LOCUS       sequence-383884-        7581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 N_R203M in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 N_R203M
FEATURES             Location/Qualifiers
     source          1..7581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(136..724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(216..235)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(895..1686,1687..1755))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1518..1537
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1756..1860)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1935..1954)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2126..2505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2506..2709
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2660..2680
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2711..2729
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2732..2750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2760..2784
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2927..2951
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3140..3151)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4209..4233)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4280..4303
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4340..4363
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4394..4420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4394..4420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(4421..4768,4769..4771,4772..5380)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(5424..5441)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5430..5654
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5787..5806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5938..5985
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5993..7015
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5993..7015
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7145..7266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7182..7201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7236..7255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7315..7331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7315..7331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7328..7350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7339..7355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7363..7369,7370..7387,7388..7393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7408..7429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7546..7563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
       61 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      121 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      181 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      241 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      361 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      421 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      481 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      541 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      601 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      661 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      721 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      781 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      841 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      901 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
      961 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1021 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1081 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1141 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1201 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1261 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1321 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1381 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1441 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1501 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1561 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1621 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1681 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1741 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1801 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1861 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     1921 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     1981 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     2041 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     2101 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     2161 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2221 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2281 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2341 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2401 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2461 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2521 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2581 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2641 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2701 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
     2761 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     2821 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
     2881 gagctcggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
     2941 aaaagttggc atgtccgaca acggtcccca gaaccagcgt aacgctccaa ggatcacctt
     3001 cggtggacct agcgactcca ccggcagcaa ccagaacgga gaacgctccg gagccagaag
     3061 caagcagcgt aggccacagg gcctgcctaa caacaccgcc agctggttca ccgctctgac
     3121 ccagcacgga aaggaagacc tgaagttccc aaggggacag ggcgtgccaa tcaacaccaa
     3181 cagctctcca gacgaccaga tcggatacta caggcgtgcc accaggcgca tcagaggagg
     3241 cgacggaaag atgaaggacc tgtctcctag gtggtacttc tactacctgg gcaccggacc
     3301 agaggctggt ctgccatacg gcgccaacaa ggacggcatc atctgggtgg ctaccgaggg
     3361 agctctgaac accccaaagg accacatcgg aacccgtaac cctgccaaca acgctgccat
     3421 cgtgctgcag ctgccacagg gaaccaccct gcccaaggga ttctacgctg agggctccag
     3481 gggtggaagc caggctagct ctcgttcttc cagccgttct aggaacagct cccgcaactc
     3541 taccccagga agctccatgg gcaccagccc agctcgtatg gccggcaacg gaggtgacgc
     3601 cgctctggct ctgctgctgc tggaccgtct gaaccagctg gaaagcaaga tgtccggaaa
     3661 gggtcagcag cagcagggcc agaccgtgac caagaagtct gccgctgagg cctctaagaa
     3721 gcccaggcag aagaggaccg ctaccaaggc ctacaacgtg acccaggcct tcggaagacg
     3781 tggacctgaa cagacccagg gaaacttcgg cgaccaggag ctgatccgcc agggaaccga
     3841 ctacaagcac tggccacaga tcgctcagtt cgccccttct gctagcgcct tcttcggaat
     3901 gtccaggatc ggaatggagg tgaccccttc cggcacctgg ctgacctaca ccggagctat
     3961 caagctggac gacaaggacc ccaacttcaa ggaccaggtg atcctgctga acaagcacat
     4021 cgacgcctac aagaccttcc cacctaccga gccaaagaag gacaagaaga agaaggccga
     4081 cgaaacccag gccctgccac agaggcagaa gaagcagcag accgtgaccc tgctgccagc
     4141 tgctgacctg gacgacttct ccaagcagct gcagcagtct atgagctctg ctgactccac
     4201 ccaggcttac ccaactttct tgtacaaagt ggttgatatc aaggccgccg acatcaccag
     4261 cctgtacaag aaagtggggt ggagccatcc ccagttcgag aagggcggag gatctggcgg
     4321 cggaagcggc ggaggaagct ggtcccaccc tcagtttgag aaaggcaccg agctgggcag
     4381 caccatggcc agctacccct acgacgtgcc cgactacgcc aaggacaaca ccgtgcccct
     4441 gaagctgatc gccctgctgg ccaacggcga gttccactct ggcgagcagc tgggcgagac
     4501 actgggcatg agcagagccg ccatcaacaa gcacatccag accctgcggg actggggcgt
     4561 ggacgtgttc acagtgcccg gcaagggcta cagcctgccc gagcccatcc agctgctgaa
     4621 cgccaagcag atcctgggcc agctggatgg cggatctgtg gccgtgctgc ccgtgatcga
     4681 cagcaccaac cagtatctgc tcgaccggat cggcgagctg aagtccggcg acgcctgtat
     4741 cgccgagtac cagcaggccg gcagaggcgg caggggcaga aagtggttca gccccttcgg
     4801 cgccaacctg tacctgagca tgttctggag actggaacag ggcccagccg ccgctattgg
     4861 cctgagcctg gtcatcggca tcgtgatggc cgaggtgctg cggaagctgg gcgccgacaa
     4921 agtgcgcgtg aagtggccca acgacctgta tctgcaggac cggaagctgg ccggcatcct
     4981 ggtggaactg accggcaaaa caggcgacgc cgcccagatc gtgatcggcg ctggcatcaa
     5041 catggccatg cggcgggtgg aagagagcgt ggtcaaccag ggctggatca ccctgcagga
     5101 agccggcatc aacctggacc ggaacaccct ggccgccatg ctgatcagag agctgagagc
     5161 cgccctggaa ctgttcgagc aggaaggcct ggccccctac ctgagcagat gggagaagct
     5221 ggacaacttc atcaaccggc ccgtgaaact gatcatcggc gacaaagaga tcttcggcat
     5281 cagccggggc atcgacaagc agggggccct gctcctggaa caggacggca tcatcaagcc
     5341 ctggatgggc ggcgagatca gcctgcggag cgccgagaag tgatgactcg agtctagagg
     5401 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     5461 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5521 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5581 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5641 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     5701 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5761 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5821 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5881 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtacctagaa
     5941 gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg ccaaaaagcc
     6001 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     6061 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     6121 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     6181 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     6241 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     6301 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     6361 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     6421 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     6481 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
     6541 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
     6601 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
     6661 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
     6721 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
     6781 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     6841 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
     6901 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
     6961 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt
     7021 actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7081 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7141 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7201 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7261 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7321 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7381 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7441 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7561 gctgcgctcg gtcgttcggc t
//
