LOCUS       sequence-383899-       11726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector expressing LCB1 SynNotch and mCherry 
            transduction marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)
FEATURES             Location/Qualifiers
     source          1..11726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             90..723
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    770..895
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1392..1625
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1810..1854
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2139..2256
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2312..2811
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2347..2370)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2744..2763
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2881..2910
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4093..4533
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4570..4719
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4741..4794
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4804..5514
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4949..4967)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5217..5236
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5529..6117
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5582..5602)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6000..6011)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6139..6155)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6140..6156)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6246..6479
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6504..6522)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6505..6522)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6812..6831)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7045..7064)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7164..7186
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7224..7242)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(7421..8009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7501..7520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8112..8216
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8217..8285,8286..9077)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8435..8454)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     9319..9336
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9406..9426)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9411..9740
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9591..9726
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10874..10939
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11069..11089
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11514..11648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11551..11570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11605..11624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
       61 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
      121 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
      181 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
      241 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
      301 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
      361 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
      421 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
      481 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
      541 ctgggactct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      601 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      661 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      721 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      781 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
      841 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
      901 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
      961 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1021 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1081 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1141 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1201 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     1261 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1321 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1381 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1441 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1501 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1561 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1621 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1681 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1741 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1801 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1861 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     1921 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     1981 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     2041 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     2101 tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2161 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2221 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2281 gatccagttt ggccctctga tgagacgaac ggggtagggg aggcgctttt cccaaggcag
     2341 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     2401 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     2461 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     2521 tcgtgcagga cgtgacaaat ggaagtagca tgtctcacta gtctcgtgca gatggacagc
     2581 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     2641 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     2701 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     2761 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc ggccactatg
     2821 gccttgcccg ttacggcact tctgctccca ctcgcattgc tgcttcacgc cgcccgacca
     2881 gaacagaaac tcataagtga agaagacctt gggggaggcg gttccggggg aggcggttct
     2941 gacaaggaaa acattctcca gaaaatatac gagatcatga aaaccctcga gcaactgggc
     3001 cacgcagagg ctagcatgca ggtgtccgat ctgatctatg aattcatgaa gcagggggac
     3061 gagagactgt tggaggaagc cgagcggtta cttgaagagg tcgaaagggg ttctatcctg
     3121 gactacagct tcacaggtgg cgctgggcgc gacattcccc caccgcagat tgaggaggcc
     3181 tgtgagctgc ctgagtgcca ggtggatgca ggcaataagg tctgcaacct gcagtgtaat
     3241 aatcacgcat gtggctggga tggtggcgac tgctccctca acttcaatga cccctggaag
     3301 aactgcacgc agtctctaca gtgctggaag tattttagcg acggccactg tgacagccag
     3361 tgcaactcgg ccggctgcct ctttgatggc ttcgactgcc agctcaccga gggacagtgc
     3421 aaccccctgt atgaccagta ctgcaaggac cacttcagtg atggccactg cgaccagggc
     3481 tgtaacagtg ccgaatgtga gtgggatggc ctagactgtg ctgagcatgt acccgagcgg
     3541 ctggcagccg gcaccctggt gctggtggtg ctgcttccac ccgaccagct acggaacaac
     3601 tccttccact ttctgcggga gctcagccac gtgctgcaca ccaacgtggt cttcaagcgt
     3661 gatgcgcaag gccagcagat gatcttcccg tactatggcc acgaggaaga gctgcgcaag
     3721 cacccaatca agcgctctac agtgggttgg gccacctctt cactgcttcc tggtaccagt
     3781 ggtgggcgcc agcgcaggga gctggacccc atggacatcc gtggctccat tgtctacctg
     3841 gagatcgaca accggcaatg tgtgcagtca tcctcgcagt gcttccagag tgccaccgat
     3901 gtggctgcct tcctaggtgc tcttgcgtca cttggcagcc tcaatattcc ttacaagatt
     3961 gaggccgtga agagtgagcc ggtggagcct ccgctgccct cgcagctgca cctcatgtac
     4021 gtggcagcgg ctgccttcgt gctcctgttc tttgtgggct gtggggtgct gctgtcccgc
     4081 aagcgccggc ggatgaagct gctgagcagc atcgagcagg cctgtgacat ctgccggctg
     4141 aagaaactga agtgcagcaa agaaaagccc aagtgcgcca agtgcctgaa gaacaactgg
     4201 gagtgccggt acagccccaa gaccaagaga agccccctga ccagagccca cctgaccgag
     4261 gtggaaagcc ggctggaaag actggaacag ctgtttctgc tgatcttccc acgcgaggac
     4321 ctggacatga tcctgaagat ggacagcctg caggacatca aggccctgct gaccggcctg
     4381 ttcgtgcagg acaacgtgaa caaggacgcc gtgaccgaca gactggccag cgtggaaacc
     4441 gacatgcccc tgaccctgcg gcagcacaga atcagcgcca ccagcagcag cgaggaaagc
     4501 agcaacaagg gccagcggca gctgacagtg tctgctgctg caggcggaag cggaggctct
     4561 ggcggatctg atgccctgga cgacttcgac ctggatatgc tgggcagcga cgccctggat
     4621 gattttgatc tggacatgct gggatctgac gctctggacg atttcgatct cgacatgttg
     4681 ggatcagatg cactggatga ctttgacctg gacatgctcg gatcaggatc cgggagtggc
     4741 gaaggcaggg gctccctttt gacgtgcggg gatgttgaag agaaccctgg gcccggatct
     4801 ggaatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     4861 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     4921 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     4981 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     5041 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     5101 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     5161 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     5221 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     5281 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     5341 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     5401 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5461 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaatggcgc
     5521 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5581 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5641 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5701 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5761 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5821 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5881 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5941 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6001 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6061 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatg
     6121 ctgcgtctca agcatacacg ataccgtcga cctcgaggga attaattcga gctcggtacc
     6181 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6241 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6301 gtttctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6361 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6421 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6481 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     6541 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     6601 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     6661 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     6721 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     6781 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     6841 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     6901 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     6961 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     7021 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     7081 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7141 ttacagacaa gctgtgacca tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7201 accgaaacgc gcgagatgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7261 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc cgagcggccg
     7321 cacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgacaggt ggcacttttc
     8101 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8161 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8221 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8281 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8341 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8401 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8461 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8521 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8581 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8641 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8701 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8761 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8821 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8881 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtcccgcg
     8941 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9001 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9061 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9121 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat cgcggccgcc
     9181 aattttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9241 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9301 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     9361 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
     9421 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9481 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9541 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9601 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9661 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9721 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
     9781 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     9841 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     9901 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     9961 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
    10021 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
    10081 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
    10141 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
    10201 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
    10261 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
    10321 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
    10381 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
    10441 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
    10501 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
    10561 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
    10621 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
    10681 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
    10741 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
    10801 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
    10861 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
    10921 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
    10981 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
    11041 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
    11101 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
    11161 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
    11221 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
    11281 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
    11341 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
    11401 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    11461 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    11521 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    11581 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    11641 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    11701 ctattaccac tgccaattac ctgtgg
//
