LOCUS       sequence-383911-       10949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector expressing LCB1 on the cell surface 
            attached to PDGFR's transmembrane domain and mCherry transduction 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 10949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)
FEATURES             Location/Qualifiers
     source          1..10949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             84..717
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    764..889
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1386..1619
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1804..1848
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2133..2250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2311..3489
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2542..3480
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3437..3457
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3559..3588
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3796..3942
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             3958..4011
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4021..4731
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4166..4184)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4434..4453
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4746..5334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4799..4819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5217..5228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5356..5372)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5357..5373)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5463..5696
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5721..5739)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5722..5739)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6029..6048)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6262..6281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6381..6403
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6441..6459)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(6638..7226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6718..6737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7329..7433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7434..7502,7503..8294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7652..7671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     8536..8553
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8623..8643)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8628..8957
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8808..8943
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10091..10156
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10286..10306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10731..10865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10768..10787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10822..10841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
       61 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
      121 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
      181 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
      241 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
      301 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
      361 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
      421 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
      481 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactggga
      541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
      601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
      661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
      721 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
      781 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
      841 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
      901 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
      961 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1021 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1081 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1141 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1201 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     1261 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1321 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1381 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1441 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1501 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1561 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1621 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1681 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1741 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1801 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1861 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1921 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1981 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2041 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     2101 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2161 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2221 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2281 gtttggccct ctgatgagac gaacgcgtga ggctccggtg cccgtcagtg ggcagagcgc
     2341 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     2401 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     2461 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     2521 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     2581 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     2641 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     2701 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     2761 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     2821 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     2881 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     2941 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     3001 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     3061 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     3121 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     3181 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     3241 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     3301 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     3361 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     3421 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     3481 gtgtcgtgag ccactatggc cttgcccgtt acggcacttc tgctcccact cgcattgctg
     3541 cttcacgccg cccgaccaga acagaaactc ataagtgaag aagaccttgg gggaggcggt
     3601 tccgggggag gcggttctga caaggaaaac attctccaga aaatatacga gatcatgaaa
     3661 accctcgagc aactgggcca cgcagaggct agcatgcagg tgtccgatct gatctatgaa
     3721 ttcatgaagc agggggacga gagactgttg gaggaagccg agcggttact tgaagaggtc
     3781 gaaaggggtt ctaatgctgt gggccaggac acgcaggagg tcatcgtggt gccacactcc
     3841 ttgcccttta aggtggtggt gatctcagcc atcctggccc tggtggtgct caccatcatc
     3901 tcccttatca tcctcatcat gctttggcag aagaagccac gtggatccgg gagtggcgaa
     3961 ggcaggggct cccttttgac gtgcggggat gttgaagaga accctgggcc cggatctgga
     4021 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     4081 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     4141 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     4201 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     4261 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     4321 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     4381 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     4441 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     4501 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     4561 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     4621 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     4681 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta atggcgctta
     4741 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4801 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4861 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4921 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4981 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5041 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5101 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5161 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5221 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5281 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatgctg
     5341 cgtctcaagc atacacgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     5401 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5461 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtt
     5521 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5581 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5641 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     5701 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     5761 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     5821 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     5881 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     5941 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     6001 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     6061 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     6121 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     6181 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     6241 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     6301 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     6361 cagacaagct gtgaccatct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     6421 gaaacgcgcg agatgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     6481 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgccga gcggccgcac
     6541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gacaggtggc acttttcggg
     7321 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     7381 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     7441 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     7501 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     7561 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     7621 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7681 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7741 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7801 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7861 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7921 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7981 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8041 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8101 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tcccgcggta
     8161 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8221 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8281 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8341 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatcgc ggccgccaat
     8401 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8461 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8521 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8581 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
     8641 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8701 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8761 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8821 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8881 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8941 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
     9001 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     9061 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     9121 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     9181 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     9241 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
     9301 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     9361 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     9421 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     9481 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     9541 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     9601 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     9661 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     9721 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     9781 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     9841 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     9901 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     9961 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
    10021 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
    10081 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
    10141 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
    10201 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
    10261 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
    10321 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
    10381 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
    10441 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
    10501 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
    10561 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
    10621 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
    10681 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    10741 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10801 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10861 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    10921 ttaccactgc caattacctg tggtttcat
//
