LOCUS       sequence-383898-        6027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CNTF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Yungher BJ, Luo X, Salgueiro Y, Blackmore MG, Park KK
  TITLE     Viral vector-based improvement of optic nerve regeneration: 
            characterization of individual axons' growth patterns and 
            synaptogenesis in a visual target.
  JOURNAL   Gene Ther. 2015 Oct;22(10):811-21. doi: 10.1038/gt.2015.51. Epub 
            2015 May 25.
  PUBMED    26005861
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2015.51"; journalName: "Gene Ther"; date: "2015-10"; 
            volume: "22"; issue: "10"; pages: "811-21"
COMMENT     Sequence Label: pAAV-CNTF
FEATURES             Location/Qualifiers
     source          1..6027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(121..140)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      934..1522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1423..1442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1584..1713
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        1735..2134
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2704..2723
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     ncRNA           3207..3233
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           3297..3338
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     3643..3662
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     polyA_signal    4357..4833
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4556..4575)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4873..5013
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4873..5002
                     /label=AAV2 ITR
     rep_origin      5088..5543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5175..5194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5385..5406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5560..5579)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5679..5701
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5739..5757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5825..5929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5930..5998,5999..6027,1..763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
       61 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      121 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      181 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      241 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      301 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      361 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      421 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      481 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      541 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      601 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
      661 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
      721 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
      781 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
      841 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
      901 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      961 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1021 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1081 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1141 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1201 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1261 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1321 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1381 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1441 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1501 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1561 ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc
     1621 cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
     1681 agggagtggc caactccatc actaggggtt cctgcggccg cacgcgttta aactggcctc
     1741 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     1801 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     1861 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     1921 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     1981 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     2041 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     2101 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     2161 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     2221 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     2281 aatggcggct gttcccgagt cttgaataga accttcgcta atgcgggaaa gctcttattc
     2341 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     2401 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca
     2461 cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
     2521 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
     2581 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     2641 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     2701 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     2761 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     2821 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa gctaaggcgc
     2881 gcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt
     2941 aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg
     3001 tcccttgtat caccatgcat ggaccctcat gataattttg tttctttcac tttctactct
     3061 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     3121 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaaga
     3181 tcccatcgat tcgaattcaa agtcgactgg aggcttgctg aaggctgtat gctgtttatg
     3241 aggatctctc tgattttttt ggcctctgac tgaaaatcaa gagtcctcat aaacaggaca
     3301 caaggcctgt tactagcact cacatggaac aaatggccac cgtgggagga tgacaactcg
     3361 agcctctaga caatcagggt atattatatt gtacttcagc acagttttag agaacaattg
     3421 ttataattaa atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata
     3481 ttcttattgg tagaaacaac tacatcctgg tcatcatcct gcctttctct ttatggttac
     3541 aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg
     3601 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     3661 tgctgtctca tcaagcttaa ggatccaagg taccatgtcc atgttgttct acactctgat
     3721 cacagctttt ctgatcggca tacaggcggc tttcacagag cattcaccgc tgacccctca
     3781 ccgtcgggac ctctgtagcc gctctatctg gctagcaagg aagattcgtt cagacctgac
     3841 tgctcttacg gaatcctatg tgaagcatca gggcctgaac aagaacatca acctggactc
     3901 tgcggatggg atgccagtgg caagcactga tcagtggagt gagctgaccg aggcagagcg
     3961 actccaagag aaccttcaag cttatcgtac cttccatgtt ttgttggcca ggctcttaga
     4021 agaccagcag gtgcatttta ccccaaccga aggtgacttc catcaagcta tacataccct
     4081 tcttctccaa gtcgctgcct ttgcatacca gatagaggag ttaatgatac tcctggaata
     4141 caagatcccc cgcaatgagg ctgatgggat gcctattaat gttggagatg gtggtctctt
     4201 tgagaagaag ctgtggggcc taaaggtgct gcaggagctt tcacagtgga cagtaaggtc
     4261 catccatgac cttcgtttca tttcttctca tcagactggg atcccagcac gtgggagcca
     4321 ttatattgct aacaacaaga aaatgtagag atctacgggt ggcatccctg tgacccctcc
     4381 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     4441 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     4501 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     4561 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     4621 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     4681 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4741 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4801 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     4861 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     4921 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4981 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     5041 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     5101 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5161 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5221 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5281 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     5341 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5401 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     5461 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5521 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     5581 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5641 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5701 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5761 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5821 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5881 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5941 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6001 cccagaaacg ctggtgaaag taaaaga
//
