LOCUS       sequence-383910-       10973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector expressing LCB3 on the cell surface 
            attached to PDGFR's transmembrane domain and mCherry transduction 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 10973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)
FEATURES             Location/Qualifiers
     source          1..10973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             81..714
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    761..886
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1383..1616
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1801..1845
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2130..2247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2308..3486
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2539..3477
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3434..3454
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3556..3585
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3817..3963
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             3979..4032
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4042..4752
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4187..4205)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4455..4474
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4767..5355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4820..4840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5238..5249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5377..5393)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5378..5394)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5484..5717
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5742..5760)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5743..5760)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6050..6069)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6283..6302)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6402..6424
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6462..6480)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(6659..7247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6739..6758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7350..7454
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7455..7523,7524..8315)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7673..7692)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     8557..8574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8644..8664)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8649..8978
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8829..8964
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10112..10177
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10307..10327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10752..10886
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10789..10808)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10843..10862
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
       61 tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct
      121 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
      181 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
      241 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
      301 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
      361 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
      421 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
      481 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggactc
      541 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      601 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      661 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      721 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      781 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      841 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      901 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
      961 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1021 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1081 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1141 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1201 aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat
     1261 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1321 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1381 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1441 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1501 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1561 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1621 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1681 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1741 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1801 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1861 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1921 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1981 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2041 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     2101 tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2161 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2221 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2281 tggccctctg atgagacgaa cgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca
     2341 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     2401 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     2461 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     2521 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     2581 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     2641 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     2701 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     2761 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     2821 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     2881 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     2941 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     3001 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     3061 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     3121 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     3181 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     3241 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     3301 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     3361 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     3421 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     3481 tcgtgagcca ctatggcctt gcccgttacg gcacttctgc tcccactcgc attgctgctt
     3541 cacgccgccc gaccagaaca gaaactcata agtgaagaag accttggggg aggcggttcc
     3601 gggggaggcg gttctaacga tgacgagctc catatgcaga tgaccgatct ggtgtacgag
     3661 gcactacact ttgctaaaga cgaggaaatc cagaagcacg tcttccagct gttcgagaag
     3721 gccacaaagg cctataaaaa taaagacagg caaaaactgg aaaaggttgt ggaggaactg
     3781 aaggaattgt tagagagact tctcagcggt tctaatgctg tgggccagga cacgcaggag
     3841 gtcatcgtgg tgccacactc cttgcccttt aaggtggtgg tgatctcagc catcctggcc
     3901 ctggtggtgc tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca
     3961 cgtggatccg ggagtggcga aggcaggggc tcccttttga cgtgcgggga tgttgaagag
     4021 aaccctgggc ccggatctgg aatggtgagc aagggcgagg aggataacat ggccatcatc
     4081 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4141 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4201 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4261 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4321 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4381 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4441 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4501 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4561 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4621 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4681 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4741 ctgtacaagt aatggcgctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     4801 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4861 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4921 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4981 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5041 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5101 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5161 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5221 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5281 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5341 gggccgcctc cccgcatgct gcgtctcaag catacacgat accgtcgacc tcgagggaat
     5401 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5461 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5521 gctttttgct tgtactgggt ttctctggtt agaccagatc tgagcctggg agctctctgg
     5581 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5641 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5701 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     5761 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     5821 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     5881 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     5941 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     6001 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     6061 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     6121 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     6181 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     6241 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     6301 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     6361 tctgctcccg gcatccgctt acagacaagc tgtgaccatc tccgggagct gcatgtgtca
     6421 gaggttttca ccgtcatcac cgaaacgcgc gagatgaaag ggcctcgtga tacgcctatt
     6481 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6541 aaatgtgccg agcggccgca cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6601 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6661 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6721 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6781 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6841 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6901 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6961 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7021 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7081 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7141 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7201 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7261 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7321 tgacaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7381 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7441 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7501 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7561 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7621 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7681 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7741 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7801 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7861 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7921 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7981 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8041 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8101 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8161 gtgagcgtgg gtcccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8221 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8281 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8341 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8401 ttgataatcg cggccgccaa ttttttacgg ttcctggcct tttgctggcc ttttgctcac
     8461 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8521 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8581 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8641 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     8701 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     8761 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     8821 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8881 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8941 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     9001 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     9061 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     9121 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     9181 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     9241 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     9301 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     9361 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     9421 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     9481 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     9541 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     9601 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     9661 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     9721 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     9781 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     9841 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     9901 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     9961 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
    10021 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
    10081 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
    10141 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
    10201 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
    10261 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
    10321 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
    10381 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
    10441 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
    10501 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
    10561 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
    10621 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
    10681 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
    10741 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10801 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10861 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
    10921 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat tta
//
