LOCUS       sequence-383908-        7002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses STAT3 with mitochondrial targeting sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Luo X, Ribeiro M, Bray ER, Lee DH, Yungher BJ, Mehta ST, Thakor KA, 
            Diaz F, Lee JK, Moraes CT, Bixby JL, Lemmon VP, Park KK
  TITLE     Enhanced Transcriptional Activity and Mitochondrial Localization of 
            STAT3 Co-induce Axon Regrowth in the Adult Central Nervous System.
  JOURNAL   Cell Rep. 2016 Apr 12;15(2):398-410. doi: 
            10.1016/j.celrep.2016.03.029. Epub 2016 Mar 31.
  PUBMED    27050520
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.029"; journalName: "Cell Rep"; date: 
            "2016-04-12- 12"; volume: "15"; issue: "2"; pages: "398-410"
COMMENT     Sequence Label: pAAV-STAT3mts
FEATURES             Location/Qualifiers
     source          1..7002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      774..1362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1263..1282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1424..1553
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        1629..1932
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1933..2136
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2087..2107
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2133..2157
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2285..2663
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             2746..2820
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     polyA_signal    5172..5648
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5371..5390)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5688..5828
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5688..5817
                     /label=AAV2 ITR
     rep_origin      5903..6358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5990..6009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6200..6221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6375..6394)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6494..6516
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6554..6572)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6640..6744
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6745..6813,6814..7002,1..603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6963..6982)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
       61 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      121 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      181 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      241 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      301 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      361 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      421 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      481 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      541 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      601 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      661 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      721 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      781 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      841 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      901 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      961 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1021 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1081 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1141 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1201 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1261 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1321 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1381 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc
     1441 gctcgctcgc tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt
     1501 gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg
     1561 cacgcgtcta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     1621 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     1681 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     1741 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     1801 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     1861 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     1921 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     1981 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2041 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2101 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2161 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2221 cgcggattcg aatcccggcc gggaacggtg cattggaacg cggattcccc gtgccaagag
     2281 tgacgtaagt accgcctata gagtctatag gcccacaaaa aatgctttct tcttttaata
     2341 tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc agggcaataa
     2401 tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat aatttctggg
     2461 ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg taactgatgt
     2521 aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct tttattttat
     2581 ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta atcatgttca
     2641 tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc
     2701 actttggcaa agaattggga ttcgaacatc gattgaattg ccaccatgtc cgtcctgacg
     2761 ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag
     2821 atccattcgt tgcgaattcc caccatggct cagtggaacc agctgcagca gctggacaca
     2881 cgctacctgg agcagctgca ccagctgtac agcgacagct tccccatgga gctgcggcag
     2941 ttcctggcac cttggattga gagtcaagac tgggcatatg cagccagcaa agagtcacat
     3001 gccacgttgg tgtttcataa tctcttgggt gaaattgacc agcaatatag ccgattcctg
     3061 caagagtcca atgtcctcta tcagcacaac cttcgaagaa tcaagcagtt tctgcagagc
     3121 aggtatcttg agaagccaat ggaaattgcc cggatcgtgg cccgatgcct gtgggaagag
     3181 tctcgcctcc tccagacggc agccacggca gcccagcaag ggggccaggc caaccaccca
     3241 acagccgccg tagtgacaga gaagcagcag atgttggagc agcatcttca ggatgtccgg
     3301 aagcgagtgc aggatctaga acagaaaatg aaggtggtgg agaacctcca ggacgacttt
     3361 gatttcaact acaaaaccct caagagccaa ggagacatgc aggatctgaa tggaaacaac
     3421 cagtctgtga ccagacagaa gatgcagcag ctggaacaga tgctcacagc cctggaccag
     3481 atgcggagaa gcattgtgag tgagctggcg gggctcttgt cagcaatgga gtacgtgcag
     3541 aagacactga ctgatgaaga gctggctgac tggaagaggc ggcagcagat cgcgtgcatc
     3601 ggaggccctc ccaacatctg cctggaccgt ctggaaaact ggataacttc attagcagaa
     3661 tctcaacttc agacccgcca acaaattaag aaactggagg agctgcagca gaaagtgtcc
     3721 tacaagggcg accctatcgt gcagcaccgg cccatgctgg aggagaggat cgtggagctg
     3781 ttcagaaact taatgaagag tgccttcgtg gtggagcggc agccctgcat gcccatgcac
     3841 ccggaccggc ccttagtcat caagactggt gtccagttta ccacgaaagt caggttgctg
     3901 gtcaaatttc ctgagttgaa ttatcagctt aaaattaaag tgtgcattga taaagactct
     3961 ggggatgttg ctgccctcag agggtctcgg aaatttaaca ttctgggcac gaacacaaaa
     4021 gtgatgaaca tggaggagtc taacaacggc agcctgtctg cagagttcaa gcacctgacc
     4081 cttagggagc agagatgtgg gaatggaggc cgtgccaatt gtgatgcctc cttgatcgtg
     4141 actgcggcgc tgcacctgat caccttcgag actgaggtgt accaccaagg cctcaagatt
     4201 gacctagaga cccactcctt gccagctgcg gcgatctcca acatctgtca gatgccaaat
     4261 gcttgggcat caatcctgtg gtataacatg ctgaccaata accccaagaa cgtgaacttc
     4321 ttcactaagc cgccaattgg aacctgggac caagtggccg aggtgctcag ctggcagttc
     4381 tcgtccacca ccaagcgggg gctgagcatc gagcagctga caacgctggc tgagaagctc
     4441 ctagggcctg gtgtgaacta ctcagggtgt cagatcacat gggctaaatt ctgcaaagaa
     4501 aacatggctg gcaagggcct ctccttctgg gtctggctag acaatatcat cgaccttgtg
     4561 aaaaagtata tcttggccct ttggaatgaa gggtacatca tgggtttcat cagcagggag
     4621 cgggagcggg ccatcctaag cacaaagccc ccgggcacct tcctactgcg cttcagcgag
     4681 agcagcaaag aaggaggggt cactttcact tgggtggaaa aggacatcag tggcaagacc
     4741 cagatccagt ctgtagagcc atacaccaag cagcagctga acaacatgtc atttgctgaa
     4801 atcatcatgg gctataagat catggatgcg accaacatcc tggtgtctcc acttgtctac
     4861 ctctaccccg acattcccaa ggaggaggca tttggaaagt actgtaggcc cgagagccag
     4921 gaacaccccg aagccgaccc aggtagtgct gccccgtacc tgaagaccaa gttcatctgt
     4981 gtgacaccaa cgacctgcag caataccatt gacctgccga tgtccccccg cactttagat
     5041 tcattgatgc agtttggaaa taacggtgaa ggtgctgagc cctcagcagg agggcagttt
     5101 gagtcgctca cgtttgacat ggatctgacc tcggagtgtg ctacctcccc catgtagctc
     5161 gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
     5221 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
     5281 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag
     5341 ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg
     5401 cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
     5461 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt
     5521 ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
     5581 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
     5641 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt
     5701 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     5761 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     5821 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5881 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5941 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
     6001 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6061 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     6121 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6181 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
     6241 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
     6301 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     6361 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     6421 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6481 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6541 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6601 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6661 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6721 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6781 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6841 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6901 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6961 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aa
//
