LOCUS       sequence-383916-       12538 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector expressing ACE2-ECD SynNotch and 
            mCherry transduction marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Weinberg ZY, Hilburger CE, Kim M, Cao L, Khalid M, Elmes S, Diwanji 
            D, Hernandez E, Lopez J, Schaefer K, Smith AM, Zhou F, QCRG 
            Structural Biology Consortium, Kumar GR, Ott M, Baker D, El-Samad H
  TITLE     Sentinel cells enable genetic detection of SARS-CoV-2 Spike protein
  JOURNAL   bioRxiv 2021.04.20.440678
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.20.440678"
COMMENT     Sequence Label: PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)
FEATURES             Location/Qualifiers
     source          1..12538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             103..736
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    783..908
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1405..1638
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1823..1867
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2152..2269
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2325..2824
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2360..2383)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2757..2776
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2894..2923
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5702..6142
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             6179..6328
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    6354..6942
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6407..6427)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6825..6836)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6964..6980)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6965..6981)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7071..7304
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7329..7347)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7330..7347)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7637..7656)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7870..7889)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7989..8011
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8049..8067)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(8246..8834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8326..8345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8937..9041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9042..9110,9111..9902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9260..9279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     10144..10161
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10231..10251)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10236..10565
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10416..10551
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11699..11764
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11894..11914
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12339..12473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12376..12395)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12430..12449
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
       61 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
      121 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
      181 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
      241 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
      301 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
      361 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
      421 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
      481 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
      541 ctttttgcct gtactgggac tctctggtta gaccagatct gagcctggga gctctctggc
      601 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      661 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      721 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
      781 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
      841 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
      901 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
      961 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     1021 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     1081 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     1141 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     1201 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     1261 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1321 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1381 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1441 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1501 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1561 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1621 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1681 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1741 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1801 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1861 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     1921 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     1981 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     2041 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     2101 attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag
     2161 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2221 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     2281 cagggacagc agagatccag tttggccctc tgatgagacg aacggggtag gggaggcgct
     2341 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     2401 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     2461 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     2521 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcatgtctca ctagtctcgt
     2581 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     2641 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     2701 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     2761 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     2821 ttcggccact atggccttgc ccgttacggc acttctgctc ccactcgcat tgctgcttca
     2881 cgccgcccga ccagaacaga aactcataag tgaagaagac cttcagtcca ccattgagga
     2941 acaggccaag acatttttgg acaagtttaa ccacgaagcc gaagacctgt tctatcaaag
     3001 ttcacttgct tcttggaatt ataacaccaa tattactgaa gagaatgtcc aaaacatgaa
     3061 taatgctggg gacaaatggt ctgccttttt aaaggaacag tccacacttg cccaaatgta
     3121 tccactacaa gaaattcaga atctcacagt caagcttcag ctgcaagctc ttcagcaaaa
     3181 tgggtcttca gtgctctcag aagacaagag caaacggttg aacacaattc taaatacaat
     3241 gagcaccatc tacagtactg gaaaagtttg taacccagat aatccacaag aatgcttatt
     3301 acttgaacca ggtttgaatg aaataatggc aaacagttta gactacaatg agaggctctg
     3361 ggcttgggaa agctggaggt ctgaggtcgg caagcagctg aggccattat atgaagagta
     3421 tgtggtcttg aaaaatgaga tggcaagagc aaatcattat gaggactatg gggattattg
     3481 gagaggagac tatgaagtaa atggggtaga tggctatgac tacagccgcg gccagttgat
     3541 tgaagatgtg gaacatacct ttgaagagat taaaccatta tatgaacatc ttcatgccta
     3601 tgtgagggca aagttgatga atgcctatcc ttcctatatc agtccaattg gatgcctccc
     3661 tgctcatttg cttggtgata tgtggggtag attttggaca aatctgtact ctttgacagt
     3721 tccctttgga cagaaaccaa acatagatgt tactgatgca atggtggacc aggcctggga
     3781 tgcacagaga atattcaagg aggccgagaa gttctttgta tctgttggtc ttcctaatat
     3841 gactcaagga ttctgggaaa attccatgct aacggaccca ggaaatgttc agaaagcagt
     3901 ctgccatccc acagcttggg acctggggaa gggcgacttc agaatcctta tgtgcacaaa
     3961 ggtgacaatg gacgacttcc tgacagctca tcatgagatg gggcatatcc agtatgatat
     4021 ggcatatgct gcacaacctt ttctgctaag aaatggagct aatgaaggat tccatgaagc
     4081 tgttggggaa atcatgtcac tttctgctgc cacacctaag catttaaaat ccattggtct
     4141 tctgtcaccc gattttcaag aagacaatga aacagaaata aacttcctgc tcaaacaagc
     4201 actcacgatt gttgggactc tgccatttac ttacatgtta gagaagtgga ggtggatggt
     4261 ctttaaaggg gaaattccca aagaccagtg gatgaaaaag tggtgggaga tgaagcgaga
     4321 gatagttggg gtggtggaac ctgtgcccca tgatgaaaca tactgtgacc ccgcatctct
     4381 gttccatgtt tctaatgatt actcattcat tcgatattac acaaggaccc tttaccaatt
     4441 ccagtttcaa gaagcacttt gtcaagcagc taaacatgaa ggccctctgc acaaatgtga
     4501 catctcaaac tctacagaag ctggacagaa actgttcaat atgctgaggc ttggaaaatc
     4561 agaaccctgg accctagcat tggaaaatgt tgtaggagca aagaacatga atgtaaggcc
     4621 actgctcaac tactttgagc ccttatttac ctggctgaaa gaccagaaca aaaattcttt
     4681 tgtgggatgg agtaccgact ggagtccata tgcagacggt tctatcctgg actacagctt
     4741 cacaggtggc gctgggcgcg acattccccc accgcagatt gaggaggcct gtgagctgcc
     4801 tgagtgccag gtggatgcag gcaataaggt ctgcaacctg cagtgtaata atcacgcatg
     4861 tggctgggat ggtggcgact gctccctcaa cttcaatgac ccctggaaga actgcacgca
     4921 gtctctacag tgctggaagt attttagcga cggccactgt gacagccagt gcaactcggc
     4981 cggctgcctc tttgatggct tcgactgcca gctcaccgag ggacagtgca accccctgta
     5041 tgaccagtac tgcaaggacc acttcagtga tggccactgc gaccagggct gtaacagtgc
     5101 cgaatgtgag tgggatggcc tagactgtgc tgagcatgta cccgagcggc tggcagccgg
     5161 caccctggtg ctggtggtgc tgcttccacc cgaccagcta cggaacaact ccttccactt
     5221 tctgcgggag ctcagccacg tgctgcacac caacgtggtc ttcaagcgtg atgcgcaagg
     5281 ccagcagatg atcttcccgt actatggcca cgaggaagag ctgcgcaagc acccaatcaa
     5341 gcgctctaca gtgggttggg ccacctcttc actgcttcct ggtaccagtg gtgggcgcca
     5401 gcgcagggag ctggacccca tggacatccg tggctccatt gtctacctgg agatcgacaa
     5461 ccggcaatgt gtgcagtcat cctcgcagtg cttccagagt gccaccgatg tggctgcctt
     5521 cctaggtgct cttgcgtcac ttggcagcct caatattcct tacaagattg aggccgtgaa
     5581 gagtgagccg gtggagcctc cgctgccctc gcagctgcac ctcatgtacg tggcagcggc
     5641 tgccttcgtg ctcctgttct ttgtgggctg tggggtgctg ctgtcccgca agcgccggcg
     5701 gatgaagctg ctgagcagca tcgagcaggc ctgtgacatc tgccggctga agaaactgaa
     5761 gtgcagcaaa gaaaagccca agtgcgccaa gtgcctgaag aacaactggg agtgccggta
     5821 cagccccaag accaagagaa gccccctgac cagagcccac ctgaccgagg tggaaagccg
     5881 gctggaaaga ctggaacagc tgtttctgct gatcttccca cgcgaggacc tggacatgat
     5941 cctgaagatg gacagcctgc aggacatcaa ggccctgctg accggcctgt tcgtgcagga
     6001 caacgtgaac aaggacgccg tgaccgacag actggccagc gtggaaaccg acatgcccct
     6061 gaccctgcgg cagcacagaa tcagcgccac cagcagcagc gaggaaagca gcaacaaggg
     6121 ccagcggcag ctgacagtgt ctgctgctgc aggcggaagc ggaggctctg gcggatctga
     6181 tgccctggac gacttcgacc tggatatgct gggcagcgac gccctggatg attttgatct
     6241 ggacatgctg ggatctgacg ctctggacga tttcgatctc gacatgttgg gatcagatgc
     6301 actggatgac tttgacctgg acatgctcgg atcaggatcc taagcttatc gataatcaac
     6361 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6421 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6481 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6541 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6601 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6721 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6781 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6841 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6901 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatgctgcg tctcaagcat
     6961 acacgatacc gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac
     7021 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7081 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtttc tctggttaga
     7141 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7201 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7261 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     7321 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     7381 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     7441 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     7501 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     7561 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     7621 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     7681 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     7741 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     7801 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     7861 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     7921 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     7981 gaccatctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     8041 atgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     8101 ttagacgtca ggtggcactt ttcggggaaa tgtgccgagc ggccgcacgg ttatccacag
     8161 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8221 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8281 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8341 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8401 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8461 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8521 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8581 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8641 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8701 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8761 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8821 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8881 aaaactcacg ttaagggatt ttggtcatga caggtggcac ttttcgggga aatgtgcgcg
     8941 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9001 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9061 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9121 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9181 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9241 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9301 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9361 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9421 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9481 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9541 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9601 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9661 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9721 ggtttattgc tgataaatct ggagccggtg agcgtgggtc ccgcggtatc attgcagcac
     9781 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9841 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9901 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9961 ttaaaaggat ctaggtgaag atcctttttg ataatcgcgg ccgccaattt tttacggttc
    10021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    10201 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
    10261 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    10321 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10381 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
    10441 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    10501 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    10561 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
    10621 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
    10681 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
    10741 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
    10801 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
    10861 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
    10921 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
    10981 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
    11041 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
    11101 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
    11161 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
    11221 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
    11281 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
    11341 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
    11401 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
    11461 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
    11521 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
    11581 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
    11641 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
    11701 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
    11761 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
    11821 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
    11881 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
    11941 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
    12001 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
    12061 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
    12121 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
    12181 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
    12241 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    12301 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    12361 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    12421 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    12481 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgc
//
