LOCUS       sequence-383907-       14465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector expressing Jason McClellan lab's 
            6-proline Spike extracellular domain on the cell surface attached to
            PDGFR's transmembrane domain and mCherry transduction marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 14465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)
FEATURES             Location/Qualifiers
     source          1..14465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             99..732
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    779..904
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1401..1634
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1819..1863
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2148..2265
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2326..3504
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2557..3495
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3452..3472
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3574..3603
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7327..7473
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             7489..7542
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7552..8262
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7697..7715)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7965..7984
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8277..8865
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8330..8350)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8748..8759)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8887..8903)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8888..8904)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8994..9227
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9252..9270)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9253..9270)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9560..9579)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9793..9812)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9912..9934
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9972..9990)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(10169..10757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10249..10268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10860..10964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10965..11033,11034..11825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11183..11202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     12067..12084
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12154..12174)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12159..12488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12339..12474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13622..13687
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13817..13837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14262..14396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14299..14318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14353..14372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
       61 aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca
      121 aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
      181 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
      241 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
      301 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
      361 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
      421 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
      481 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
      541 ttgcctgtac tgggactctc tggttagacc agatctgagc ctgggagctc tctggctaac
      601 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
      661 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
      721 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
      781 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
      841 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
      901 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
      961 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     1021 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     1081 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     1141 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     1201 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc
     1261 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     1321 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     1381 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     1441 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     1501 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     1561 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     1621 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     1681 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     1741 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     1801 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     1861 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     1921 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     1981 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     2041 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     2101 gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa
     2161 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2221 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     2281 gacagcagag atccagtttg gccctctgat gagacgaacg cgtgaggctc cggtgcccgt
     2341 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2401 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2461 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2521 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     2581 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     2641 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     2701 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     2761 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     2821 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     2881 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     2941 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3001 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     3061 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     3121 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3181 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3241 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3301 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3361 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     3421 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     3481 tttcttccat ttcaggtgtc gtgagccact atggccttgc ccgttacggc acttctgctc
     3541 ccactcgcat tgctgcttca cgccgcccga ccagaacaga aactcataag tgaagaagac
     3601 cttgggggag gcggttccgg gggaggcggt tctagccagt gcgtgaacct gaccacccga
     3661 acccagctcc caccagccta caccaacagc tttacacggg gcgtgtacta ccctgacaag
     3721 gtgttcagat ctagcgtcct gcacagcact caggacctct tcctgccgtt cttcagcaac
     3781 gtgacatggt tccacgccat ccacgtgagc ggcacaaacg gaaccaagcg gtttgataac
     3841 cccgtcctgc cattcaatga tggagtttac ttcgccagta ccgagaagag taacatcatc
     3901 cggggctgga tcttcggcac caccctggat agcaaaacac agagcctcct gatcgtgaac
     3961 aatgccacga acgtcgtgat caaggtgtgc gagttccagt tttgcaatga tcctttcctg
     4021 ggtgtgtact accacaagaa caacaagagc tggatggaaa gcgagttcag agtctacagc
     4081 agcgccaaca actgcacatt cgagtatgtc tctcagcctt ttctgatgga ccttgagggg
     4141 aaacaaggca acttcaagaa cctgagagaa ttcgtgttca agaacatcga cggctacttc
     4201 aaaatctact ccaagcacac acccatcaac ctggtccggg acctccctca gggcttcagc
     4261 gccctggaac ccctggtcga cctgcccata ggcatcaaca taacgcggtt ccaaaccctg
     4321 ctggccctgc atagatccta cctgactcct ggcgacagca gcagcggatg gaccgccgga
     4381 gctgcagcct actatgtggg ctacctgcaa cctagaacct tcctgctgaa gtacaacgag
     4441 aacggcacaa tcacagacgc cgtcgactgc gccctggacc ctctctctga gacaaagtgc
     4501 accctgaagt ccttcaccgt ggaaaagggc atctaccaga ccagcaactt ccgggtgcag
     4561 cctacagaga gcatcgtgcg atttccaaac attaccaacc tctgcccctt cggcgaggtg
     4621 tttaacgcca caagatttgc ctccgtttac gcctggaata gaaagagaat cagcaattgt
     4681 gtggccgact actccgtgct gtataacagc gcctctttca gcaccttcaa gtgctacggc
     4741 gtttccccaa caaagctgaa tgacctgtgc ttcaccaacg tgtacgccga ctccttcgta
     4801 attagaggcg atgaggtgcg gcagatcgca ccaggccaga ccggtaagat cgctgactac
     4861 aactataagc tgcctgatga ttttacaggc tgcgtgatcg cctggaactc taacaacctg
     4921 gatagcaagg tgggcggcaa ctacaactac ctgtaccggc tgtttcgcaa gtctaacctg
     4981 aaacctttcg agagagacat ctccacagag atctaccagg ccggttctac accttgtaac
     5041 ggggtggaag gcttcaactg ttacttccct ctgcaaagct acggcttcca gcctaccaat
     5101 ggagtcggct accagccata ccgggtggtc gtgctgtcct tcgagttact ccacgccccc
     5161 gccaccgtct gcggtcctaa gaagtccacc aatctggtta agaacaaatg cgtgaacttc
     5221 aacttcaacg gcctgaccgg gaccggcgtg ctgaccgaaa gcaacaaaaa gttcctcccc
     5281 ttccagcagt tcggccgtga tatcgctgac accacagatg ccgtcagaga tccacagacc
     5341 ctggaaatcc tggatattac accctgctcc ttcggaggag tttctgtgat cacccccggg
     5401 accaatacca gcaaccaggt ggctgtgctg taccaagatg ttaactgcac cgaggttcct
     5461 gtggccatcc acgccgatca gctgacacct acttggagag tgtactccac tggctccaat
     5521 gtgttccaga ccagggccgg atgtctgatc ggcgccgagc acgtgaataa cagttacgag
     5581 tgcgacatcc ctatcggcgc cggcatctgt gccagctacc agacccagac aaacagccct
     5641 gggtctgctt cctctgtagc tagccagagc atcatcgcct acaccatgag cctgggcgca
     5701 gagaacagcg tggcctattc caacaactct atcgccattc ccaccaactt tacaattagc
     5761 gtcacaacag agatcctgcc cgtgagcatg accaagacca gcgtggactg tacaatgtac
     5821 atctgtggcg acagcactga atgcagcaac ctgctgctgc aatacggctc cttttgcacc
     5881 caactgaacc gggcgctgac cggaatcgcc gtggaacagg acaaaaatac ccaggaggtg
     5941 ttcgcccaag tgaagcagat ctacaagacc ccacctatca aggacttcgg cggctttaac
     6001 tttagccaga ttctccctga tccttctaag cctagcaagc ggagccctat cgaggatctg
     6061 ctgttcaaca aggtcaccct ggccgatgcc ggctttatca aacagtatgg cgattgcctg
     6121 ggcgacatag ccgccagaga tctgatctgc gcccagaaat tcaacggcct gacagttctc
     6181 ccacctctgc tgaccgacga gatgatcgct cagtacacct ctgccctgct ggctggcacc
     6241 atcacatctg ggtggacatt tggcgccggc cccgccctgc agatcccctt tcccatgcag
     6301 atggcctata gattcaacgg aatcggcgtg acccagaacg tgctgtatga aaaccagaag
     6361 ctgatcgcta accagttcaa ttctgccatc ggcaagatcc aggactccct ctcctctacc
     6421 cccagcgccc tgggcaaact gcaggacgtg gtgaatcaga acgcccaagc cctgaacacc
     6481 ctggtgaagc agctcagcag caattttggc gccatcagct ctgtgctgaa cgatatcctg
     6541 tctagactgg accctccaga agccgaagtc cagatcgata gactgatcac aggcagactg
     6601 cagtccctgc aaacctacgt gacccaacag ctgatcaggg ccgctgaaat aagagccagc
     6661 gccaatctcg ccgctaccaa gatgtccgag tgtgtgctgg gacagtctaa acgcgttgac
     6721 ttctgcggca aaggctatca cctgatgagc ttcccccaga gcgcgccgca cggcgtggtg
     6781 ttcctgcatg tgacatacgt gcctgcccaa gagaagaatt tcacaaccgc ccctgccatc
     6841 tgccacgacg gcaaggccca cttcccaaga gagggcgttt tcgtttccaa tggcacacac
     6901 tggttcgtga cacaaagaaa cttctacgaa ccccagatta tcaccaccga caacaccttc
     6961 gtgagtggca attgtgacgt ggtcatcgga atcgtgaaca acacagtgta cgaccctctg
     7021 caacctgagc tggactcttt taaggaagag ctggacaagt actttaaaaa ccacaccagc
     7081 cccgatgtgg acctgggcga catcagtggc attaacgcca gcgtggtgaa catccaaaag
     7141 gaaatcgaca gactgaacga ggtggccaag aacctgaacg agtccctgat cgacctgcag
     7201 gagctcggca aatacgagca gggatccgga tacatccccg aggcccccag agatggccag
     7261 gcctacgtgc ggaaggacgg cgagtgggta ctgctgagca cattcctggg cagatccggt
     7321 tctaatgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc cttgcccttt
     7381 aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat ctcccttatc
     7441 atcctcatca tgctttggca gaagaagcca cgtggatccg ggagtggcga aggcaggggc
     7501 tcccttttga cgtgcgggga tgttgaagag aaccctgggc ccggatctgg aatggtgagc
     7561 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     7621 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     7681 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     7741 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     7801 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     7861 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     7921 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     7981 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     8041 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     8101 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     8161 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     8221 ggccgccact ccaccggcgg catggacgag ctgtacaagt aatggcgctt atcgataatc
     8281 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8341 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8401 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8461 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     8521 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     8581 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8641 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     8701 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8761 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8821 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatgct gcgtctcaag
     8881 catacacgat accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat
     8941 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     9001 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ttctctggtt
     9061 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9121 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9181 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta
     9241 attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
     9301 taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta
     9361 ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga
     9421 accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc
     9481 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     9541 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     9601 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     9661 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     9721 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     9781 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     9841 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     9901 tgtgaccatc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     9961 gagatgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
    10021 ttcttagacg tcaggtggca cttttcgggg aaatgtgccg agcggccgca cggttatcca
    10081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    10141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    10201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    10261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10501 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10561 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    10621 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10681 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10741 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10801 acgaaaactc acgttaaggg attttggtca tgacaggtgg cacttttcgg ggaaatgtgc
    10861 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10921 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10981 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    11041 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    11101 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    11161 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11221 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    11281 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    11341 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11401 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11461 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11521 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11581 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11641 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtcccgcggt atcattgcag
    11701 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11761 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11821 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11881 aatttaaaag gatctaggtg aagatccttt ttgataatcg cggccgccaa ttttttacgg
    11941 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    12001 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    12061 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    12121 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
    12181 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    12241 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    12301 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    12361 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    12421 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    12481 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
    12541 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
    12601 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
    12661 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
    12721 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
    12781 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
    12841 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
    12901 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
    12961 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
    13021 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
    13081 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
    13141 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
    13201 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
    13261 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
    13321 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
    13381 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
    13441 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
    13501 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
    13561 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
    13621 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
    13681 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
    13741 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
    13801 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
    13861 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
    13921 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
    13981 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
    14041 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
    14101 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
    14161 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    14221 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    14281 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    14341 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    14401 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    14461 ccaat
//
