LOCUS       sequence-383901-        5368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Bcl2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Bray ER, Yungher BJ, Levay K, Ribeiro M, Dvoryanchikov G, Ayupe AC, 
            Thakor K, Marks V, Randolph M, Danzi MC, Schmidt TM, Chaudhari N, 
            Lemmon VP, Hattar S, Park KK
  TITLE     Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion 
            Cells.
  JOURNAL   Neuron. 2019 Aug 21;103(4):642-657.e7. doi: 
            10.1016/j.neuron.2019.05.044. Epub 2019 Jun 26.
  PUBMED    31255486
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.05.044"; journalName: "Neuron"; date: 
            "2019-08-21- 21"; volume: "103"; issue: "4"; pages: "642-657.e7"
COMMENT     Sequence Label: pAAV-Bcl2
FEATURES             Location/Qualifiers
     source          1..5368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     enhancer        217..520
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        521..724
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     675..695
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     721..745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          873..1251
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             2078..2104
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2115..2591
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2314..2333)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2631..2771
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2631..2760
                     /label=AAV2 ITR
     rep_origin      2846..3301
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2933..2952)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3143..3164
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3318..3337)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3437..3459
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3497..3515)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3583..3687
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3688..3756,3757..4548)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3906..3925)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4719..5307
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5208..5227
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtctagt tattaatagt aatcaattac
      181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      361 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      421 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      481 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      541 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      601 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      661 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      721 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      781 tagaagacac cgggaccgat ccagcctccg cggattcgaa tcccggccgg gaacggtgca
      841 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc
      901 ccacaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt
      961 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt
     1021 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat
     1081 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa
     1141 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca
     1201 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc
     1261 aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag aattgggatt cgaacatcga
     1321 ttgaattccc cggggatcct ctagagtcga cctgcagaag ctgatggcgc aagccgggag
     1381 aacagggtat gataaccggg agatcgtgat gaagtacata cattataagc tgtcacagag
     1441 gggctacgag tgggatgctg gagatgcgga cgcggcgccc ctgggggctg cccccacccc
     1501 tggcatcttc tccttccagc ctgagagcaa cccaatgccc gctgtgcacc gggacatggc
     1561 tgccaggacg tctcctctca ggcccctcgt tgccaccgct gggcctgcgc tcagccctgt
     1621 gccacctgtg gtccatctga ccctccgccg ggctggggat gacttctctc gtcgctaccg
     1681 tcgtgacttc gcagagatgt ccagtcagct gcacctgacg cccttcaccg cgaggggacg
     1741 ctttgccacg gtggtggagg aactcttcag ggatggggtg aactggggga ggattgtggc
     1801 cttctttgag ttcggtgggg tcatgtgtgt ggagagcgtc aacagggaga tgtcacccct
     1861 ggtggacaac atcgccctgt ggatgactga gtacctgaac cggcatctgc acacctggat
     1921 ccaggataac ggaggctggg atgcctttgt ggaactatat ggccccagca tgcgacctct
     1981 gtttgatttc tcctggctgt ctctgaagac cctgctcagc ctggccctgg tcggggcctg
     2041 catcactctg ggtgcatacc tgggccacaa ggcagcttac ccatacgatg ttccagatta
     2101 cgcttaggat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     2161 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     2221 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     2281 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     2341 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     2401 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     2461 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     2521 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     2581 tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct
     2641 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     2701 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     2761 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     2821 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2881 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     2941 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3001 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3061 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3121 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     3181 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     3241 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     3301 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3361 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3421 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3481 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3541 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3601 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3661 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3721 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3781 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3841 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3901 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3961 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4021 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4081 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4141 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4201 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4261 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4321 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4381 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4441 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4501 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4561 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4621 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4681 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4741 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4801 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4861 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4921 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4981 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5041 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5101 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5161 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5221 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5281 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5341 gccttttgct ggccttttgc tcacatgt
//
