LOCUS       sequence-383897-        7449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Thbs1 under control of short GFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Bray ER, Yungher BJ, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe AC, 
            Thakor K, Marks V, Randolph M, Danzi MC, Schmidt TM, Chaudhari N, 
            Lemmon VP, Hattar S, Park KK
  TITLE     Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion 
            Cells.
  JOURNAL   Neuron. 2019 Aug 21;103(4):642-657.e7. doi: 
            10.1016/j.neuron.2019.05.044. Epub 2019 Jun 26.
  PUBMED    31255486
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.05.044"; journalName: "Neuron"; date: 
            "2019-08-21- 21"; volume: "103"; issue: "4"; pages: "642-657.e7"
COMMENT     Sequence Label: pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)
FEATURES             Location/Qualifiers
     source          1..7449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             4498..4524
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     4581..4600
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4712..4852
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4712..4841
                     /label=AAV2 ITR
     rep_origin      4927..5382
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5014..5033)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5224..5245
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5399..5418)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5518..5540
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5578..5596)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5664..5768
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5769..5837,5838..6629)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6800..7388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7289..7308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgctc gtgacctagc aacatatcct ggtgtggagt aggggacgct
      181 gctctgacag aggctcgggg gcctgagctg gctctgtgag cttgggagga ggcagacagc
      241 caggccttgt ctgcaagcag acctggcagc attgggctgg ccgcccccca gggcctcctc
      301 ttcatgccca gtgaatgact caccttggca cagacacaat gttcggggtg ggcacagtgc
      361 ctgcttcccg ccgcacccca gcccccctca aatgccttcc gagaagccca ttgagcaggg
      421 ggcttgcatt gcaccccagc ctgacagcct ggcatcttgg gataaaagca gcacagcccc
      481 ctaggggctg cccttgctgt gtggcgccac cgtcggtgga gaacaaggct ctattcagcc
      541 tgtgcccagg aaaggggatc aggggatgcc caggcatgga cagtgggtgg caggggggga
      601 gaggagggct gtctgcttcc cagaagtcca aggacacaaa tgggtgaggg gagagctctc
      661 cccatagctg ggctgcggcc caaccccacc ccctcaggct atgccagggg gtgttgccag
      721 gggcacccgg gcatcgccag tctatcccac tccttcataa agccctcgca tcccaggagc
      781 gagcagagcc agagcaggtt ggagaggaga cgcatcacct ccgctgctcg cggggtccgg
      841 actctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
      901 tttctctctt ttagattcca acctttggaa ctgaattccg cgggcccggg atcctctaga
      961 gtcgacctgc agaagcttat gaccatggag ctcctgcggg gactaggtgt cctgttcctg
     1021 ttgcatatgt gtggaagcaa ccgcattcca gagtctgggg gagataacgg tgtgtttgac
     1081 atctttgaac tcattggagg tgcacgaagg ggccccggtc gccgactggt gaagggccaa
     1141 gatctatcca gccccgcctt ccgcattgag aatgccaacc tgatccccgc tgtgccggat
     1201 gacaagttcc aagacctact ggacgctgtg tgggccgaca aaggcttcat cttcctggct
     1261 tccttgaggc agatgaagaa gacccggggc acactcctgg ctgtggaacg gaaagacaac
     1321 actggccaga tcttcagtgt ggtctccaac ggcaaagctg gcaccctgga cctgagcctg
     1381 agcctgccag ggaagcaaca agtggtgtca gtggaggaag ctctcctggc cactggccag
     1441 tggaagagca tcacgctgtt tgttcaagag gaccgggctc aactctacat agactgtgat
     1501 aagatggaga gcgcggagct ggatgtaccc atccagagca tcttcaccag ggatctggcc
     1561 agcgttgcca ggctccgagt tgcaaaggga gatgtcaatg acaattttca gggggtgctg
     1621 cagaatgtga ggtttgtctt tggaaccacc ccagaagaca ttctcaggaa caaaggctgc
     1681 tccagctcag ctaccaacgt ccttcttacc cttgacaaca acgtggtgaa cggttccagc
     1741 cctgctatcc gcaccaacta catcggccac aaaacaaagg acctccaagc tatctgtggc
     1801 ctctcctgtg atgaactatc cagcatggtc ctggaactga agggcctgcg caccatcgtg
     1861 accactctgc aggacagcat ccgaaaagtg acggaagaga acagagagct ggtcagtgag
     1921 ctgaagcggc ctcccctctg ctttcacaat ggagtccagt acaagaacaa cgaggagtgg
     1981 actgtagaca gttgcacaga gtgtcactgc cagaactcgg ttaccatctg caaaaaggtg
     2041 tcctgtccca tcatgccctg ctccaacgcc acagttcctg atggtgaatg ctgcccacgg
     2101 tgctggccca gcgactctgc tgacgatggc tggtctccct ggtctgagtg gacctcctgc
     2161 tctgccacat gtggcaatgg aattcagcaa cgtggtcgtt cctgtgacag cctcaacaac
     2221 agatgcgagg gctcttcggt acagacaagg acctgccaca ttcaggagtg tgacaaaaga
     2281 tttaaacagg atggtggctg gagtcactgg tctccatggt cgtcctgttc tgtgacctgt
     2341 ggtgacggtg tgatcacaag gatccggctc tgcaactccc ccagccccca gatgaacggg
     2401 aagccctgtg aaggtgaagc ccgggagacc aaagcctgca agaaagacgc ctgcccaatt
     2461 aatggaggct ggggtccctg gtcaccatgg gacatctgct ctgtcacctg tggaggagga
     2521 gtgcagagac gcagccgact ctgtaacaac cccacacccc agtttggagg caaagactgt
     2581 gttggcgatg tgacagaaaa tcaagtttgc aacaagcagg actgcccaat tgatggatgc
     2641 ctgtccaatc cctgctttgc tggtgccaag tgtactagct accctgatgg tagctggaaa
     2701 tgtggtgcgt gtcctcctgg ctacagtgga aatggcatcc agtgcaaaga cgtcgatgag
     2761 tgcaaagaag tgcctgatgc ttgcttcaat cacaacggag aacatcggtg caagaacaca
     2821 gatcctggct acaactgcct gccctgccca ccacgattca ctggctcaca gcccttcggc
     2881 cgaggtgtcg aacatgccat ggccaacaaa caggtgtgca aaccgcgaaa cccctgcacg
     2941 gacgggacgc atgactgcaa caagaacgct aagtgcaact acctgggtca ctacagtgac
     3001 cccatgtacc gctgtgagtg caagcccggc tatgcaggca atggcatcat ctgcggagag
     3061 gacacagacc tggacggctg gcctaatgaa aacctggtgt gtgtggccaa cgcaacctac
     3121 cactgcaaaa aggacaactg ccccaacctt cccaactcgg ggcaggaaga ctatgacaag
     3181 gacgggattg gcgatgcctg cgatgatgac gatgacaacg acaagatccc cgatgacagg
     3241 gacaactgtc cattccatta caacccagcc cagtatgact atgacagaga tgatgtggga
     3301 gaccgctgtg acaactgccc ctacaaccac aaccctgacc aagcagacac agacaaaaac
     3361 ggggagggcg atgcctgtgc tgtggacatc gatggagatg gaatcctcaa tgaacgagac
     3421 aactgccagt acgtttacaa cgtggaccag agggacacgg acatggatgg ggttggagat
     3481 cagtgtgaca actgccccct ggaacacaat ccagaccagc tggactctga ctcagacctc
     3541 ataggggaca cttgtgacaa caatcaggac atcgatgagg atggccatca gaacaatctg
     3601 gacaactgtc cctatgtgcc taatgccaac caggccgacc atgataaaga tggcaaagga
     3661 gatgcctgtg accatgacga tgacaatgac ggcatccctg atgacagaga caactgcagg
     3721 ctggtgccca atcctgacca gaaggactct gatggtgatg gccgaggtga cgcctgcaaa
     3781 gacgactttg accatgacaa tgtgccagat attgatgaca tctgtcctga gaattttgac
     3841 atcagtgaaa ccgatttccg acaattccag atgattcctc tagatcccaa aggaacctcc
     3901 caaaatgacc ctaactgggt tgtccgccat cagggcaaag aacttgtcca gactgtaaac
     3961 tgtgaccctg gacttgctgt aggttatgat gagtttaatg ctgtggactt cagcggtacc
     4021 ttcttcatca acaccgagag agatgatgac tacgctggct ttgttttcgg ctaccagtcc
     4081 agcagccgct tctacgttgt gatgtggaaa caagtcaccc agtcctactg ggacaccaac
     4141 cccacaaggg ctcagggata ctcaggcctg tctgtaaagg ttgtgaactc caccaccggc
     4201 cctggcgagc acctgcggaa tgcactgtgg cacacaggaa acacccctgg ccaggtgcgc
     4261 accctgtggc atgaccctcg ccacatcggc tggaaagatt tcactgcata cagatggcgt
     4321 ctcagccaca ggccaaagac cggttatatc agagtggtga tgtatgaagg aaagaaaatc
     4381 atggctgact cgggacccat ctatgacaaa acctacgccg gcggtagact aggcctgttc
     4441 gtcttctctc aggaaatggt gttcttctca gacatgaaat acgagtgtcg agattcctac
     4501 ccatacgatg ttccagatta cgcttaactc gagagatcta ggccgctagg cctcacctgc
     4561 gatctcgatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4621 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4681 aggtgtggga ggttttttaa actgcggccg caggaacccc tagtgatgga gttggccact
     4741 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4801 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4861 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4921 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4981 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     5041 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5101 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     5161 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5221 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     5281 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     5341 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     5401 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5461 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5521 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5581 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5641 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5701 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5761 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5821 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5881 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5941 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6001 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6061 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6121 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6181 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6241 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6301 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6361 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6421 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6481 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6541 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6601 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6661 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6721 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6781 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6841 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6901 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6961 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7021 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7081 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7141 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7201 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7261 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7321 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7381 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7441 ctcacatgt
//
