LOCUS       sequence-383909-       10642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transducition vector for a GAL4-responsive tagBFP 
            reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 10642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)
FEATURES             Location/Qualifiers
     source          1..10642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             85..718
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    765..890
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1387..1620
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1805..1849
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2134..2251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2514..3215
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        3290..3789
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3325..3348)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3722..3741
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(3834..3836,3837..3839,3840..4553)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3879..3900)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4140..4159)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4487..4508
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4590..5178
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4643..4663)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5061..5072)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5181..5197)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5182..5198)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5288..5521
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5546..5564)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5547..5564)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5854..5873)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6087..6106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6206..6228
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6266..6284)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6352..6456
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6457..6525,6526..7317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6675..6694)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7488..8076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7977..7996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8230..8247
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8317..8337)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8322..8651
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8502..8637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9785..9850
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9980..10000
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10425..10559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10462..10481)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10516..10535
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
       61 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
      121 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
      181 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
      241 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
      301 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
      361 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
      421 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
      481 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
      541 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      601 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      661 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      721 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      781 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      841 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      901 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      961 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1021 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1081 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1141 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1201 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1261 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1321 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1381 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1441 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1501 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1561 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1621 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1681 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     1741 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     1801 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     1861 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     1921 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     1981 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2041 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     2101 acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2161 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2221 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2281 agtttggatc gataagcttg atatcgaatt cggagcactg tcctccgaac gtcggagcac
     2341 tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct ccgaacggag
     2401 catgtcctcc gaacgtcgga gcactgtcct ccgaacgact agtctagagg gtatataatg
     2461 ggggccacta gtctactacc agagctcatc gctagcgcta ccggatcgcc accatgagcg
     2521 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     2581 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     2641 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     2701 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     2761 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     2821 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     2881 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     2941 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     3001 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     3061 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     3121 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     3181 acctccctag caaactgggg cacaagctta attaagatcc ttgacttgcg gccgcaactc
     3241 ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgaccg ggtaggggag
     3301 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     3361 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     3421 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     3481 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     3541 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     3601 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     3661 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     3721 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     3781 ggcctttcga attctcacgc gtcaagtgga gcaaggcagg tggacagtgg atcatggtga
     3841 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3901 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3961 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4021 ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg
     4081 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4141 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4201 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4261 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4321 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4381 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4441 gctaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4501 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagatccgg
     4561 catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     4621 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4681 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4741 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4801 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4861 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4921 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4981 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5041 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5101 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5161 tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta
     5221 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5281 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     5341 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5401 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5461 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5521 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     5581 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     5641 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     5701 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     5761 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     5821 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     5881 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     5941 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     6001 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     6061 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     6121 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6181 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6241 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     6301 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     6361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     6421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     6481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     6541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     6601 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     6661 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     6721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6961 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7021 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7081 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7141 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7201 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7261 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7321 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7501 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7561 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7621 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7681 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7741 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7801 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7861 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7921 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7981 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8041 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8101 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8161 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8221 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8281 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
     8341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8401 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8461 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8521 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8581 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8641 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     8701 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     8761 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     8821 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     8881 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     8941 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     9001 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     9061 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     9121 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     9181 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     9241 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     9301 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     9361 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     9421 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     9481 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     9541 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     9601 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     9661 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
     9721 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     9781 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     9841 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     9901 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     9961 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
    10021 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
    10081 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
    10141 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
    10201 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
    10261 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
    10321 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
    10381 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
    10441 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10501 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    10561 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
    10621 ctgccaatta cctgtggttt ca
//
