LOCUS       sequence-383888-       10065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 NSP3_A890D in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 NSP3_A890D
FEATURES             Location/Qualifiers
     source          1..10065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..34)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    23..247
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(380..399)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    531..578
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             586..1608
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             586..1608
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1738..1859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1775..1794)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1829..1848
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1908..1924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1908..1924)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1921..1943)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1932..1948
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1956..1962,1963..1980,1981..1986))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2001..2022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2139..2156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2310..2898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2390..2409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3069..3860,3861..3929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3692..3711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3930..4034)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4109..4128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4300..4679
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4680..4883
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4834..4854
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4885..4903
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4906..4924
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4934..4958
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5101..5125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8867..8891)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8938..8961
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             8998..9021
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9052..9078
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             9052..9078
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(9079..9426,9427..9429,9430..10038)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
       61 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      121 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
      181 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
      241 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
      301 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      361 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      421 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      481 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta gaagttccta
      541 ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa gcctgaactc
      601 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
      661 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
      721 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
      781 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
      841 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
      901 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
      961 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     1021 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     1081 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     1141 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     1201 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     1261 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     1321 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     1381 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     1441 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     1501 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     1561 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca cgtactacga
     1621 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     1681 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     1741 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1801 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1861 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     1921 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1981 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     2041 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     2101 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2161 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2221 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2281 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2341 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2401 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2461 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2521 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2581 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2641 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2701 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2761 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2821 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2881 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2941 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3001 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3061 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3121 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3181 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3241 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3301 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3361 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3421 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3481 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3541 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3601 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3661 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3721 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3781 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3841 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3901 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3961 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4021 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     4081 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4141 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     4201 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     4261 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     4321 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     4381 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     4441 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     4501 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     4561 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     4621 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     4681 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     4741 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     4801 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     4861 gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata
     4921 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     4981 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac
     5041 ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca
     5101 acaagtttgt acaaaaaagt tggcatggcc cctaccaagg tgaccttcgg agacgacacc
     5161 gtgatcgagg tgcagggtta caagagcgtg aacatcacct tcgagctgga cgaacgcatc
     5221 gacaaggtgc tgaacgaaaa gtgctccgct tacaccgtgg agctgggcac cgaggtgaac
     5281 gaattcgcct gcgtggtggc cgacgctgtg atcaagaccc tgcagccagt gtccgaactg
     5341 ctgacccctc tgggtatcga cctggacgag tggtctatgg ctacctacta cctgttcgac
     5401 gagagcggcg aattcaagct ggcctctcac atgtactgca gcttctaccc tcccgacgaa
     5461 gacgaggaag agggtgactg cgaagaggaa gagttcgagc cttccactca gtacgagtac
     5521 ggcaccgaag acgactacca gggcaagcct ctggaattcg gagccacctc tgccgctctg
     5581 cagcctgaag aggaacagga ggaagactgg ctggacgacg actcccagca gaccgtgggt
     5641 cagcaggacg gctctgaaga caaccagacc accaccatcc agaccatcgt ggaggtgcag
     5701 cctcagctgg agatggaact gaccccagtg gtgcagacca tcgaggtgaa cagcttctcc
     5761 ggctacctga agctgaccga caacgtgtac atcaagaacg ctgacatcgt ggaggaagcc
     5821 aagaaggtga aaccaaccgt ggtggtgaac gccgctaacg tgtacctgaa gcacggcgga
     5881 ggtgtggctg gagctctgaa caaggctacc aacaacgcca tgcaggtgga atccgacgac
     5941 tacatcgcta ccaacggtcc tctgaaggtg ggcggatcct gcgtgctgtc tggccacaac
     6001 ctggccaagc actgcctgca cgtggtggga ccaaacgtga acaagggaga ggacatccag
     6061 ctgctgaaga gcgcctacga aaacttcaac cagcacgagg tgctgctggc tccactgctg
     6121 tctgccggaa tcttcggtgc tgatcccatc cacagcctgc gcgtgtgcgt ggacaccgtg
     6181 aggaccaacg tgtacctggc cgtgttcgac aagaacctgt acgacaagct ggtgagctcc
     6241 ttcctggaga tgaagtccga aaagcaggtg gagcagaaga tcgccgagat ccccaaggag
     6301 gaagtgaagc ctttcatcac cgaatctaag cccagcgtgg agcagagaaa gcaggacgac
     6361 aagaagatca aggcctgcgt ggaggaagtg accaccaccc tggaggaaac caagttcctg
     6421 accgagaacc tgctgctgta catcgacatc aacggcaacc tgcaccctga ctccgctacc
     6481 ctggtgtctg acatcgacat caccttcctg aagaaggacg ccccctacat cgtgggcgac
     6541 gtggtgcagg aaggagtgct gaccgctgtg gtcatcccta ccaagaaggc cggtggcacc
     6601 accgagatgc tggccaaggc tctgcgtaag gtgccaaccg acaactacat caccacctac
     6661 cccggtcagg gcctgaacgg ttacaccgtg gaggaagcta agaccgtgct gaagaagtgc
     6721 aagtctgcct tctacatcct gccctccatc atctctaacg agaagcagga aatcctgggc
     6781 accgtgagct ggaacctgcg cgaaatgctg gcccacgctg aggaaaccag gaagctgatg
     6841 cccgtgtgcg tggagaccaa ggccatcgtg agcaccatcc agagaaagta caagggcatc
     6901 aagatccagg agggtgtggt ggactacggc gctcgtttct acttctacac ctccaagacc
     6961 accgtggcct ctctgatcaa caccctgaac gacctgaacg agaccctggt gaccatgcca
     7021 ctgggatacg tgacccacgg tctgaacctg gaggaagccg ctagatacat gcgttccctg
     7081 aaggtgcctg ctaccgtgag cgtgagcagc cctgacgctg tgaccgccta caacggatac
     7141 ctgacctcct ctagcaagac ccctgaggaa cacttcatcg aaaccatcag cctggccggt
     7201 tcctacaagg actggtctta cagcggacag tccacccagc tgggcatcga gttcctgaag
     7261 cgcggagaca agagcgtgta ctacacctcc aacccaacca ccttccacct ggacggtgaa
     7321 gtgatcacct tcgacaacct gaagaccctg ctgtctctgc gcgaggtgag gaccatcaag
     7381 gtgttcacca ccgtggacaa catcaacctg cacacccagg tggtggacat gagcatgacc
     7441 tacggacagc agttcggtcc tacctacctg gacggagctg acgtgaccaa gatcaagcca
     7501 cacaactccc acgagggcaa gaccttctac gtgctgccta acgatgacac cctgagagtg
     7561 gaggctttcg aatactacca caccaccgac ccatccttcc tgggcaggta catgtctgcc
     7621 ctgaaccaca ccaagaagtg gaagtacccc caggtgaacg gactgaccag catcaagtgg
     7681 gccgacaaca actgctacct ggccaccgct ctgctgaccc tgcagcagat cgaactgaag
     7741 ttcaacccac ccgctctgca ggacgcttac tacagagccc gtgctggcga ggccgataac
     7801 ttctgcgctc tgatcctggc ctactgcaac aagaccgtgg gcgaactggg agacgtgaga
     7861 gagaccatga gctacctgtt ccagcacgcc aacggcggca agggattctg caagctgcac
     7921 aactggaact gcgtgaactg cgacaccttc tgcgctggct ccaccttcat ctctgacgag
     7981 gtggccagag acctgagcct gcagttcaag cgtccaatca accccaccga ccagagctcc
     8041 tacatcgtgg actctgtgac cgtgaagaac ggtagcatcc acctgtactt cgacaaggct
     8101 ggccagaaga cctacgagcg ccactctctg agccacttcg tgaacctgga caacctgagg
     8161 gccaacaaca ccaagggcag cctgcctatc aacgtgatcg tgttcgacgg aaagtccaag
     8221 tgcgaggaat ctagcgccaa gtccgccagc gtgtactaca gccagctgat gtgccagcca
     8281 atcctgctgc tggaccaggc tctggtgtct gacgtgggag acagcgccga agtggccgtg
     8341 aagatgttcg acgcctacgt gaacaccttc tcctctacct tcaacgtgcc tatggagaag
     8401 ctgaagaccc tggtggctac tgctgaggct gaactggcta agaacgtgtc cctggacaac
     8461 gtgctgagca ccttcatctc cgccgctagg cagggtttcg tggactctga cgtggaaacc
     8521 aaagacgtgg tggagtgcct gaagctgtct caccagagcg acatcgaagt gaccggcgac
     8581 tcctgcaaca actacatgct gacctacaac aaggtggaga acatgacccc aagagacctg
     8641 ggagcttgca tcgactgctc cgcccgtcac atcaacgctc aggtggccaa gtctcacaac
     8701 atcgccctga tctggaacgt gaaggacttc atgagcctgt ccgagcagct gcgcaagcag
     8761 atcaggagcg ccgctaagaa gaacaacctg cccttcaagc tgacctgcgc taccaccaga
     8821 caggtggtga acgtggtgac caccaagatc gccctgaagg gcggataccc aactttcttg
     8881 tacaaagtgg ttgatatcaa ggccgccgac atcaccagcc tgtacaagaa agtggggtgg
     8941 agccatcccc agttcgagaa gggcggagga tctggcggcg gaagcggcgg aggaagctgg
     9001 tcccaccctc agtttgagaa aggcaccgag ctgggcagca ccatggccag ctacccctac
     9061 gacgtgcccg actacgccaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc
     9121 aacggcgagt tccactctgg cgagcagctg ggcgagacac tgggcatgag cagagccgcc
     9181 atcaacaagc acatccagac cctgcgggac tggggcgtgg acgtgttcac agtgcccggc
     9241 aagggctaca gcctgcccga gcccatccag ctgctgaacg ccaagcagat cctgggccag
     9301 ctggatggcg gatctgtggc cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc
     9361 gaccggatcg gcgagctgaa gtccggcgac gcctgtatcg ccgagtacca gcaggccggc
     9421 agaggcggca ggggcagaaa gtggttcagc cccttcggcg ccaacctgta cctgagcatg
     9481 ttctggagac tggaacaggg cccagccgcc gctattggcc tgagcctggt catcggcatc
     9541 gtgatggccg aggtgctgcg gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac
     9601 gacctgtatc tgcaggaccg gaagctggcc ggcatcctgg tggaactgac cggcaaaaca
     9661 ggcgacgccg cccagatcgt gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa
     9721 gagagcgtgg tcaaccaggg ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg
     9781 aacaccctgg ccgccatgct gatcagagag ctgagagccg ccctggaact gttcgagcag
     9841 gaaggcctgg ccccctacct gagcagatgg gagaagctgg acaacttcat caaccggccc
     9901 gtgaaactga tcatcggcga caaagagatc ttcggcatca gccggggcat cgacaagcag
     9961 ggggccctgc tcctggaaca ggacggcatc atcaagccct ggatgggcgg cgagatcagc
    10021 ctgcggagcg ccgagaagtg atgactcgag tctagagggc ccgtt
//
