LOCUS       sequence-383921-       15050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Oncogenic with addition of a myristylation signal and expresses in 
            avian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Chang HW, Aoki M, Fruman D, Auger KR, Bellacosa A, Tsichlis PN, 
            Cantley LC, Roberts TM, Vogt PK
  TITLE     Transformation of chicken cells by the gene encoding the catalytic 
            subunit of PI 3-kinase.
  JOURNAL   Science. 1997 Jun 20;276(5320):1848-50. doi: 
            10.1126/science.276.5320.1848.
  PUBMED    9188528
REFERENCE   2  (bases 1 to 15050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.276.5320"; journalName: "Science"; date: 
            "1997-06-20- 20"; volume: "276"; issue: "5320"; pages: "1848-50"
COMMENT     Sequence Label: RCAS myr-c-P3k
FEATURES             Location/Qualifiers
     source          1..15050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7088..7129
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      10267..10276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10331..10354
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        10591..10852
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(11442..11464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11453..11469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11477..11483,11484..11501,11502..11507))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(11550..11568)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11636..11740
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11741..11809,11810..12601)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11959..11978)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12772..13360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13261..13280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13514..13531
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13546..13686
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13612..13631)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13772..13794
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(13788..13979)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        join(14822..15050,1..33)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac cgttgattcc
       61 ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc cgacgtgata
      121 gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc cgtctcgtct
      181 atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga ggcagaagct
      241 gagtaccgtc ggagggagct ccagggcccg gagcgactga cccctgccga gaactcagag
      301 ggtcgtcgga agacggagag tgagcccgac gaccacccca ggcacgtctt tggtcggcct
      361 gcggatcaag catggaagcc gtcattaagg tgatttcgtc cgcgtgtaaa acctattgcg
      421 ggaaaatctc tccttctaag aaggaaatag gggccatgtt gtccctgtta caaaaggaag
      481 ggttgcttat gtctccctca gatttatatt ctccggggtc ctgggatccc atcactgcgg
      541 cgctctccca gcgggcaatg gtacttggaa aatcgggaga gttaaaaacc tggggattgg
      601 ttttgggggc attgaaggcg gctcgagagg aacaggttac atctgagcaa gcaaagtttt
      661 ggttgggatt agggggaggg agggtctctc ccccaggtcc ggagtgcatc gagaaaccag
      721 ctacggagcg gcgaatcgac aaaggggagg aggtgggaga aacaactgtg cagcgagatg
      781 cgaagatggc gccagaggaa gcggccacac ctaaaaccgt tggcacatcc tgctatcatt
      841 gcggaacagc tgttggctgc aattgcgcca ccgccacagc ctcggcccct cctccccctt
      901 atgtggggag tggtttgtat ccttccctgg cgggggtggg agagcagcag ggccagggag
      961 ataacacgtc tcggggggcg gagcagccaa gggaggagcc agggcacgcg ggtcaggccc
     1021 ctgggccggc cctgactgac tgggcaaggg taagggagga gcttgcgagt actggtccgc
     1081 ccgtggtggc catgcctgta gtgattaaga cagagggacc cgcctggacc cctctggagc
     1141 caaaattgat cacaagactg gctgatacgg tcaggaccaa gggcttacga tccccgatca
     1201 ctatggcaga agtggaagcg ctcatgtcct ccccgttgct gccgcatgac gtcacgaatc
     1261 taatgagagt gattttagga cctgccccat atgccttatg gatggacgct tggggagtcc
     1321 aactccagac ggttatagcg gcagccactc gcgacccccg acacccagcg aacggtcaag
     1381 ggcgggggga acggactaac ttggatcgat taaagggctt agctgatggg atggtgggca
     1441 acccacaggg tcaggccgca ttattaagac cgggggaatt ggttgctatt acggcgtcgg
     1501 ctctccaggc gtttagagaa gttgcccggc tggcggaacc tgcaggtcca tgggcggaca
     1561 tcacgcaggg accatctgag tcctttgttg attttgccaa tcggcttata aaggcggttg
     1621 aggggtcaga tctcccgcct tccgcgcggg ctccggtgat cattgactgc tttaggcaga
     1681 agtcacagcc agatattcag cagcttatac gggcagcacc ctccacgctg accaccccag
     1741 gagagataat caaatatgtg ctagacaggc agaagattgc ccctcttacg gatcaaggca
     1801 tagccgcggc catgtcgtct gctatccagc ccttagttat ggcagtagtc aatagagaga
     1861 gggatggaca aactgggtcg ggtggtcgtg cccgagggct ctgctacact tgtggatccc
     1921 cgggacatta tcaggcacag tgcccgaaaa aacgaaagtc aggaaacagc cgtgagcgat
     1981 gtcagctgtg tgacgggatg ggacacaacg ctaaacagtg taggaagcgg gatggcaacc
     2041 agggccaacg cccaggaaga ggtctctctt cggggccgtg gcccggccct gagcagcctg
     2101 ccgtctcgtt agcgatgaca atggaacata aagatcgccc cttggttagg gtcattctga
     2161 ctaacactgg gagtcatcca gtcaaacaac gttcggtgta tatcaccgcg ctgttggact
     2221 ccggagcgga catcactatt atttcggagg aggattggcc tactgattgg ccggtggtgg
     2281 acaccgcgaa cccacagatc catggcatag gagggggaat tcccatgcga aaatcccggg
     2341 atatgataga ggtgggggtt attaaccgag acgggtcgtt ggagcgaccc ctgctcctct
     2401 tccccgcagt cgctatggtt agagggagta tcctaggaag agattgtctg cagggcctag
     2461 ggctccgctt gacaaattta tagggagggc cactgttctt actgttgcgc tacatctggc
     2521 tattccgctc aaatggaagt cagaccgcac gcctgtgtgg attgaccagt ggccccttcc
     2581 tgaaggtaaa cttgtagcgc taacgcaatt agtggaaaaa gaattacagt taggacatat
     2641 agagccctca cttagttgtt ggaacacacc tgtttttgtg atccggaagg cttccgggtc
     2701 ttatcgctta ttgcatgatt tgcgcgctgt taacgccaag cttgttcctt ttggggccgt
     2761 ccaacagggg gcgccagttc tctccgcgct cccgcgtggc tggcccctga tggtcctaga
     2821 cctcaaggat tgcttctttt ctatccctct tgcggaacaa gatcgcgaag cttttgcatt
     2881 tacgctcccc tctgtgaata accaggcccc cgctcgaaga ttccaatgga aggtcttgcc
     2941 ccaagggatg acctgttctc ccactatctg tcagttggta gtgggtcagg tgctcgagcc
     3001 cttgcgactc aagcacccag ctctgcgcat gttgcattat atggacgatc ttttgctagc
     3061 cgcctcaagt catgatgggt tggaagcggc agggaaggag gttatcggta cattggaaag
     3121 agccgggttc actatttcgc cggataagat ccagagggag cccggagtac aatatcttgg
     3181 gtacaagtta ggcagtacgt atgtagcacc cgtaggcttg gtagcagaac ccaggatagc
     3241 caccttgtgg gatgttcaaa agctggtggg gtcacttcag tggcttcgcc cagcgttagg
     3301 gatcccgcca cgactgatgg gtccctttta tgagcagtta cgagggtcag atcctaacga
     3361 ggcgagggaa tggaatctag acatgaaaat ggcctggaga gagatcgtac agcttagcac
     3421 tactgctgcc ttggaacgat gggaccctgc ccagcctctg gaaggagcgg tcgctagatg
     3481 tgaacagggg gcaatagggg tcctgggaca gggactgtcc acacacccaa ggccatgttt
     3541 gtggttattc tccacccaac ccaccaaggc gtttactgct tggttagaag tgctcaccct
     3601 tttgattact aagctacgcg cttcggcagt gcgaaccttt ggcaaggagg ttgatatcct
     3661 cctgttgcct gcatgcttcc gggaggacct tccgctcccg gaggggatcc tgttagcact
     3721 tagggggttt gcaggaaaaa tcaggagtag tgacacgcca tctatttttg acattgcgcg
     3781 tccactgcat gtttctctga aagtgagggt taccgaccac cctgtgccgg gacccactgt
     3841 ctttaccgac gcctcctcaa gcacccataa aggggtggta gtctggaggg agggcccaag
     3901 gtgggagata aaagaaatag ttgatttggg ggcaagtgta caacaactgg aggcacgcgc
     3961 tgtggccatg gcacttctgc tgtggccgac aacgcccact aatgtagtga ctgactctgc
     4021 gtttgttgcg aaaatgttac tcaagatggg acaggaggga gtcccgtcta cagcggcagc
     4081 ttttatttta gaggatgcgt taagccaaag gtcagccatg gccgccgttc tccacgtgcg
     4141 gagtcattct gaagtgccag ggtttttcac agaaggaaat gacgtggcag atagccaagc
     4201 cacctttcaa gcgtatccct tgagagaggc taaagatctt cataccgctc tccatattgg
     4261 accccgcgcg ctatccaaag cgtgtaatat atctatgcag caggctaggg aggttgttca
     4321 gacctgcccg cattgtaatt cagcccctgc gttggaggcc ggggtaaacc ctaggggttt
     4381 gggaccccta cagatatggc agacagactt tacgcttgag cctagaatgg ctccccgttc
     4441 ctggctcgct gttactgtgg acaccgcctc atcagcgata gtcgtaactc agcatggccg
     4501 tgttacatcg gttgctgcac aacatcattg ggccacggct atcgccgttt tgggaagacc
     4561 aaaggccata aaaacagata acgggtcctg cttcacgtct aaatccacgc gggagtggct
     4621 cgcgagatgg gggatagcac acaccaccgg gattccggga aattcccagg gtcaagctat
     4681 ggtagagcgg gccaaccggc tcctgaaaga taagatccgt gtgcttgcgg aaggggacgg
     4741 ctttatgaaa agaatccccg ccagcaaaca gggggaacta ctagccaaag caatgtatgc
     4801 cctcaatcac tttgagcgtg gtgaaaacac aaaaacaccg gtacaaaaac actggagacc
     4861 taccgttctt acagaaggac ccccggttaa aatacgaata gagacagggg agtgggaaaa
     4921 aggatggaac gtgctggtct ggggacgagg ttatgccgct gtgaaaaaca gggacactga
     4981 taaggttatt tgggtaccct ctcggaaagt taaaccggat gtcacccaaa aggatgaggt
     5041 gactaagaaa gatgaggcga gccctctttt tgcaggcatt tctgactgga taccctggga
     5101 agacgagcaa gaaggactcc aaggagaaac cgctagcaac aagcaagaaa gacccggaga
     5161 agacaccctt gctgccaacg agagttaatt atattctcat tattggtgtc ctggtcttgt
     5221 gtgaggttac gggggtaaga gctgatgtcc acttactcga gcagccaggg aacctttgga
     5281 ttacatgggc caaccgtaca ggccaaacgg atttttgcct ctctacacag tcagccacct
     5341 ccccttttca aacatgtttg ataggtatcc cgtcccctat ttccgagggt gattttaagg
     5401 gatatgtttc tgatacaaat tgcaccacct tgggaactga tcggttagtc tcgtcagccg
     5461 actttactgg cggacctgac aacagtacca ccctcactta tcggaaggtc tcatgcttgt
     5521 tgttaaagct gaatgtctct atgtgggatg agccacctga actacagctg ttaggttccc
     5581 agtctctccc taacattact aatattgctc agatttccgg tataaccggg ggatgcgtag
     5641 gcttcagacc acaaggggtt ccttggtatc taggttggtc tagacaggag gccacgcggt
     5701 ttctccttag acacccctct ttctctaaat ccacggaacc gtttacggtg gtgacagcgg
     5761 ataggcacaa tctttttatg gggagtgagt actgcggtgc atatggctac agattttgga
     5821 acatgtataa ctgctcacag gtggggcggc agtaccgctg tggtaatgcg cgcagccccc
     5881 gcccgggtct tcctgaaatc cagtgtacaa ggagaggagg caaatgggtt aatcaatcac
     5941 aggaaattaa tgagtcggag ccgttcagct ttacggtgaa ctgtacagct agtagtttgg
     6001 gtaatgccag tgggtgttgc ggaaaagcag gcacgattct cccgggaaag tgggtcgaca
     6061 gcacacaagg tagtttcacc aaaccaaaag cgctaccacc cgcaattttc ctcatttgtg
     6121 gggatcgcgc atggcaagga attcccagtc gtccggtagg gggcccctgc tatttaggca
     6181 agcttaccat gttagcacct aagcatacag atattctcaa ggtgcttgtc aattcatcgc
     6241 ggacaggtat aagacgtaaa cgaagcacct cacacctgga tgatacatgc tcagatgaag
     6301 tgcagctttg gggtcctaca gcaagaatct ttgcatctat cctagccccg ggggtagcag
     6361 ctgcgcaagc cttaagagaa attgagagac tagcctgttg gtccgttaaa caggctaact
     6421 tgacaacatc actcctcggg gacttattgg atgatgtcac gagtattcga cacgcggtcc
     6481 tgcagaaccg agcggctatt gacttcttgc tcctagctca cggccatggc tgtgaggacg
     6541 ttgccggaat gtgctgtttc aatttgagtg atcacagtga gtctatacag aagaagttcc
     6601 agctaatgaa ggaacatgtc aataagatcg gcgtggacag cgacccaatt ggaagttggc
     6661 tgcgaggact attcggggga ataggagaat gggccgttca tttgctgaaa ggactgcttt
     6721 tggggcttgt agttattttg ttgctagtag tgtgcctgcc ttgccttttg caaatcgtat
     6781 gcggtaacat cagaaagatg attaataact ccatcagcta ccacacggaa tataagaagc
     6841 tgcaaaaggc ctgtgggcag cctgaaagca gaatagtata aggcagtaca tgggtggtgg
     6901 tatagcgctt gcgagtccat cgaggaaggc aggaaagaca gctattggta attgtgaaat
     6961 acgcttttgt ctgtgtgctg caggagctga gctgactctg ctggtggcct cgcgtaccac
     7021 tgtggcatcg ggccattacg gccgcggccg ccagtgtgat ggatatctgc agaattcggc
     7081 ttccaccatg gggagcagca agagcaagcc caaggacccc agccagcgcc caccccgacc
     7141 atcatctggt gaactatggg gcatccactt gatgcccccg aggatccttg tggagtgtct
     7201 tctcccaaat ggaatgatag tgactctaga atgcctccgt gaggccacac tgctaactat
     7261 caaacatgaa ctttttaaag aagcaagaaa ataccctctc tatcagctcc ttcaagatga
     7321 atcttcttac atttttgtaa gtgttacgca agaagcagaa agagaagaat tttttgatga
     7381 aacacggaga ctttgtgacc tgcggctatt tcaacctttc ctaaaagtca ttgaaccagt
     7441 aggtaacaga gaagaaaaga tccttaatag agaaataggt tttgctattg gcatgcccat
     7501 ctgtgagttt gacatggtta aggatcctga agtacaagat ttcagaagaa acattcttaa
     7561 tgtttgtaaa gaagcagtag atcttcgaga tgccaatgct ccacatagta gagcattata
     7621 tgtctgtcct ccaaatgtag aatcttcacc tgagctaccc aaacacatat acaataaact
     7681 agataaaggg caaataatag tggtgatatg ggtaatagtt tcacctaaca acgataagca
     7741 gaagtacacc ttaaaaatca atcatgactg tgtgcctgag caagttattg ctgaagcaat
     7801 taggaagaaa acacgaagta tgttgctgtc atctgaacaa ctgaagcttt gcgtgttgga
     7861 gtaccagggc aagtatattc tgaaagtgtg tggctgtgat gaatacttgc tagaaaaata
     7921 tccactgagc cagtataagt acataagaag ttgtataatg cttggtcgca tgcccaatct
     7981 catgctgatg gctaaagaaa gcctatatac ccagctgccg ctcgatacct ttacaatgcc
     8041 atcttactcc aggcgtatct ctacagctac accctacatg aatggagaag ctacagccaa
     8101 gtccctctgg actataaaca gtgctctcag aataagaatc ctctgtgcaa cctatgtaaa
     8161 tgtgaacatt agagacattg acaaaatcta cgttagaaca ggtatctacc atggaggaga
     8221 acctttatgt gacaatgtga atactcagag ggtaccttgt tctaatccca gatggaatga
     8281 atggctgtcg tatgacatgt acattcctga tcttccacgt gctgctcggc tctgcctctc
     8341 tatctgttct gttaaaggcc ggaagggtgc taaagaggag cactgtccat tggcttgggg
     8401 aaacataaac atgtttgact acacggacac tcttgtatct gggaaaatgg ctttgaatct
     8461 ttgggcagta ccccatggac tggaggattt gttgaatcct ataggtgtta ctggatcaaa
     8521 tccgaataag gaaactccat gtttagagct ggaatttgat tggtttagca atcctgttaa
     8581 gtttccagat atgacagtga ttgaagaaca tgccaattgg acgatctcac gtgaactggg
     8641 ctttaactac agctatgcgg gactgagtaa cagaatagct agagacaatg aattaagaga
     8701 aagtgacaag gagcaactga gagccatatg tacgcgagat cctttgtctg aaatcactga
     8761 gcaagagaag gacttcctct ggagccacag acactattgt gtaaatacgc cagaaattct
     8821 gcccaaatta cttctgtctg ttaaatggaa ttctagagat gaagtggctc agatgtactg
     8881 tttggtaaaa gattggcctc caatcaagcc agagcaagca atggagcttt tggattgtaa
     8941 ttatccagat ccaatggtgc gagcttttgc agttcggtgt ctagagaagt acttaacaga
     9001 tgacaaactg tctcagtact taatccagct agtacaggtt ctgaaatatg agcagtactt
     9061 agataatcaa ctcgtgagat ttttactcaa gaaggcactg accaatcaac ggataggaca
     9121 cttcttcttt tggcatttaa agtctgaaat gcacaataaa actgtaagtc agaggtttgg
     9181 tttacttctg gagtcctatt gtcgagcatg tggaatgtac ctaaagcatc tgagcaggca
     9241 ggtggaggct atggagaagt tgattaacct cacagatatt ctcaagcagg aaaagaaaga
     9301 tgagacccag aaggtgcaga tgaagtttct tgttgaacaa atgagacgtc cagattttat
     9361 ggatgcttta caaggcttta tctctcctct taatcctgct catcaactgg gaaatcttcg
     9421 gcttgaggag tgcagaataa tgtcatctgc aaaaaggccc ctgtggttaa actgggaaaa
     9481 cccagatatt atgtctgaat tgctatttca gaacaatgag ataatcttta aaaatggaga
     9541 tgacttgcgt caagacatgc tgacacttca gataattaga attatggaaa acatctggca
     9601 aaaccaaggt cttgatcttc ggatgttgcc ttacggttgt ttgtctattg gtgactgtgt
     9661 gggactcatt gaggtagtga gaagttctca tacaatcatg cagattcagt gtaaaggagg
     9721 cttaaaggga gcattgcagt tcaacagcca tacattgcat cagtggctca aggacaagaa
     9781 caaaggagaa atgtatgatg cagctattga cttgtttaca cgttcttgtg ctggctactg
     9841 tgtcgctacc tttatactgg gcattggtga tcgccacaac agtaacatca tggtgaaaga
     9901 tgatggacaa ctgtttcata ttgactttgg ccacttcctt gaccacaaga agaagaaatt
     9961 tggctacaaa agagagcgtg tgccctttgt cttaacacag gactttttaa tagtgattag
    10021 taaaggagcc caagaatgta ccaaaacaag ggagtttgaa aggtttcaag agatgtgtta
    10081 taaggcatat ctagcaattc ggcagcatgc caatctgttc ataaatctct tctccatgat
    10141 gcttggctca ggaatgccag aactgcagtc ctttgacgat attgcataca ttcgaaagac
    10201 ccttgcattg gacaaaactg agcaggaagc tcttgagtac ttcatgaagc aaatgaatga
    10261 tgctcaccat ggtggctgga caacaaaaat ggactggatc ttccacacaa taaagcaaca
    10321 tgctttgaac gactacaaag acgatgacga caagtgaaag ccgaattcca gcacactggc
    10381 ggccgttact agtggatccg atatcgaatt cacgcgtaag cttatcgatg tcgacgggcc
    10441 cggccgcctc ggcccgatgc gatgtacggg ccagatatac gcgtatctga ggggactagg
    10501 gtgtgtttag gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc ctcaggatat
    10561 agtagtttcg cttttgcata gggaggggga aatgtagtct tatgcaatac tcttgtagtc
    10621 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
    10681 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
    10741 tctgacatgg attggacgaa ccactgaatt ccgcattgca gagatattgt atttaagtgc
    10801 ctagctcgat acaataaacg ccatttgacc attcaccaca ttggtgtgca cctgggttga
    10861 tggccggacc gttgattccc tgacgactac gagcacctgc atgaagcaga aggcttcatt
    10921 tggtgacccc gacgtgatag ttagggaata gtggtcggcc acagacggcg tggcgatcct
    10981 gtctccatcc gtctcgtcta tcgggaggcg agttcgatga ccctggtgga gggggctgcg
    11041 gcttagggag gcagaagctg agtaccgtcg gaggggatcc acaggacggg tgtggtcgcc
    11101 atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca
    11161 aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc
    11221 gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg
    11281 cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg
    11341 atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac
    11401 tgtgataaac taccgcatta aagctccaag cttggctgtt tcctgtgtga aattgttatc
    11461 cgctcacaat tccacacatt atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    11521 aatgagtgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
    11581 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
    11641 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    11701 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    11761 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    11821 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    11881 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    11941 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
    12001 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    12061 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    12121 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    12181 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    12241 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
    12301 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    12361 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    12421 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    12481 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    12541 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    12601 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    12661 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    12721 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    12781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    12841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    12901 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    12961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    13021 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    13081 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    13141 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    13201 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    13261 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    13321 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    13381 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    13441 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    13501 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
    13561 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
    13621 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
    13681 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
    13741 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
    13801 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
    13861 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
    13921 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
    13981 gatgcctccg tgtaaggagg atttctgttc atgggggtaa tgataccgat gaaacgagag
    14041 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
    14101 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
    14161 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
    14221 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
    14281 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
    14341 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
    14401 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg
    14461 ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc
    14521 caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga
    14581 gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc
    14641 atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgatg cgatgtacgg
    14701 gccagatata cgcgtatctg aggggactag ggtgtgttta ggcgaaaagc ggggcttcgg
    14761 ttgtacgcgg ttaggagtcc cctcaggata tagtagtttc gcttttgcat agggaggggg
    14821 aaatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
    14881 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
    14941 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
    15001 tccgcattgc agagatattg tatttaagtg cctagctcga tacaataaac
//
