggcaaaac aggcgacgcc gcccagatcg
     5341 tgatcggcgc tggcatcaac atggccatgc ggcgggtgga agagagcgtg gtcaaccagg
     5401 gctggatcac cctgcaggaa gccggcatca acctggaccg gaacaccctg gccgccatgc
     5461 tgatcagaga gctgagagcc gccctggaac tgttcgagca ggaaggcctg gccccctacc
     5521 tgagcagatg ggagaagctg gacaacttca tcaaccggcc cgtgaaactg atcatcggcg
     5581 acaaagagat cttcggcatc agccggggca tcgacaagca gggggccctg ctcctggaac
     5641 aggacggcat catcaagccc tggatgggcg gcgagatcag cctgcggagc gccgagaagt
     5701 gatgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     5761 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5821 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5881 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5941 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6001 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6061 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6121 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6181 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6241 atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     6301 cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     6361 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     6421 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     6481 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     6541 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     6601 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     6661 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     6721 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     6781 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     6841 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     6901 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     6961 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     7021 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     7081 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     7141 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     7201 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     7261 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     7321 gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     7381 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7441 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7501 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7561 tgtccaaact catcaatgta t
//
