LOCUS       sequence-383886-        8472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 NSP13_E341D in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 NSP13_E341D
FEATURES             Location/Qualifiers
     source          1..8472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1800..1819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1991..2370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2371..2574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2525..2545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2576..2594
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2597..2615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2625..2649
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2792..2816
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4965..4989)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5036..5059
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5096..5119
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5150..5176
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5150..5176
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5177..5524,5525..5527,5528..6136)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6180..6197)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6186..6410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6543..6562)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6694..6741
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6749..7771
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6749..7771
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7901..8022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7938..7957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7992..8011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8071..8087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8071..8087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8084..8106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8095..8111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8119..8125,8126..8143,8144..8149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8164..8185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8302..8319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     1801 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     1861 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     1921 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     1981 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2041 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2101 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2161 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2221 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2281 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2341 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2401 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2461 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2521 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct
     2581 atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac
     2641 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     2701 cgatccagcc tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact
     2761 agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ttggcatggc
     2821 cgtgggcgct tgcgtgctgt gcaactctca gaccagcctg agatgtggag cttgcatccg
     2881 caggcctttc ctgtgctgca agtgctgcta cgaccacgtg atcagcacct cccacaagct
     2941 ggtgctgtcc gtgaacccat acgtgtgcaa cgctcctggt tgtgacgtga ctgacgtgac
     3001 ccagctgtac ctgggcggaa tgagctacta ctgcaagtcc cacaagcccc ctatcagctt
     3061 cccactgtgc gctaacggcc aggtgttcgg actgtacaag aacacctgcg tgggctccga
     3121 caacgtgacc gacttcaacg ccatcgctac ctgcgactgg accaacgccg gagactacat
     3181 cctggctaac acctgcaccg aaaggctgaa gctgttcgcc gctgagaccc tgaaggccac
     3241 cgaggaaacc ttcaagctgt cttacggtat cgctaccgtg cgtgaggtgc tgtctgaccg
     3301 cgaactgcac ctgtcttggg aagtgggcaa gccaagacca cccctgaacc gtaactacgt
     3361 gttcaccgga tacagggtga ccaagaacag caaggtgcag atcggagagt acaccttcga
     3421 aaagggagac tacggtgacg ccgtggtgta cagaggaacc accacctaca agctgaacgt
     3481 gggtgactac ttcgtgctga cctcccacac cgtgatgcca ctgtctgctc ccaccctggt
     3541 gcctcaggaa cactacgtgc gcatcaccgg actgtaccca accctgaaca tctccgacga
     3601 gttcagctcc aacgtggcca actaccagaa agtgggcatg cagaagtact ccaccctgca
     3661 gggacctcca ggtaccggca agtctcactt cgccatcggt ctggctctgt actacccctc
     3721 tgcccgtatc gtgtacactg cttgttctca cgctgctgtg gatgctctgt gcgagaaggc
     3781 tctgaagtac ctgcctatcg acaagtgctc ccgcatcatc ccagccagag ctcgtgtgga
     3841 ttgcttcgac aagttcaagg tgaactctac cctggagcag tacgtgttct gcaccgtgaa
     3901 cgccctgcct gaaaccaccg ctgacatcgt ggtgttcgac gagatcagct ccaacgtggc
     3961 caactaccag aaagtgggca tgcagaagta ctccaccctg cagggacctc caggtaccgg
     4021 caagtctcac ttcgccatcg gtctggctct gtactacccc tctgcccgta tcgtgtacac
     4081 tgcttgttct cacgctgctg tggatgctct gtgcgagaag gctctgaagt acctgcctat
     4141 cgacaagtgc tcccgcatca tcccagccag agctcgtgtg gattgcttcg acaagttcaa
     4201 ggtgaactct accctggagc agtacgtgtt ctgcaccgtg aacgccctgc ctgaaaccac
     4261 cgctgacatc gtggtgttcg acgagatcag catggccacc aactacgacc tgtctgtggt
     4321 gaacgctaga ctgagggcta agcactacgt gtacatcggt gacccagctc agctgcctgc
     4381 tcctcgtacc ctgctgacca agggcaccct ggagccagaa tacttcaaca gcgtgtgccg
     4441 cctgatgaag accatcggtc cagacatgtt cctgggtacc tgccgccgct gccctgctga
     4501 aatcgtggac accgtgtctg ctctggtgta cgacaacaag ctgaaggccc acaaggacaa
     4561 gagcgcccag tgcttcaaga tgttctacaa gggagtgatc acccacgacg tgtctagcgc
     4621 catcaacagg ccccagatcg gtgtggtgcg cgagttcctg accaggaacc ccgcctggag
     4681 aaaggccgtg ttcatctccc cttacaactc tcagaacgcc gtggcttcca agatcctggg
     4741 tctgcctacc cagaccgtgg actcctctca gggctctgaa tacgactacg tgatcttcac
     4801 ccagaccacc gagaccgccc acagctgcaa cgtgaacagg ttcaacgtgg ccatcaccag
     4861 agctaaagtg ggaatcctgt gcatcatgag cgacagagac ctgtacgaca agctgcagtt
     4921 cacctccctg gagatccccc gcaggaacgt ggctaccctg cagtacccaa ctttcttgta
     4981 caaagtggtt gatatcaagg ccgccgacat caccagcctg tacaagaaag tggggtggag
     5041 ccatccccag ttcgagaagg gcggaggatc tggcggcgga agcggcggag gaagctggtc
     5101 ccaccctcag tttgagaaag gcaccgagct gggcagcacc atggccagct acccctacga
     5161 cgtgcccgac tacgccaagg acaacaccgt gcccctgaag ctgatcgccc tgctggccaa
     5221 cggcgagttc cactctggcg agcagctggg cgagacactg ggcatgagca gagccgccat
     5281 caacaagcac atccagaccc tgcgggactg gggcgtggac gtgttcacag tgcccggcaa
     5341 gggctacagc ctgcccgagc ccatccagct gctgaacgcc aagcagatcc tgggccagct
     5401 ggatggcgga tctgtggccg tgctgcccgt gatcgacagc accaaccagt atctgctcga
     5461 ccggatcggc gagctgaagt ccggcgacgc ctgtatcgcc gagtaccagc aggccggcag
     5521 aggcggcagg ggcagaaagt ggttcagccc cttcggcgcc aacctgtacc tgagcatgtt
     5581 ctggagactg gaacagggcc cagccgccgc tattggcctg agcctggtca tcggcatcgt
     5641 gatggccgag gtgctgcgga agctgggcgc cgacaaagtg cgcgtgaagt ggcccaacga
     5701 cctgtatctg caggaccgga agctggccgg catcctggtg gaactgaccg gcaaaacagg
     5761 cgacgccgcc cagatcgtga tcggcgctgg catcaacatg gccatgcggc gggtggaaga
     5821 gagcgtggtc aaccagggct ggatcaccct gcaggaagcc ggcatcaacc tggaccggaa
     5881 caccctggcc gccatgctga tcagagagct gagagccgcc ctggaactgt tcgagcagga
     5941 aggcctggcc ccctacctga gcagatggga gaagctggac aacttcatca accggcccgt
     6001 gaaactgatc atcggcgaca aagagatctt cggcatcagc cggggcatcg acaagcaggg
     6061 ggccctgctc ctggaacagg acggcatcat caagccctgg atgggcggcg agatcagcct
     6121 gcggagcgcc gagaagtgat gactcgagtc tagagggccc gtttaaaccc gctgatcagc
     6181 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6241 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6301 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6361 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6421 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6661 aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc
     6721 tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga
     6781 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     6841 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     6901 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     6961 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     7021 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     7081 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     7141 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     7201 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     7261 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     7321 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     7381 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     7441 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     7501 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     7561 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     7621 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     7681 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     7741 ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc
     7801 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7861 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8041 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8461 gttgctggcg tt
//
