LOCUS       sequence-383893-       11750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector expressing LCB3 SynNotch and mCherry 
            transduction marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11750)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 11750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)
FEATURES             Location/Qualifiers
     source          1..11750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             71..704
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    751..876
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1373..1606
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1791..1835
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2120..2237
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2293..2792
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2328..2351)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2725..2744
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2862..2891
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4098..4538
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4575..4724
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4746..4799
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4809..5519
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4954..4972)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5222..5241
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5534..6122
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5587..5607)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6005..6016)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6144..6160)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6145..6161)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6251..6484
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6509..6527)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6510..6527)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6817..6836)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7050..7069)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7169..7191
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7229..7247)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(7426..8014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7506..7525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8117..8221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8222..8290,8291..9082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8440..8459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     9324..9341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9411..9431)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9416..9745
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9596..9731
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10879..10944
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11074..11094
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11519..11653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11556..11575)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11610..11629
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
       61 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
      121 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
      181 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
      241 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
      301 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
      361 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
      421 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
      481 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggactc tctggttaga
      541 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      601 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      661 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      721 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      781 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      841 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      901 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
      961 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1021 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1081 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1141 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1201 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1261 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1321 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1381 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1441 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1501 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1561 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1621 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1681 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1741 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1801 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1861 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1921 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1981 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2041 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa
     2101 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2161 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2221 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggccctctg
     2281 atgagacgaa cggggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     2341 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     2401 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     2461 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     2521 tggaagtagc atgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     2581 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     2641 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     2701 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     2761 tgttctcctc ttcctcatct ccgggccttt cggccactat ggccttgccc gttacggcac
     2821 ttctgctccc actcgcattg ctgcttcacg ccgcccgacc agaacagaaa ctcataagtg
     2881 aagaagacct tgggggaggc ggttccgggg gaggcggttc taacgatgac gatctccata
     2941 tgcagatgac cgatctggtg tacgaggcac tacactttgc taaagacgag gaaatccaga
     3001 agcacgtctt ccagctgttc gagaaggcca caaaggccta taaaaataaa gacaggcaaa
     3061 aactggaaaa ggttgtggag gaactgaagg aattgttaga gagacttctc agcggttcta
     3121 tcctggacta cagcttcaca ggtggcgctg ggcgcgacat tcccccaccg cagattgagg
     3181 aggcctgtga gctgcctgag tgccaggtgg atgcaggcaa taaggtctgc aacctgcagt
     3241 gtaataatca cgcatgtggc tgggatggtg gcgactgctc cctcaacttc aatgacccct
     3301 ggaagaactg cacgcagtct ctacagtgct ggaagtattt tagcgacggc cactgtgaca
     3361 gccagtgcaa ctcggccggc tgcctctttg atggcttcga ctgccagctc accgagggac
     3421 agtgcaaccc cctgtatgac cagtactgca aggaccactt cagtgatggc cactgcgacc
     3481 agggctgtaa cagtgccgaa tgtgagtggg atggcctaga ctgtgctgag catgtacccg
     3541 agcggctggc agccggcacc ctggtgctgg tggtgctgct tccacccgac cagctacgga
     3601 acaactcctt ccactttctg cgggagctca gccacgtgct gcacaccaac gtggtcttca
     3661 agcgtgatgc gcaaggccag cagatgatct tcccgtacta tggccacgag gaagagctgc
     3721 gcaagcaccc aatcaagcgc tctacagtgg gttgggccac ctcttcactg cttcctggta
     3781 ccagtggtgg gcgccagcgc agggagctgg accccatgga catccgtggc tccattgtct
     3841 acctggagat cgacaaccgg caatgtgtgc agtcatcctc gcagtgcttc cagagtgcca
     3901 ccgatgtggc tgccttccta ggtgctcttg cgtcacttgg cagcctcaat attccttaca
     3961 agattgaggc cgtgaagagt gagccggtgg agcctccgct gccctcgcag ctgcacctca
     4021 tgtacgtggc agcggctgcc ttcgtgctcc tgttctttgt gggctgtggg gtgctgctgt
     4081 cccgcaagcg ccggcggatg aagctgctga gcagcatcga gcaggcctgt gacatctgcc
     4141 ggctgaagaa actgaagtgc agcaaagaaa agcccaagtg cgccaagtgc ctgaagaaca
     4201 actgggagtg ccggtacagc cccaagacca agagaagccc cctgaccaga gcccacctga
     4261 ccgaggtgga aagccggctg gaaagactgg aacagctgtt tctgctgatc ttcccacgcg
     4321 aggacctgga catgatcctg aagatggaca gcctgcagga catcaaggcc ctgctgaccg
     4381 gcctgttcgt gcaggacaac gtgaacaagg acgccgtgac cgacagactg gccagcgtgg
     4441 aaaccgacat gcccctgacc ctgcggcagc acagaatcag cgccaccagc agcagcgagg
     4501 aaagcagcaa caagggccag cggcagctga cagtgtctgc tgctgcaggc ggaagcggag
     4561 gctctggcgg atctgatgcc ctggacgact tcgacctgga tatgctgggc agcgacgccc
     4621 tggatgattt tgatctggac atgctgggat ctgacgctct ggacgatttc gatctcgaca
     4681 tgttgggatc agatgcactg gatgactttg acctggacat gctcggatca ggatccggga
     4741 gtggcgaagg caggggctcc cttttgacgt gcggggatgt tgaagagaac cctgggcccg
     4801 gatctggaat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     4861 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     4921 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     4981 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     5041 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     5101 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     5161 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     5221 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     5281 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     5341 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     5401 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     5461 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaat
     5521 ggcgcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5581 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5641 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5701 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5761 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5821 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5881 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5941 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6001 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6061 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6121 gcatgctgcg tctcaagcat acacgatacc gtcgacctcg agggaattaa ttcgagctcg
     6181 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6241 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6301 actgggtttc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6361 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6421 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6481 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
     6541 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     6601 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     6661 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     6721 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     6781 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     6841 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     6901 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     6961 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     7021 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7081 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     7141 tccgcttaca gacaagctgt gaccatctcc gggagctgca tgtgtcagag gttttcaccg
     7201 tcatcaccga aacgcgcgag atgaaagggc ctcgtgatac gcctattttt ataggttaat
     7261 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgccgagc
     7321 ggccgcacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7861 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga caggtggcac
     8101 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8161 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8221 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8281 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8341 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8401 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8461 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8521 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8581 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8641 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8701 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8761 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8821 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8881 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8941 ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9001 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9061 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9121 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatcgcgg
     9181 ccgccaattt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9241 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9301 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9361 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     9421 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9481 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9541 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9601 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9661 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9721 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     9781 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     9841 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     9901 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     9961 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
    10021 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
    10081 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
    10141 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
    10201 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
    10261 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
    10321 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
    10381 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
    10441 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
    10501 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
    10561 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
    10621 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
    10681 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
    10741 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
    10801 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
    10861 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
    10921 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
    10981 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
    11041 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
    11101 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
    11161 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
    11221 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
    11281 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
    11341 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
    11401 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
    11461 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    11521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    11581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    11641 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    11701 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
//
